Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 3104-3107 |
| Numero di pagine | 4 |
| Rivista | Analytical Chemistry |
| Volume | 82 |
| Stato di pubblicazione | Published - 2010 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Inkjet printing methodologies for drug screening. / Pignataro, Bruno Giuseppe; Arrabito, Giuseppe Domenico; Arrabito, Giuseppe.
In: Analytical Chemistry, Vol. 82, 2010, pag. 3104-3107.Risultato della ricerca: Article
}
TY - JOUR
T1 - Inkjet printing methodologies for drug screening
AU - Pignataro, Bruno Giuseppe
AU - Arrabito, Giuseppe Domenico
AU - Arrabito, Giuseppe
PY - 2010
Y1 - 2010
N2 - We show for the first time a contactless, low-cost, and rapid drug screening methodology by employing inkjet printing for molecular dispensing in a microarray format. Picoliter drops containing a model substrate (D-glucose)/ inhibitor (D-glucal) couple were accurately dispensed on a single layer consisting of the enzymatic target (glucose oxidase) covalently linked to a functionalized silicon oxide support. A simple colorimetric detection method allowed one to prove the screening capability of the microarray with the possibility to assay with high reproducibility at the single spot level. Measurements of the optical signal as a function of concentration and of time verified the occurrence at the solid-liquid interface of the competitive enzymatic inhibition with a similar behavior occurring for this system in a solution phase along with overcoming competition effects. We propose this methodology as a general application for drug screening purposes, since it may be extended to any kind of enzyme-substrate/ inhibitor or ligand-target biochemical system
AB - We show for the first time a contactless, low-cost, and rapid drug screening methodology by employing inkjet printing for molecular dispensing in a microarray format. Picoliter drops containing a model substrate (D-glucose)/ inhibitor (D-glucal) couple were accurately dispensed on a single layer consisting of the enzymatic target (glucose oxidase) covalently linked to a functionalized silicon oxide support. A simple colorimetric detection method allowed one to prove the screening capability of the microarray with the possibility to assay with high reproducibility at the single spot level. Measurements of the optical signal as a function of concentration and of time verified the occurrence at the solid-liquid interface of the competitive enzymatic inhibition with a similar behavior occurring for this system in a solution phase along with overcoming competition effects. We propose this methodology as a general application for drug screening purposes, since it may be extended to any kind of enzyme-substrate/ inhibitor or ligand-target biochemical system
KW - drug screening, inkjet printing, microarrays, biological surfaces
UR - http://hdl.handle.net/10447/49712
M3 - Article
VL - 82
SP - 3104
EP - 3107
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
ER -