Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 3104-3107 |
| Numero di pagine | 4 |
| Rivista | Analytical Chemistry |
| Volume | 82 |
| Stato di pubblicazione | Published - 2010 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Inkjet printing methodologies for drug screening. / Pignataro, Bruno Giuseppe; Arrabito, Giuseppe Domenico; Arrabito, Giuseppe.
In: Analytical Chemistry, Vol. 82, 2010, pag. 3104-3107.Risultato della ricerca: Article
}
TY - JOUR
T1 - Inkjet printing methodologies for drug screening
AU - Pignataro, Bruno Giuseppe
AU - Arrabito, Giuseppe Domenico
AU - Arrabito, Giuseppe
PY - 2010
Y1 - 2010
N2 - We show for the first time a contactless, low-cost, andrapid drug screening methodology by employing inkjetprinting for molecular dispensing in a microarray format.Picoliter drops containing a model substrate (D-glucose)/inhibitor (D-glucal) couple were accurately dispensed ona single layer consisting of the enzymatic target (glucoseoxidase) covalently linked to a functionalized silicon oxidesupport. A simple colorimetric detection method allowedone to prove the screening capability of the microarraywith the possibility to assay with high reproducibility atthe single spot level. Measurements of the optical signalas a function of concentration and of time verified theoccurrence at the solid-liquid interface of the competitiveenzymatic inhibition with a similar behavior occurring forthis system in a solution phase along with overcomingcompetition effects. We propose this methodology as ageneral application for drug screening purposes, since itmay be extended to any kind of enzyme-substrate/inhibitor or ligand-target biochemical system
AB - We show for the first time a contactless, low-cost, andrapid drug screening methodology by employing inkjetprinting for molecular dispensing in a microarray format.Picoliter drops containing a model substrate (D-glucose)/inhibitor (D-glucal) couple were accurately dispensed ona single layer consisting of the enzymatic target (glucoseoxidase) covalently linked to a functionalized silicon oxidesupport. A simple colorimetric detection method allowedone to prove the screening capability of the microarraywith the possibility to assay with high reproducibility atthe single spot level. Measurements of the optical signalas a function of concentration and of time verified theoccurrence at the solid-liquid interface of the competitiveenzymatic inhibition with a similar behavior occurring forthis system in a solution phase along with overcomingcompetition effects. We propose this methodology as ageneral application for drug screening purposes, since itmay be extended to any kind of enzyme-substrate/inhibitor or ligand-target biochemical system
KW - biological surfaces
KW - drug screening
KW - inkjet printing
KW - microarrays
UR - http://hdl.handle.net/10447/49712
M3 - Article
VL - 82
SP - 3104
EP - 3107
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
ER -