Inkjet printing methodologies for drug screening

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44 Citazioni (Scopus)

Abstract

We show for the first time a contactless, low-cost, andrapid drug screening methodology by employing inkjetprinting for molecular dispensing in a microarray format.Picoliter drops containing a model substrate (D-glucose)/inhibitor (D-glucal) couple were accurately dispensed ona single layer consisting of the enzymatic target (glucoseoxidase) covalently linked to a functionalized silicon oxidesupport. A simple colorimetric detection method allowedone to prove the screening capability of the microarraywith the possibility to assay with high reproducibility atthe single spot level. Measurements of the optical signalas a function of concentration and of time verified theoccurrence at the solid-liquid interface of the competitiveenzymatic inhibition with a similar behavior occurring forthis system in a solution phase along with overcomingcompetition effects. We propose this methodology as ageneral application for drug screening purposes, since itmay be extended to any kind of enzyme-substrate/inhibitor or ligand-target biochemical system
Lingua originaleEnglish
pagine (da-a)3104-3107
Numero di pagine4
RivistaAnalytical Chemistry
Volume82
Stato di pubblicazionePublished - 2010

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Printing
Screening
Pharmaceutical Preparations
Calcium Gluconate
Silicon
Substrates
Microarrays
Assays
Ligands
Glucose
Liquids
Enzymes
Costs

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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Inkjet printing methodologies for drug screening. / Pignataro, Bruno Giuseppe; Arrabito, Giuseppe Domenico; Arrabito, Giuseppe.

In: Analytical Chemistry, Vol. 82, 2010, pag. 3104-3107.

Risultato della ricerca: Article

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AB - We show for the first time a contactless, low-cost, andrapid drug screening methodology by employing inkjetprinting for molecular dispensing in a microarray format.Picoliter drops containing a model substrate (D-glucose)/inhibitor (D-glucal) couple were accurately dispensed ona single layer consisting of the enzymatic target (glucoseoxidase) covalently linked to a functionalized silicon oxidesupport. A simple colorimetric detection method allowedone to prove the screening capability of the microarraywith the possibility to assay with high reproducibility atthe single spot level. Measurements of the optical signalas a function of concentration and of time verified theoccurrence at the solid-liquid interface of the competitiveenzymatic inhibition with a similar behavior occurring forthis system in a solution phase along with overcomingcompetition effects. We propose this methodology as ageneral application for drug screening purposes, since itmay be extended to any kind of enzyme-substrate/inhibitor or ligand-target biochemical system

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