11 Citazioni (Scopus)

Abstract

N-valproyl-L-tryptophan (VPA-Tryp), new antiepileptic drug, was tested on CA1 hippocampal epileptiform bursting activity obtained by increasing potassium and lowering calcium and magnesium concentrations in the fluid perfusing rat brain slices. Slices were treated with 0.5, 1 or 2 mM Valproate (VPA) or 0.2, 0.5 or 1 mM VPA-Tryp. Both burst duration and interburst frequency during and after treatment were off-line compared with baseline values. For both parameters, the latency and the length of statistically significant response periods, as well as the magnitude of drug-induced responses were calculated. VPA-Tryp evoked fewer and weaker early excitatory effects than VPA on bursting activity. On the contrary, VPA-Tryp induced powerful and long-lasting inhibitory effects on epileptiform discharge in a significantly higher number of slices than VPA. In fact, greater length and magnitude of VPA-Tryp-induced inhibition on both interburst frequency and burst duration were observed. Furthermore, VPA-Tryp showed antiepileptic activity at lower concentration than VPA and, when testing both drugs at analogue concentrations, VPA-Tryp evoked responses with either shorter latency or greater effect length and magnitude than VPA.
Lingua originaleEnglish
pagine (da-a)1249-1259
Numero di pagine11
RivistaJournal of Neural Transmission
Volume119
Stato di pubblicazionePublished - 2012

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Valproic Acid
Magnesium
Potassium
Calcium
Brain
Anticonvulsants
N-valproyl-L-tryptophan
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biological Psychiatry
  • Psychiatry and Mental health
  • Clinical Neurology
  • Neurology

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@article{287eb72d620e4d388e3df55e907cfe0f,
title = "Inhibitory effects of N-valproyl-L-tryptophan on high potassium, low calcium and low magnesium-induced CA1 hippocampal epileptiform bursting activity in rat brain slices",
abstract = "N-valproyl-L-tryptophan (VPA-Tryp), new antiepileptic drug, was tested on CA1 hippocampal epileptiform bursting activity obtained by increasing potassium and lowering calcium and magnesium concentrations in the fluid perfusing rat brain slices. Slices were treated with 0.5, 1 or 2 mM Valproate (VPA) or 0.2, 0.5 or 1 mM VPA-Tryp. Both burst duration and interburst frequency during and after treatment were off-line compared with baseline values. For both parameters, the latency and the length of statistically significant response periods, as well as the magnitude of drug-induced responses were calculated. VPA-Tryp evoked fewer and weaker early excitatory effects than VPA on bursting activity. On the contrary, VPA-Tryp induced powerful and long-lasting inhibitory effects on epileptiform discharge in a significantly higher number of slices than VPA. In fact, greater length and magnitude of VPA-Tryp-induced inhibition on both interburst frequency and burst duration were observed. Furthermore, VPA-Tryp showed antiepileptic activity at lower concentration than VPA and, when testing both drugs at analogue concentrations, VPA-Tryp evoked responses with either shorter latency or greater effect length and magnitude than VPA.",
author = "Giannola, {Libero Italo} and Pierangelo Sardo and Giuseppe Ferraro and {De Caro}, Viviana and Giulia Giandalia and Fabio Carletti and Valerio Rizzo and Simonetta Friscia and Scaturro, {Anna Lisa}",
year = "2012",
language = "English",
volume = "119",
pages = "1249--1259",
journal = "Journal of Neural Transmission",
issn = "0300-9564",
publisher = "Springer Verlag",

}

TY - JOUR

T1 - Inhibitory effects of N-valproyl-L-tryptophan on high potassium, low calcium and low magnesium-induced CA1 hippocampal epileptiform bursting activity in rat brain slices

AU - Giannola, Libero Italo

AU - Sardo, Pierangelo

AU - Ferraro, Giuseppe

AU - De Caro, Viviana

AU - Giandalia, Giulia

AU - Carletti, Fabio

AU - Rizzo, Valerio

AU - Friscia, Simonetta

AU - Scaturro, Anna Lisa

PY - 2012

Y1 - 2012

N2 - N-valproyl-L-tryptophan (VPA-Tryp), new antiepileptic drug, was tested on CA1 hippocampal epileptiform bursting activity obtained by increasing potassium and lowering calcium and magnesium concentrations in the fluid perfusing rat brain slices. Slices were treated with 0.5, 1 or 2 mM Valproate (VPA) or 0.2, 0.5 or 1 mM VPA-Tryp. Both burst duration and interburst frequency during and after treatment were off-line compared with baseline values. For both parameters, the latency and the length of statistically significant response periods, as well as the magnitude of drug-induced responses were calculated. VPA-Tryp evoked fewer and weaker early excitatory effects than VPA on bursting activity. On the contrary, VPA-Tryp induced powerful and long-lasting inhibitory effects on epileptiform discharge in a significantly higher number of slices than VPA. In fact, greater length and magnitude of VPA-Tryp-induced inhibition on both interburst frequency and burst duration were observed. Furthermore, VPA-Tryp showed antiepileptic activity at lower concentration than VPA and, when testing both drugs at analogue concentrations, VPA-Tryp evoked responses with either shorter latency or greater effect length and magnitude than VPA.

AB - N-valproyl-L-tryptophan (VPA-Tryp), new antiepileptic drug, was tested on CA1 hippocampal epileptiform bursting activity obtained by increasing potassium and lowering calcium and magnesium concentrations in the fluid perfusing rat brain slices. Slices were treated with 0.5, 1 or 2 mM Valproate (VPA) or 0.2, 0.5 or 1 mM VPA-Tryp. Both burst duration and interburst frequency during and after treatment were off-line compared with baseline values. For both parameters, the latency and the length of statistically significant response periods, as well as the magnitude of drug-induced responses were calculated. VPA-Tryp evoked fewer and weaker early excitatory effects than VPA on bursting activity. On the contrary, VPA-Tryp induced powerful and long-lasting inhibitory effects on epileptiform discharge in a significantly higher number of slices than VPA. In fact, greater length and magnitude of VPA-Tryp-induced inhibition on both interburst frequency and burst duration were observed. Furthermore, VPA-Tryp showed antiepileptic activity at lower concentration than VPA and, when testing both drugs at analogue concentrations, VPA-Tryp evoked responses with either shorter latency or greater effect length and magnitude than VPA.

UR - http://hdl.handle.net/10447/74288

M3 - Article

VL - 119

SP - 1249

EP - 1259

JO - Journal of Neural Transmission

JF - Journal of Neural Transmission

SN - 0300-9564

ER -