Inhibition of the mechanical activity of mouse ileum by cactus pear (Opuntia Ficus Indica, L, Mill.) fruit extract and its pigment indicaxanthin.

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Abstract

We investigated, using an organ bath technique, the effects of a hydrophilic extract from Opuntiaficus indica fruit pulp (cactus fruit extract, CFE) on the motility of mouse ileum, and researched theextract component(s) responsible for the observed responses. CFE (10-320 mg of fresh fruit pulpequivalents/mL of organ bath) reduced dose-dependently the spontaneous contractions. This effectwas unaffected by tetrodotoxin, a neuronal blocker, Nω-nitro-L-arginine methyl ester, a nitric oxidesynthase blocker, tetraethylammonium, a potassium channel blocker, or atropine, a muscarinicreceptor antagonist. CFE also reduced the contractions evoked by carbachol, without affecting thecontractions evoked by high extracellular potassium. Indicaxanthin, but not ascorbic acid, assayedat concentrations comparable with their content in CFE, mimicked the CFE effects. The data showthat CFE is able to exert direct antispasmodic effects on the intestinal motility. The CFE inhibitoryeffects do not involve potassium channels or voltage-dependent calcium channels but ratherpathways of calcium intracellular release. The fruit pigment indicaxanthin appears to be the maincomponent responsible for the CFE-induced effects.
Lingua originaleEnglish
pagine (da-a)7565-7571
Numero di pagine7
RivistaJournal of Agricultural and Food Chemistry
Volume58
Stato di pubblicazionePublished - 2010

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Opuntia
Cactaceae
cactus pears
Pyrus
Opuntia ficus-indica
fruit extracts
Fruits
Ileum
ileum
Pigments
Fruit
pigments
mice
potassium channels
Baths
parasympatholytics
carbachol
tetrodotoxin
gastrointestinal motility
calcium channels

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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@article{ea389b70c0894eb58e9e40c78c45ef3a,
title = "Inhibition of the mechanical activity of mouse ileum by cactus pear (Opuntia Ficus Indica, L, Mill.) fruit extract and its pigment indicaxanthin.",
abstract = "We investigated, using an organ bath technique, the effects of a hydrophilic extract from Opuntiaficus indica fruit pulp (cactus fruit extract, CFE) on the motility of mouse ileum, and researched theextract component(s) responsible for the observed responses. CFE (10-320 mg of fresh fruit pulpequivalents/mL of organ bath) reduced dose-dependently the spontaneous contractions. This effectwas unaffected by tetrodotoxin, a neuronal blocker, Nω-nitro-L-arginine methyl ester, a nitric oxidesynthase blocker, tetraethylammonium, a potassium channel blocker, or atropine, a muscarinicreceptor antagonist. CFE also reduced the contractions evoked by carbachol, without affecting thecontractions evoked by high extracellular potassium. Indicaxanthin, but not ascorbic acid, assayedat concentrations comparable with their content in CFE, mimicked the CFE effects. The data showthat CFE is able to exert direct antispasmodic effects on the intestinal motility. The CFE inhibitoryeffects do not involve potassium channels or voltage-dependent calcium channels but ratherpathways of calcium intracellular release. The fruit pigment indicaxanthin appears to be the maincomponent responsible for the CFE-induced effects.",
keywords = "antispasmodic effect, indicaxanthin, intestinal smooth muscle, opuntia ficus indica",
author = "Livrea, {Maria Antonia} and Flavia Mule' and Sara Baldassano and Luisa Tesoriere and Serio, {Rosa Maria} and Alessandra Rotondo",
year = "2010",
language = "English",
volume = "58",
pages = "7565--7571",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Inhibition of the mechanical activity of mouse ileum by cactus pear (Opuntia Ficus Indica, L, Mill.) fruit extract and its pigment indicaxanthin.

AU - Livrea, Maria Antonia

AU - Mule', Flavia

AU - Baldassano, Sara

AU - Tesoriere, Luisa

AU - Serio, Rosa Maria

AU - Rotondo, Alessandra

PY - 2010

Y1 - 2010

N2 - We investigated, using an organ bath technique, the effects of a hydrophilic extract from Opuntiaficus indica fruit pulp (cactus fruit extract, CFE) on the motility of mouse ileum, and researched theextract component(s) responsible for the observed responses. CFE (10-320 mg of fresh fruit pulpequivalents/mL of organ bath) reduced dose-dependently the spontaneous contractions. This effectwas unaffected by tetrodotoxin, a neuronal blocker, Nω-nitro-L-arginine methyl ester, a nitric oxidesynthase blocker, tetraethylammonium, a potassium channel blocker, or atropine, a muscarinicreceptor antagonist. CFE also reduced the contractions evoked by carbachol, without affecting thecontractions evoked by high extracellular potassium. Indicaxanthin, but not ascorbic acid, assayedat concentrations comparable with their content in CFE, mimicked the CFE effects. The data showthat CFE is able to exert direct antispasmodic effects on the intestinal motility. The CFE inhibitoryeffects do not involve potassium channels or voltage-dependent calcium channels but ratherpathways of calcium intracellular release. The fruit pigment indicaxanthin appears to be the maincomponent responsible for the CFE-induced effects.

AB - We investigated, using an organ bath technique, the effects of a hydrophilic extract from Opuntiaficus indica fruit pulp (cactus fruit extract, CFE) on the motility of mouse ileum, and researched theextract component(s) responsible for the observed responses. CFE (10-320 mg of fresh fruit pulpequivalents/mL of organ bath) reduced dose-dependently the spontaneous contractions. This effectwas unaffected by tetrodotoxin, a neuronal blocker, Nω-nitro-L-arginine methyl ester, a nitric oxidesynthase blocker, tetraethylammonium, a potassium channel blocker, or atropine, a muscarinicreceptor antagonist. CFE also reduced the contractions evoked by carbachol, without affecting thecontractions evoked by high extracellular potassium. Indicaxanthin, but not ascorbic acid, assayedat concentrations comparable with their content in CFE, mimicked the CFE effects. The data showthat CFE is able to exert direct antispasmodic effects on the intestinal motility. The CFE inhibitoryeffects do not involve potassium channels or voltage-dependent calcium channels but ratherpathways of calcium intracellular release. The fruit pigment indicaxanthin appears to be the maincomponent responsible for the CFE-induced effects.

KW - antispasmodic effect

KW - indicaxanthin

KW - intestinal smooth muscle

KW - opuntia ficus indica

UR - http://hdl.handle.net/10447/53356

M3 - Article

VL - 58

SP - 7565

EP - 7571

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

ER -