Abstract
Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Lingua originale | English |
---|---|
pagine (da-a) | 19-25 |
Numero di pagine | 7 |
Rivista | BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH. |
Volume | 47 |
Stato di pubblicazione | Published - 1989 |
All Science Journal Classification (ASJC) codes
- Developmental Neuroscience
- Developmental Biology