Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

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Abstract

Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Lingua originaleEnglish
pagine (da-a)19-25
Numero di pagine7
RivistaBRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH.
Volume47
Stato di pubblicazionePublished - 1989

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All Science Journal Classification (ASJC) codes

  • Developmental Neuroscience
  • Developmental Biology

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