Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

Michela Giuliano; Gennaro Taibi; Giuseppe Calvaruso; Renza Vento

Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

Michela Giuliano, Gennaro Taibi, Giuseppe Calvaruso, Renza Vento

Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche

Risultato della ricerca: Article › peer review

4 Citazioni (Scopus)

Abstract

Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.

Lingua originale	English
pagine (da-a)	19-25
Numero di pagine	7
Rivista	BRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH.
Volume	47
Stato di pubblicazione	Published - 1989

All Science Journal Classification (ASJC) codes

Developmental Neuroscience
Developmental Biology

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title = "Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum",

abstract = "Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.",

author = "Michela Giuliano and Gennaro Taibi and Giuseppe Calvaruso and Renza Vento",

year = "1989",

language = "English",

volume = "47",

pages = "19--25",

journal = "Developmental Brain Research",

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T1 - Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

AU - Giuliano, Michela

AU - Taibi, Gennaro

AU - Calvaruso, Giuseppe

AU - Vento, Renza

PY - 1989

Y1 - 1989

N2 - Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.

AB - Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.

UR - http://hdl.handle.net/10447/65746

M3 - Article

VL - 47

SP - 19

EP - 25

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

ER -