Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

Tesoriere, G.; Schiavo, M.

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.
Lingua originaleEnglish
pagine (da-a)19-25
Numero di pagine7
RivistaBRAIN RESEARCH. DEVELOPMENTAL BRAIN RESEARCH.
Volume47
Stato di pubblicazionePublished - 1989

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Developmental Neuroscience

Cita questo

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title = "Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum",
abstract = "Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45{\%} saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50{\%} inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.",
author = "{Tesoriere, G.; Schiavo, M.} and Giuseppe Calvaruso and Renza Vento and Gennaro Taibi and Michela Giuliano",
year = "1989",
language = "English",
volume = "47",
pages = "19--25",
journal = "Developmental Brain Research",
issn = "0165-3806",
publisher = "Elsevier BV",

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TY - JOUR

T1 - Inhibition of DNA synthesis in chick embryo retinas, in vitro, by a factor from fetal bovine serum

AU - Tesoriere, G.; Schiavo, M.

AU - Calvaruso, Giuseppe

AU - Vento, Renza

AU - Taibi, Gennaro

AU - Giuliano, Michela

PY - 1989

Y1 - 1989

N2 - Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.

AB - Fetal bovine serum inhibited deoxyribonucleic acid (DNA) synthesis in chick embryo retina explants. The inhibitory activity was precipitated from fetal bovine serum by 45% saturated ammonium sulfate and isolated by means of Sephadex G-100 and Bio-Gel P-60 columns as a peak with an apparent molecular weight of 7000 Da. DNA-inhibiting activity was heat- and acid-stable and was destroyed by dithiothreitol and alkaline treatment. The purified factor inhibited similarly both DNA synthesis and thymidine kinase activity; 50% inhibitory effect was found with 160 ng, 17 h after the addition into the incubation medium.

UR - http://hdl.handle.net/10447/65746

M3 - Article

VL - 47

SP - 19

EP - 25

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

ER -