Influence of roasting and different brewing processes on ochratoxin A content in coffee determined by high-performance liquid chromatography-fluorescence detection (HPLC-FLD)

Giuseppe Avellone, Pasquale Agozzino, Giuseppa Di Di Bella, Pasquale Agozzino, Lara La Pera, Giuseppe Avellone, Giacomo Dugo, Vincenzo Lo Lo Turco

Risultato della ricerca: Article

25 Citazioni (Scopus)

Abstract

A rapid and reliable procedure has been developed for the determination of ochratoxin A (OTA) in green androasted coffee. The method consists of extraction of the sample with methanol–5% aqueous sodium hydrogencarbonate/1% PEG8000 (20:80), followed by immunoaffinity column (IAC) clean-up and, finally, highperformanceliquid chromatography (HPLC) determination with fluorimetric detection. Mean recoveries forgreen and roasted coffee spiked at different levels ranging from 94 and 105% were obtained. The limit ofdetermination (S/N¼3) was 0.032 ng g 1 and the precision (within-laboratory relative standard deviation) was6%. The method described has been used to assess the influence of roasting and different brewing processes onOTA content in commercial lots of green and roasted coffee. The results provided evidence that roasting led to asignificant drop on OTA levels (65–100%). Also, the way coffee is prepared affects the OTA content: brewingusing a Moka Express (Italian coffee) led to a significant reduction of OTA concentration (50–75%) since hotwater stays in contact with coffee for a short time. On the contrary, Turkish coffee-making (infusion for about10 min) cause poor reduction in OTA.
Lingua originaleEnglish
pagine (da-a)1257-1263
Numero di pagine7
RivistaFOOD ADDITIVES & CONTAMINANTS. PART A. CHEMISTRY, ANALYSIS, CONTROL, EXPOSURE & RISK ASSESSMENT
Volume25
Stato di pubblicazionePublished - 2008

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All Science Journal Classification (ASJC) codes

  • Food Science
  • Chemistry(all)
  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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