Influence of nerve growth factor in endometriosis-associated symptoms

Vito Chiantera, Sylvia Mechsner, Vito Chiantera, Andreas D. Ebert, Maria L. Barcena De Arellano, Giuseppe F. Vercellino, Julia Arnold, Achim Schneider

Risultato della ricerca: Article

28 Citazioni (Scopus)

Abstract

To investigate the role of the nerve growth factor (NGF) in the development of dysmenorrhea/pelvic pain in patients with endometriosis, we performed a prospective, clinical, blind study. Peritoneal fluids (PFs) were obtained from patients with histologically proven endometriosis. Patients with endometriosis were divided into 7 different groups depending on their preoperative pain score and symptomatology: patients with no pain, patients with minimal pain (dysmenorrhea, pelvic pain, or both), and patients with severe pain (dysmenorrhea, pelvic pain, or both) and were used for the neuronal growth assay with cultured chicken dorsal root ganglia (DRG) and for Western blot analyses. Dorsal root ganglia were stained with anti-calcitonin gene-related peptide (CGRP) and anti-growth-associated protein 43 (GAP 43). Peritoneal fluids from patients with endometriosis induce neurite outgrowth. There was no significant difference in the outgrowth between the 7 pain groups. Western blot analyses showed a moderate NGF expression in the PFs from patients with endometriosis, without significant differences in the 7 pain groups. The present study suggests that the neurotrophic properties of endometriotic tissues are endometriosis- and not pain-associated. © Society for Gynecologic Investigation 2011.
Lingua originaleEnglish
pagine (da-a)1202-1210
Numero di pagine9
RivistaReproductive Sciences
Volume18
Stato di pubblicazionePublished - 2011

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Nerve Growth Factor
Endometriosis
Pain
Dysmenorrhea
Pelvic Pain
Ascitic Fluid
Spinal Ganglia
Western Blotting
GAP-43 Protein
Calcitonin Gene-Related Peptide
Chickens
Growth

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cita questo

Chiantera, V., Mechsner, S., Chiantera, V., Ebert, A. D., Barcena De Arellano, M. L., Vercellino, G. F., ... Schneider, A. (2011). Influence of nerve growth factor in endometriosis-associated symptoms. Reproductive Sciences, 18, 1202-1210.

Influence of nerve growth factor in endometriosis-associated symptoms. / Chiantera, Vito; Mechsner, Sylvia; Chiantera, Vito; Ebert, Andreas D.; Barcena De Arellano, Maria L.; Vercellino, Giuseppe F.; Arnold, Julia; Schneider, Achim.

In: Reproductive Sciences, Vol. 18, 2011, pag. 1202-1210.

Risultato della ricerca: Article

Chiantera, V, Mechsner, S, Chiantera, V, Ebert, AD, Barcena De Arellano, ML, Vercellino, GF, Arnold, J & Schneider, A 2011, 'Influence of nerve growth factor in endometriosis-associated symptoms', Reproductive Sciences, vol. 18, pagg. 1202-1210.
Chiantera V, Mechsner S, Chiantera V, Ebert AD, Barcena De Arellano ML, Vercellino GF e altri. Influence of nerve growth factor in endometriosis-associated symptoms. Reproductive Sciences. 2011;18:1202-1210.
Chiantera, Vito ; Mechsner, Sylvia ; Chiantera, Vito ; Ebert, Andreas D. ; Barcena De Arellano, Maria L. ; Vercellino, Giuseppe F. ; Arnold, Julia ; Schneider, Achim. / Influence of nerve growth factor in endometriosis-associated symptoms. In: Reproductive Sciences. 2011 ; Vol. 18. pagg. 1202-1210.
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AU - Mechsner, Sylvia

AU - Chiantera, Vito

AU - Ebert, Andreas D.

AU - Barcena De Arellano, Maria L.

AU - Vercellino, Giuseppe F.

AU - Arnold, Julia

AU - Schneider, Achim

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AB - To investigate the role of the nerve growth factor (NGF) in the development of dysmenorrhea/pelvic pain in patients with endometriosis, we performed a prospective, clinical, blind study. Peritoneal fluids (PFs) were obtained from patients with histologically proven endometriosis. Patients with endometriosis were divided into 7 different groups depending on their preoperative pain score and symptomatology: patients with no pain, patients with minimal pain (dysmenorrhea, pelvic pain, or both), and patients with severe pain (dysmenorrhea, pelvic pain, or both) and were used for the neuronal growth assay with cultured chicken dorsal root ganglia (DRG) and for Western blot analyses. Dorsal root ganglia were stained with anti-calcitonin gene-related peptide (CGRP) and anti-growth-associated protein 43 (GAP 43). Peritoneal fluids from patients with endometriosis induce neurite outgrowth. There was no significant difference in the outgrowth between the 7 pain groups. Western blot analyses showed a moderate NGF expression in the PFs from patients with endometriosis, without significant differences in the 7 pain groups. The present study suggests that the neurotrophic properties of endometriotic tissues are endometriosis- and not pain-associated. © Society for Gynecologic Investigation 2011.

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