Inflammatory Response to a High Radiation Dose in Breast Cancer

    Risultato della ricerca: Otherpeer review


    Background: Radiation therapy (RT) is an essential treatment modalityused for breast cancer (BC) care. Radiations can stimulate the immunesystem via the early activation of cytokine cascades, which can greatlyaffect cellular radio-sensitivity. Our aim is to analyze inflammatoryresponse induced by high ionizing radiation (IR) doses, generated byintraoperative radiotherapy (IORT) treatment, to identify several potentialtargets that may influence cell radio-response.Methods: MCF10, MCF7 and MDA-MB231 BC cell lines have beenexposed to high IR (23 Gray and 9 Gray), delivered by IORT treatments.Conditioned media (CM) were assayed at the time points: 0’, 30’, 1, 3, 6,24, 48, and 72 hours, for production of cytokines, chemokines and growthfactors by using Luminex technology.Results: We observed a very low cytokine concentration in MCF7 CMand a greater expression, of TH1 and TH2 cytokines, IL-7, G-CSF andMCP-1 in MCF10A CM. MDA-MB231 CM showed a reduced expressionof TH1 and TH2 cytokines, IL-7, G-CSF, MCP-1 and an up-regulation ofGM-CSF, IL-6 and IL-8 factors.Conclusions: Our results indicate that cytokine production in most casesis dose independent and time and cell type dependent. In particular, anover production at 24 hours after IORT treatment was observed. Inaddition, we speculate that MCF10A cells potentially exhibit an increasedcapacity to activate the immune system after IR exposure, and that MDAMB231cells could have a role in radio-resistance phenotype through IL-6and IL-8 high production activated by IR.
    Lingua originaleEnglish
    Numero di pagine1
    Stato di pubblicazionePublished - 2014


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