In situ hybridisation and mmunohistochemistryanalyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFα), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of development.Granulocytes with large granules and compartment/morula cells are CiTNFα-producing cells in both inflamed pharynxand larvae. Pharynx vessel endothelium also takes part in the inflammatory response. Haemocyte nodules in the vessel lumen or associated with the endothelium suggest theinvolvement of CiTNFα in recruiting lymphocyte-like cells and promoting the differentiation of inflammatory haemocytes.Specific antibodies against a CiTNFα peptide have identified a 43-kDa cell-bound form of the protein. Observations of pharynx histological sections (at 4 and 8 h post-LPSinoculation) from naive and medium-inoculated ascidians have confirmed the CiTNFα-positive tissue response. Larvalhistological sections and whole-mount preparations have revealed that CiTNFα is expressed by trunk mesenchyme,preoral lobe and tunic cells, indicating CiTNFα-expressing cell immigration events and an ontogenetic role.
|Numero di pagine||13|
|Rivista||Cell and Tissue Research|
|Stato di pubblicazione||Published - 2010|
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