Inducible lectins with galectin properties and human IL1 alpha epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis

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Abstract

Hemocytes from the ascidian Ciona intestinalis exert in vitro Ca2+-dependent cytotoxic activitytoward mammalian erythrocytes and K562 cells. To examine the lytic mechanism, hemocytepopulations were separated (B1-B6 bands) through a Percoll discontinuous density gradient, thehemocyte cytotoxic activity (HCA) and the lytic activity of the hemocyte lysate supernatant (HLS)were assayed. In addition the separated hemocytes were cultured and the cell free medium (CFM)assayed after 3h culture. Results support that unilocular refractile hemocytes (URGs), enriched inB5, are cytotoxic. The B5-HLS contains lysins and the activity of B5-CFM shows that lysisns canbe released into a culture medium. The B5 activity was blocked by D-Galactose, α-Lactose,Lactulose, LacNAc, thiodigalactoside (TDG), L-Fucose, D-Mannose, D-Glucose, sphingomyelin(SM), and soluble phospholipase A2 (sPLA2) inhibitors (dibucain, quinacrine). Accordingly, HLSchemico-physical properties (alkaline medium, high termostability, Ca2+-dependence, trypsintreatment, protease inhibitors) and SEM observations of the affected targets suggested that sPLA2could be responsible for changes and large alterations of the target cell membrane. An apoptoticactivity, as recorded by a caspase 3, 7 assay, was found by treating K562 cells with very dilutedHLS. A lytic mechanism involving sPLA2 and lectins promptly released by URGs and morula cellsrespectively is suggested, whereas target cell membrane SM could be a modulator of the enzymeactivity.
Lingua originaleEnglish
pagine (da-a)379-390
Numero di pagine12
RivistaCell and Tissue Research
Volume329
Stato di pubblicazionePublished - 2007

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Ciona intestinalis
Galectins
Hemocytes
Urochordata
Lectins
Epitopes
Yeasts
K562 Cells
Sphingomyelins
Phospholipase A2 Inhibitors
Cell Membrane
Caspase 7
Lactulose
Morula
Quinacrine
Fucose
Phospholipases A2
Lactose
Mannose
Protease Inhibitors

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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title = "Inducible lectins with galectin properties and human IL1 alpha epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis",
abstract = "Hemocytes from the ascidian Ciona intestinalis exert in vitro Ca2+-dependent cytotoxic activitytoward mammalian erythrocytes and K562 cells. To examine the lytic mechanism, hemocytepopulations were separated (B1-B6 bands) through a Percoll discontinuous density gradient, thehemocyte cytotoxic activity (HCA) and the lytic activity of the hemocyte lysate supernatant (HLS)were assayed. In addition the separated hemocytes were cultured and the cell free medium (CFM)assayed after 3h culture. Results support that unilocular refractile hemocytes (URGs), enriched inB5, are cytotoxic. The B5-HLS contains lysins and the activity of B5-CFM shows that lysisns canbe released into a culture medium. The B5 activity was blocked by D-Galactose, α-Lactose,Lactulose, LacNAc, thiodigalactoside (TDG), L-Fucose, D-Mannose, D-Glucose, sphingomyelin(SM), and soluble phospholipase A2 (sPLA2) inhibitors (dibucain, quinacrine). Accordingly, HLSchemico-physical properties (alkaline medium, high termostability, Ca2+-dependence, trypsintreatment, protease inhibitors) and SEM observations of the affected targets suggested that sPLA2could be responsible for changes and large alterations of the target cell membrane. An apoptoticactivity, as recorded by a caspase 3, 7 assay, was found by treating K562 cells with very dilutedHLS. A lytic mechanism involving sPLA2 and lectins promptly released by URGs and morula cellsrespectively is suggested, whereas target cell membrane SM could be a modulator of the enzymeactivity.",
author = "Matteo Cammarata and Aiti Vizzini and Nicolo' Parrinello and Giaramita, {Francesca Tiziana} and Mirella Vazzana and Vincenzo Arizza and Daniela Parrinello and Margherita Pergolizzi",
year = "2007",
language = "English",
volume = "329",
pages = "379--390",
journal = "Cell and Tissue Research",
issn = "0302-766X",
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TY - JOUR

T1 - Inducible lectins with galectin properties and human IL1 alpha epitopes opsonize yeast during the inflammatory response of the ascidian Ciona intestinalis

AU - Cammarata, Matteo

AU - Vizzini, Aiti

AU - Parrinello, Nicolo'

AU - Giaramita, Francesca Tiziana

AU - Vazzana, Mirella

AU - Arizza, Vincenzo

AU - Parrinello, Daniela

AU - Pergolizzi, Margherita

PY - 2007

Y1 - 2007

N2 - Hemocytes from the ascidian Ciona intestinalis exert in vitro Ca2+-dependent cytotoxic activitytoward mammalian erythrocytes and K562 cells. To examine the lytic mechanism, hemocytepopulations were separated (B1-B6 bands) through a Percoll discontinuous density gradient, thehemocyte cytotoxic activity (HCA) and the lytic activity of the hemocyte lysate supernatant (HLS)were assayed. In addition the separated hemocytes were cultured and the cell free medium (CFM)assayed after 3h culture. Results support that unilocular refractile hemocytes (URGs), enriched inB5, are cytotoxic. The B5-HLS contains lysins and the activity of B5-CFM shows that lysisns canbe released into a culture medium. The B5 activity was blocked by D-Galactose, α-Lactose,Lactulose, LacNAc, thiodigalactoside (TDG), L-Fucose, D-Mannose, D-Glucose, sphingomyelin(SM), and soluble phospholipase A2 (sPLA2) inhibitors (dibucain, quinacrine). Accordingly, HLSchemico-physical properties (alkaline medium, high termostability, Ca2+-dependence, trypsintreatment, protease inhibitors) and SEM observations of the affected targets suggested that sPLA2could be responsible for changes and large alterations of the target cell membrane. An apoptoticactivity, as recorded by a caspase 3, 7 assay, was found by treating K562 cells with very dilutedHLS. A lytic mechanism involving sPLA2 and lectins promptly released by URGs and morula cellsrespectively is suggested, whereas target cell membrane SM could be a modulator of the enzymeactivity.

AB - Hemocytes from the ascidian Ciona intestinalis exert in vitro Ca2+-dependent cytotoxic activitytoward mammalian erythrocytes and K562 cells. To examine the lytic mechanism, hemocytepopulations were separated (B1-B6 bands) through a Percoll discontinuous density gradient, thehemocyte cytotoxic activity (HCA) and the lytic activity of the hemocyte lysate supernatant (HLS)were assayed. In addition the separated hemocytes were cultured and the cell free medium (CFM)assayed after 3h culture. Results support that unilocular refractile hemocytes (URGs), enriched inB5, are cytotoxic. The B5-HLS contains lysins and the activity of B5-CFM shows that lysisns canbe released into a culture medium. The B5 activity was blocked by D-Galactose, α-Lactose,Lactulose, LacNAc, thiodigalactoside (TDG), L-Fucose, D-Mannose, D-Glucose, sphingomyelin(SM), and soluble phospholipase A2 (sPLA2) inhibitors (dibucain, quinacrine). Accordingly, HLSchemico-physical properties (alkaline medium, high termostability, Ca2+-dependence, trypsintreatment, protease inhibitors) and SEM observations of the affected targets suggested that sPLA2could be responsible for changes and large alterations of the target cell membrane. An apoptoticactivity, as recorded by a caspase 3, 7 assay, was found by treating K562 cells with very dilutedHLS. A lytic mechanism involving sPLA2 and lectins promptly released by URGs and morula cellsrespectively is suggested, whereas target cell membrane SM could be a modulator of the enzymeactivity.

UR - http://hdl.handle.net/10447/24890

M3 - Article

VL - 329

SP - 379

EP - 390

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

ER -