Inclusion of Trehalose (TRH) into liposomes to regulate uptake of this cryoprotectant into human hepatocytes.

Risultato della ricerca: Paper

Abstract

Purpose. Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by improving the efficiency of their cryopreservation. TRH-loaded liposomes could enhance epatocyte viability by regulating the intracellular uptake of the cryoprotectant [1]. Methods. TRH-loaded liposomes were prepared by film hydration method (EPC, sodium cholate, 200mM TRH solution) [2]. Liposomal size was determined by dynamic light scattering. Intraliposomal TRH content was measured using the Megazyme spectrophotometric method. After incubation (1-5 h, 37°C, 5% CO2, 95% RH) of human hepatocytes with TRH-loaded liposomes or equivalent TRH solution, intracellular sugar content was determined. Cell viability was evaluated by trypan blue exclusion. Results. Size analysis (320.2 nm diameter) and distribution (93.40.4%) suggested formation of highly homogeneous, reproducible Small Unilamellar Vesicles (SUVs). Encapsulation efficiency was 332% w/w. After incubation of hepatocytes with loaded liposomes, intracellular TRH content was about 0.1 mg/million of cells and the cell viability (858%) was unvarying and comparable to the control. By contrast, after incubation with TRH solution, hepatocyte viability dropped to 485%. Conclusions. TRH-loaded SUVs seem a useful tool in controlling intracellular uptake of the cryoprotectant. [1] Katenz E. et al., Liver Tranplantation 45 (2007) 13-38. [2] Chiantia S. et al., Langmuir 21 (2005) 4108-4116.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2009

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Trehalose
Liposomes
Hepatocytes
Unilamellar Liposomes
Cell Survival
Sodium Cholate
Trypan Blue
Cryopreservation
Cell Transplantation
Liver

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@conference{e2f04710c49d407c91608f74026b8f42,
title = "Inclusion of Trehalose (TRH) into liposomes to regulate uptake of this cryoprotectant into human hepatocytes.",
abstract = "Purpose. Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by improving the efficiency of their cryopreservation. TRH-loaded liposomes could enhance epatocyte viability by regulating the intracellular uptake of the cryoprotectant [1]. Methods. TRH-loaded liposomes were prepared by film hydration method (EPC, sodium cholate, 200mM TRH solution) [2]. Liposomal size was determined by dynamic light scattering. Intraliposomal TRH content was measured using the Megazyme spectrophotometric method. After incubation (1-5 h, 37°C, 5{\%} CO2, 95{\%} RH) of human hepatocytes with TRH-loaded liposomes or equivalent TRH solution, intracellular sugar content was determined. Cell viability was evaluated by trypan blue exclusion. Results. Size analysis (320.2 nm diameter) and distribution (93.40.4{\%}) suggested formation of highly homogeneous, reproducible Small Unilamellar Vesicles (SUVs). Encapsulation efficiency was 332{\%} w/w. After incubation of hepatocytes with loaded liposomes, intracellular TRH content was about 0.1 mg/million of cells and the cell viability (858{\%}) was unvarying and comparable to the control. By contrast, after incubation with TRH solution, hepatocyte viability dropped to 485{\%}. Conclusions. TRH-loaded SUVs seem a useful tool in controlling intracellular uptake of the cryoprotectant. [1] Katenz E. et al., Liver Tranplantation 45 (2007) 13-38. [2] Chiantia S. et al., Langmuir 21 (2005) 4108-4116.",
keywords = "Liposomes; Nanoscience; Cell cultures",
author = "{De Caro}, Viviana and Giulia Giandalia",
year = "2009",
language = "English",

}

TY - CONF

T1 - Inclusion of Trehalose (TRH) into liposomes to regulate uptake of this cryoprotectant into human hepatocytes.

AU - De Caro, Viviana

AU - Giandalia, Giulia

PY - 2009

Y1 - 2009

N2 - Purpose. Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by improving the efficiency of their cryopreservation. TRH-loaded liposomes could enhance epatocyte viability by regulating the intracellular uptake of the cryoprotectant [1]. Methods. TRH-loaded liposomes were prepared by film hydration method (EPC, sodium cholate, 200mM TRH solution) [2]. Liposomal size was determined by dynamic light scattering. Intraliposomal TRH content was measured using the Megazyme spectrophotometric method. After incubation (1-5 h, 37°C, 5% CO2, 95% RH) of human hepatocytes with TRH-loaded liposomes or equivalent TRH solution, intracellular sugar content was determined. Cell viability was evaluated by trypan blue exclusion. Results. Size analysis (320.2 nm diameter) and distribution (93.40.4%) suggested formation of highly homogeneous, reproducible Small Unilamellar Vesicles (SUVs). Encapsulation efficiency was 332% w/w. After incubation of hepatocytes with loaded liposomes, intracellular TRH content was about 0.1 mg/million of cells and the cell viability (858%) was unvarying and comparable to the control. By contrast, after incubation with TRH solution, hepatocyte viability dropped to 485%. Conclusions. TRH-loaded SUVs seem a useful tool in controlling intracellular uptake of the cryoprotectant. [1] Katenz E. et al., Liver Tranplantation 45 (2007) 13-38. [2] Chiantia S. et al., Langmuir 21 (2005) 4108-4116.

AB - Purpose. Problems with the limited availability of human hepatocytes for cell transplantation may be overcome by improving the efficiency of their cryopreservation. TRH-loaded liposomes could enhance epatocyte viability by regulating the intracellular uptake of the cryoprotectant [1]. Methods. TRH-loaded liposomes were prepared by film hydration method (EPC, sodium cholate, 200mM TRH solution) [2]. Liposomal size was determined by dynamic light scattering. Intraliposomal TRH content was measured using the Megazyme spectrophotometric method. After incubation (1-5 h, 37°C, 5% CO2, 95% RH) of human hepatocytes with TRH-loaded liposomes or equivalent TRH solution, intracellular sugar content was determined. Cell viability was evaluated by trypan blue exclusion. Results. Size analysis (320.2 nm diameter) and distribution (93.40.4%) suggested formation of highly homogeneous, reproducible Small Unilamellar Vesicles (SUVs). Encapsulation efficiency was 332% w/w. After incubation of hepatocytes with loaded liposomes, intracellular TRH content was about 0.1 mg/million of cells and the cell viability (858%) was unvarying and comparable to the control. By contrast, after incubation with TRH solution, hepatocyte viability dropped to 485%. Conclusions. TRH-loaded SUVs seem a useful tool in controlling intracellular uptake of the cryoprotectant. [1] Katenz E. et al., Liver Tranplantation 45 (2007) 13-38. [2] Chiantia S. et al., Langmuir 21 (2005) 4108-4116.

KW - Liposomes; Nanoscience; Cell cultures

UR - http://hdl.handle.net/10447/42627

M3 - Paper

ER -