In vitro evaluation of the antibacterial activity of cured dentin/enamel adhesive incorporating the antimicrobial agent MDPB

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Abstract

The aim of this study was to investigate in vitro the antibacterial effect of a dentin/enamel adhesive containing MDPB (Clearfil Protect Bond) after curing and to compare it with an adhesive not containing MDPB (Clearfil SE Bond). The antibacterial effect of the cured adhesives was tested on Enterococcus faecalis by the evaluation of growth inhibition in a 48-h microplate direct contact test (DCT). The presence of antibacterial activity due to soluble components was also investigated by an agar diffusion test (ADT) performed with the adhesives cured on the surface of dentin disks. In the DCT, both the tested materials showed complete inhibition of bacterial growth until the 7-h reading, regardless of whether direct contact with the bacterial inoculum was continued or limited to 1 h. Stationary growth phase turbidity levels were observed at 24 or 48-h incubation.The ADT produced no inhibition areas around the dentin disks. Both adhesives tested in vitro under conditions simulating those encountered in the restorative treatment of dental caries were equally able to delay bacterial growth demonstrating a similar bacteriostatic effect. As suggested by both the DCT and the ADT, the antibacterial effect of the two adhesives depends on direct contact and does not seem to be related to the diffusion of soluble components.
Lingua originaleEnglish
pagine (da-a)385-390
Numero di pagine6
RivistaNew Microbiologica
Volume32
Stato di pubblicazionePublished - 2009

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Dentin
Dental Enamel
Anti-Infective Agents
Adhesives
Agar
Growth
Enterococcus faecalis
Dental Caries
In Vitro Techniques
Reading

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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title = "In vitro evaluation of the antibacterial activity of cured dentin/enamel adhesive incorporating the antimicrobial agent MDPB",
abstract = "The aim of this study was to investigate in vitro the antibacterial effect of a dentin/enamel adhesive containing MDPB (Clearfil Protect Bond) after curing and to compare it with an adhesive not containing MDPB (Clearfil SE Bond). The antibacterial effect of the cured adhesives was tested on Enterococcus faecalis by the evaluation of growth inhibition in a 48-h microplate direct contact test (DCT). The presence of antibacterial activity due to soluble components was also investigated by an agar diffusion test (ADT) performed with the adhesives cured on the surface of dentin disks. In the DCT, both the tested materials showed complete inhibition of bacterial growth until the 7-h reading, regardless of whether direct contact with the bacterial inoculum was continued or limited to 1 h. Stationary growth phase turbidity levels were observed at 24 or 48-h incubation.The ADT produced no inhibition areas around the dentin disks. Both adhesives tested in vitro under conditions simulating those encountered in the restorative treatment of dental caries were equally able to delay bacterial growth demonstrating a similar bacteriostatic effect. As suggested by both the DCT and the ADT, the antibacterial effect of the two adhesives depends on direct contact and does not seem to be related to the diffusion of soluble components.",
keywords = "Dentin/enamel adhesives, MDP, MDPB, agar diffusion test, antibacterial activity, direct contact test",
author = "Giuseppe Gallina and Giovanni Giammanco and Cumbo, {Enzo Maria Giuseppe} and Giuseppe Pizzo and Caterina Mammina and Andrea Luciani",
year = "2009",
language = "English",
volume = "32",
pages = "385--390",
journal = "New Microbiologica",
issn = "1121-7138",
publisher = "Luigi Ponzio e figlio Editori",

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TY - JOUR

T1 - In vitro evaluation of the antibacterial activity of cured dentin/enamel adhesive incorporating the antimicrobial agent MDPB

AU - Gallina, Giuseppe

AU - Giammanco, Giovanni

AU - Cumbo, Enzo Maria Giuseppe

AU - Pizzo, Giuseppe

AU - Mammina, Caterina

AU - Luciani, Andrea

PY - 2009

Y1 - 2009

N2 - The aim of this study was to investigate in vitro the antibacterial effect of a dentin/enamel adhesive containing MDPB (Clearfil Protect Bond) after curing and to compare it with an adhesive not containing MDPB (Clearfil SE Bond). The antibacterial effect of the cured adhesives was tested on Enterococcus faecalis by the evaluation of growth inhibition in a 48-h microplate direct contact test (DCT). The presence of antibacterial activity due to soluble components was also investigated by an agar diffusion test (ADT) performed with the adhesives cured on the surface of dentin disks. In the DCT, both the tested materials showed complete inhibition of bacterial growth until the 7-h reading, regardless of whether direct contact with the bacterial inoculum was continued or limited to 1 h. Stationary growth phase turbidity levels were observed at 24 or 48-h incubation.The ADT produced no inhibition areas around the dentin disks. Both adhesives tested in vitro under conditions simulating those encountered in the restorative treatment of dental caries were equally able to delay bacterial growth demonstrating a similar bacteriostatic effect. As suggested by both the DCT and the ADT, the antibacterial effect of the two adhesives depends on direct contact and does not seem to be related to the diffusion of soluble components.

AB - The aim of this study was to investigate in vitro the antibacterial effect of a dentin/enamel adhesive containing MDPB (Clearfil Protect Bond) after curing and to compare it with an adhesive not containing MDPB (Clearfil SE Bond). The antibacterial effect of the cured adhesives was tested on Enterococcus faecalis by the evaluation of growth inhibition in a 48-h microplate direct contact test (DCT). The presence of antibacterial activity due to soluble components was also investigated by an agar diffusion test (ADT) performed with the adhesives cured on the surface of dentin disks. In the DCT, both the tested materials showed complete inhibition of bacterial growth until the 7-h reading, regardless of whether direct contact with the bacterial inoculum was continued or limited to 1 h. Stationary growth phase turbidity levels were observed at 24 or 48-h incubation.The ADT produced no inhibition areas around the dentin disks. Both adhesives tested in vitro under conditions simulating those encountered in the restorative treatment of dental caries were equally able to delay bacterial growth demonstrating a similar bacteriostatic effect. As suggested by both the DCT and the ADT, the antibacterial effect of the two adhesives depends on direct contact and does not seem to be related to the diffusion of soluble components.

KW - Dentin/enamel adhesives

KW - MDP

KW - MDPB

KW - agar diffusion test

KW - antibacterial activity

KW - direct contact test

UR - http://hdl.handle.net/10447/42501

UR - http://www.microbiologica.net/

M3 - Article

VL - 32

SP - 385

EP - 390

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

ER -