In vitro anther culture of sweet orange (Citrus sinensis L. Osbeck) genotypes and of a C. clementina x C. sinensis ‘Hamlin’ hybrid.

Maria Germana', Rodrigo R. Latado, Adriana P. Martinelli, Jean C. Cardoso

Risultato della ricerca: Articlepeer review

12 Citazioni (Scopus)

Abstract

Citrus, and particularly sweet oranges, are very recalcitrant to anther culture. In this paper it was evaluated for the first time the response of 27 genotypes of Citrus sinensis and of one hybrid C. clementina x C. sinensis, to in vitro anther culture. Ten genotypes of sweet oranges showed embryogenic callus induction, mostly blood sweet oranges genotypes, such as Tarocco, Moro and Sanguinelli. In vitro microspore developmental switches from the gamethophytic to the sporophytic pathway were shown by DAPI staining in microspores of these responsive genotypes, after 10 months in culture. However, microsatellite marker analyses showed that these calli were heterozygous. The flow-cytometric analysis of these embryogenic calli showed the presence of two peaks, corresponding to haploid (n) and diploid (2n) genotypes. Differently, anther cultures of the hybrid C. clementina x C. sinensis produced tri-haploid (3n) embryogenic calli and the embryos obtained were homozygous when analyzed by molecular markers (sample sequence repeats), confirming the more responsive characteristic of clementine to microspore embryogenesis through anther culture.
Lingua originaleEnglish
pagine (da-a)455-464
Numero di pagine10
RivistaPLANT CELL TISSUE AND ORGAN CULTURE
Volume117
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

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