In Vitro–In Vivo Fluctuation Spectroscopies

Massimo Panzica

Risultato della ricerca: Chapter

3 Citazioni (Scopus)

Abstract

Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.
Lingua originaleEnglish
Titolo della pubblicazione ospiteOPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension
Pagine165-181
Numero di pagine17
Stato di pubblicazionePublished - 2011
Pubblicato esternamenteYes

Fingerprint

Spectroscopy
Fluorescence
Cell membranes
Coloring Agents
Photons
Scattering
Nanoparticles
Molecules

Cita questo

Panzica, M. (2011). In Vitro–In Vivo Fluctuation Spectroscopies. In OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension (pagg. 165-181)

In Vitro–In Vivo Fluctuation Spectroscopies. / Panzica, Massimo.

OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension. 2011. pag. 165-181.

Risultato della ricerca: Chapter

Panzica, M 2011, In Vitro–In Vivo Fluctuation Spectroscopies. in OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension. pagg. 165-181.
Panzica M. In Vitro–In Vivo Fluctuation Spectroscopies. In OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension. 2011. pag. 165-181
Panzica, Massimo. / In Vitro–In Vivo Fluctuation Spectroscopies. OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension. 2011. pagg. 165-181
@inbook{d78c50a910524f33b46a6896e50e86d5,
title = "In Vitro–In Vivo Fluctuation Spectroscopies",
abstract = "Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.",
keywords = "FCS, FFS, Fluorescence Correlation Spectroscopy, Spectroscopies, cell, fluctuation, nanoparticle., single molecule, vitro, vivo",
author = "Massimo Panzica",
year = "2011",
language = "English",
isbn = "978-3-642-15174-3",
pages = "165--181",
booktitle = "OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension",

}

TY - CHAP

T1 - In Vitro–In Vivo Fluctuation Spectroscopies

AU - Panzica, Massimo

PY - 2011

Y1 - 2011

N2 - Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.

AB - Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.

KW - FCS

KW - FFS

KW - Fluorescence Correlation Spectroscopy

KW - Spectroscopies

KW - cell

KW - fluctuation

KW - nanoparticle.

KW - single molecule

KW - vitro

KW - vivo

UR - http://hdl.handle.net/10447/52422

UR - http://www.springerlink.com/content/kw281h6673342202/

M3 - Chapter

SN - 978-3-642-15174-3

SP - 165

EP - 181

BT - OPTICAL FLUORESCENCE MICROSCOPY - From the Spectral to the Nano Dimension

ER -