In situ crosslinkable hyaluronan hydrogels for tissue engineering

Fabio Salvatore Palumbo, Glenn D. Prestwich, Yi Luo, Fabio S. Palumbo, Yanchun Liu, Xiao Zheng Shu

Risultato della ricerca: Article

470 Citazioni (Scopus)

Abstract

We describe the development of an injectable, cell-containing hydrogel that supports cell proliferation and growth to permit in vivo engineering of new tissues. Two thiolated hyaluronan (HA) derivatives were coupled to four α,β-unsaturated ester and amide derivatives of poly(ethylene glycol) (PEG) 3400. The relative chemical reactivity with cysteine decreased in the order PEG-diacrylate (PEGDA)⪢PEG-dimethacrylate>PEG-diacrylamide>PEG-dimethacrylamide. The 3-thiopropanoyl hydrazide derivative (HA-DTPH) was more reactive than the 4-thiobutanoyl hydrazide, HA-DTBH. The crosslinking of HA-DTPH with PEGDA in a molar ratio of 2:1 occurred in approximately 9 min, suitable for an in situ crosslinking applications. The in vitro cytocompatibility and in vivo biocompatibility were evaluated using T31 human tracheal scar fibroblasts, which were suspended in medium in HA-DTPH prior to addition of the PEGDA solution. The majority of cells survived crosslinking and the cell density increased tenfold during the 4-week culture period in vitro. Cell-loaded hydrogels were also implanted subcutaneously in the flanks of nude mice, and after immunohistochemistry showed that the encapsulated cells retained the fibroblast phenotype and secreted extracellular matrix in vivo. These results confirm the potential utility of the HA-DTPH-PEGDA hydrogel as an in situ crosslinkable, injectable material for tissue engineering.
Lingua originaleEnglish
pagine (da-a)1339-1348
Numero di pagine10
RivistaBiomaterials
Volume25(7-8)
Stato di pubblicazionePublished - 2004

Fingerprint

Hydrogels
Hyaluronic Acid
Tissue Engineering
Tissue engineering
Polyethylene glycols
Hydrogel
Crosslinking
Fibroblasts
Derivatives
Injections
Ethylene Glycol
Nude Mice
Amides
Cicatrix
Extracellular Matrix
Cysteine
Chemical reactivity
Esters
Cell Count
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cita questo

Palumbo, F. S., Prestwich, G. D., Luo, Y., Palumbo, F. S., Liu, Y., & Shu, X. Z. (2004). In situ crosslinkable hyaluronan hydrogels for tissue engineering. Biomaterials, 25(7-8), 1339-1348.

In situ crosslinkable hyaluronan hydrogels for tissue engineering. / Palumbo, Fabio Salvatore; Prestwich, Glenn D.; Luo, Yi; Palumbo, Fabio S.; Liu, Yanchun; Shu, Xiao Zheng.

In: Biomaterials, Vol. 25(7-8), 2004, pag. 1339-1348.

Risultato della ricerca: Article

Palumbo, FS, Prestwich, GD, Luo, Y, Palumbo, FS, Liu, Y & Shu, XZ 2004, 'In situ crosslinkable hyaluronan hydrogels for tissue engineering', Biomaterials, vol. 25(7-8), pagg. 1339-1348.
Palumbo FS, Prestwich GD, Luo Y, Palumbo FS, Liu Y, Shu XZ. In situ crosslinkable hyaluronan hydrogels for tissue engineering. Biomaterials. 2004;25(7-8):1339-1348.
Palumbo, Fabio Salvatore ; Prestwich, Glenn D. ; Luo, Yi ; Palumbo, Fabio S. ; Liu, Yanchun ; Shu, Xiao Zheng. / In situ crosslinkable hyaluronan hydrogels for tissue engineering. In: Biomaterials. 2004 ; Vol. 25(7-8). pagg. 1339-1348.
@article{8e1543690cc34273a32e608f028f07f5,
title = "In situ crosslinkable hyaluronan hydrogels for tissue engineering",
abstract = "We describe the development of an injectable, cell-containing hydrogel that supports cell proliferation and growth to permit in vivo engineering of new tissues. Two thiolated hyaluronan (HA) derivatives were coupled to four α,β-unsaturated ester and amide derivatives of poly(ethylene glycol) (PEG) 3400. The relative chemical reactivity with cysteine decreased in the order PEG-diacrylate (PEGDA)⪢PEG-dimethacrylate>PEG-diacrylamide>PEG-dimethacrylamide. The 3-thiopropanoyl hydrazide derivative (HA-DTPH) was more reactive than the 4-thiobutanoyl hydrazide, HA-DTBH. The crosslinking of HA-DTPH with PEGDA in a molar ratio of 2:1 occurred in approximately 9 min, suitable for an in situ crosslinking applications. The in vitro cytocompatibility and in vivo biocompatibility were evaluated using T31 human tracheal scar fibroblasts, which were suspended in medium in HA-DTPH prior to addition of the PEGDA solution. The majority of cells survived crosslinking and the cell density increased tenfold during the 4-week culture period in vitro. Cell-loaded hydrogels were also implanted subcutaneously in the flanks of nude mice, and after immunohistochemistry showed that the encapsulated cells retained the fibroblast phenotype and secreted extracellular matrix in vivo. These results confirm the potential utility of the HA-DTPH-PEGDA hydrogel as an in situ crosslinkable, injectable material for tissue engineering.",
keywords = "Cell encapsulation, Cell-compatible crosslinking, Diacrylates, Glycosaminoglycan, In vivo biocompatibility, Polyethylene glycol",
author = "Palumbo, {Fabio Salvatore} and Prestwich, {Glenn D.} and Yi Luo and Palumbo, {Fabio S.} and Yanchun Liu and Shu, {Xiao Zheng}",
year = "2004",
language = "English",
volume = "25(7-8)",
pages = "1339--1348",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - In situ crosslinkable hyaluronan hydrogels for tissue engineering

AU - Palumbo, Fabio Salvatore

AU - Prestwich, Glenn D.

AU - Luo, Yi

AU - Palumbo, Fabio S.

AU - Liu, Yanchun

AU - Shu, Xiao Zheng

PY - 2004

Y1 - 2004

N2 - We describe the development of an injectable, cell-containing hydrogel that supports cell proliferation and growth to permit in vivo engineering of new tissues. Two thiolated hyaluronan (HA) derivatives were coupled to four α,β-unsaturated ester and amide derivatives of poly(ethylene glycol) (PEG) 3400. The relative chemical reactivity with cysteine decreased in the order PEG-diacrylate (PEGDA)⪢PEG-dimethacrylate>PEG-diacrylamide>PEG-dimethacrylamide. The 3-thiopropanoyl hydrazide derivative (HA-DTPH) was more reactive than the 4-thiobutanoyl hydrazide, HA-DTBH. The crosslinking of HA-DTPH with PEGDA in a molar ratio of 2:1 occurred in approximately 9 min, suitable for an in situ crosslinking applications. The in vitro cytocompatibility and in vivo biocompatibility were evaluated using T31 human tracheal scar fibroblasts, which were suspended in medium in HA-DTPH prior to addition of the PEGDA solution. The majority of cells survived crosslinking and the cell density increased tenfold during the 4-week culture period in vitro. Cell-loaded hydrogels were also implanted subcutaneously in the flanks of nude mice, and after immunohistochemistry showed that the encapsulated cells retained the fibroblast phenotype and secreted extracellular matrix in vivo. These results confirm the potential utility of the HA-DTPH-PEGDA hydrogel as an in situ crosslinkable, injectable material for tissue engineering.

AB - We describe the development of an injectable, cell-containing hydrogel that supports cell proliferation and growth to permit in vivo engineering of new tissues. Two thiolated hyaluronan (HA) derivatives were coupled to four α,β-unsaturated ester and amide derivatives of poly(ethylene glycol) (PEG) 3400. The relative chemical reactivity with cysteine decreased in the order PEG-diacrylate (PEGDA)⪢PEG-dimethacrylate>PEG-diacrylamide>PEG-dimethacrylamide. The 3-thiopropanoyl hydrazide derivative (HA-DTPH) was more reactive than the 4-thiobutanoyl hydrazide, HA-DTBH. The crosslinking of HA-DTPH with PEGDA in a molar ratio of 2:1 occurred in approximately 9 min, suitable for an in situ crosslinking applications. The in vitro cytocompatibility and in vivo biocompatibility were evaluated using T31 human tracheal scar fibroblasts, which were suspended in medium in HA-DTPH prior to addition of the PEGDA solution. The majority of cells survived crosslinking and the cell density increased tenfold during the 4-week culture period in vitro. Cell-loaded hydrogels were also implanted subcutaneously in the flanks of nude mice, and after immunohistochemistry showed that the encapsulated cells retained the fibroblast phenotype and secreted extracellular matrix in vivo. These results confirm the potential utility of the HA-DTPH-PEGDA hydrogel as an in situ crosslinkable, injectable material for tissue engineering.

KW - Cell encapsulation

KW - Cell-compatible crosslinking

KW - Diacrylates

KW - Glycosaminoglycan

KW - In vivo biocompatibility

KW - Polyethylene glycol

UR - http://hdl.handle.net/10447/26695

M3 - Article

VL - 25(7-8)

SP - 1339

EP - 1348

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -