Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defect repair

Vincenzo La Carrubba, Ilenia Vitrano, Francesco Carfi' Pavia, Gioacchino Conoscenti, Valerio Maria Bartolo Brucato, Daniele Bellavia, Angela De Luca, Valeria Carina, Viviana Costa, Gianluca Giavaresi, Lavinia Raimondi, Angela De Luca, Daniele Bellavia, Lavinia Raimondi, Valeria Carina

Risultato della ricerca: Articlepeer review

10 Citazioni (Scopus)

Abstract

Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic (d3D) 3D culture conditions at 7 and 21 days. The colonization rate of hMSCs and osteogenic differentiation were amplified by d3D when physical stimulation was provided by a perfusion bioreactor. Increase in dsDNA content (p < 0.0005), up- regulation of RUNX2, ALPL, SPP1 (p < 0.0005) and SOX9 (p < 0.005) gene expression, and more calcium nodule forma- tion (p < 0.0005) were observed in d3D cultures in comparison to s3D ones over time. Dynamic 3D culture, mimicking the mechanical signals of bone environment, improved significantly osteogenic differentiation of hMSCs on PLLA/nHA scaffold, without the addition of growth factors, confirming this composite scaffold suitable for bone regeneration
Lingua originaleEnglish
pagine (da-a)250-257
Numero di pagine8
RivistaJournal of Bioscience and Bioengineering
Volume129
Stato di pubblicazionePublished - 2020

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1500.1502???
  • ???subjectarea.asjc.2400.2402???

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