Improved micropropagation in Polygala myrtifolia

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Abstract

Stem segments from apical shoot tips of Polygala myrtifolia were used as primary explants to establish in vitro cultures. Axillary shoots produced on noncontaminated explants were excised and recultured in the same medium to increase the stock of shoot cultures. Equal molar concentrations of five cytokinins 2-isopentenyladenine (2iP), kinetin, zeatin, N6-benzyladenine (BA) and adenine were tested for ability to induce axillary shoot development from double node stem segments. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog agar medium supplemented with 1.8 M BA. Seven indole-3-acetic acid (IAA) concentrations (2.8, 5.7, 8.6, 11.4, 14.3, 17.1 M) were tested to determine the optimum conditions for in vitro rooting of microshoots. Up to 72 % of the microshoots rooted with 14.3 M IAA. Other auxins tested, -naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA), were less effective than IAA in inducing adventitious root formation. All rooted plantlets having more than 3 roots were successfully established in soil.
Lingua originaleEnglish
pagine (da-a)86-89
Numero di pagine4
RivistaIN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL
Volume40
Stato di pubblicazionePublished - 2004

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Polygala
micropropagation
indole acetic acid
shoots
benzyladenine
explants
stem nodes
isopentenyladenine
adventitious roots
zeatin
adenine
indole butyric acid
kinetin
naphthaleneacetic acid
in vitro culture
cytokinins
plantlets
rooting
auxins
agar

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Cell Biology
  • Developmental Biology
  • Plant Science

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title = "Improved micropropagation in Polygala myrtifolia",
abstract = "Stem segments from apical shoot tips of Polygala myrtifolia were used as primary explants to establish in vitro cultures. Axillary shoots produced on noncontaminated explants were excised and recultured in the same medium to increase the stock of shoot cultures. Equal molar concentrations of five cytokinins 2-isopentenyladenine (2iP), kinetin, zeatin, N6-benzyladenine (BA) and adenine were tested for ability to induce axillary shoot development from double node stem segments. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog agar medium supplemented with 1.8 M BA. Seven indole-3-acetic acid (IAA) concentrations (2.8, 5.7, 8.6, 11.4, 14.3, 17.1 M) were tested to determine the optimum conditions for in vitro rooting of microshoots. Up to 72 {\%} of the microshoots rooted with 14.3 M IAA. Other auxins tested, -naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA), were less effective than IAA in inducing adventitious root formation. All rooted plantlets having more than 3 roots were successfully established in soil.",
author = "Giovanni Iapichino",
year = "2004",
language = "English",
volume = "40",
pages = "86--89",
journal = "In Vitro Cellular and Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer New York",

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TY - JOUR

T1 - Improved micropropagation in Polygala myrtifolia

AU - Iapichino, Giovanni

PY - 2004

Y1 - 2004

N2 - Stem segments from apical shoot tips of Polygala myrtifolia were used as primary explants to establish in vitro cultures. Axillary shoots produced on noncontaminated explants were excised and recultured in the same medium to increase the stock of shoot cultures. Equal molar concentrations of five cytokinins 2-isopentenyladenine (2iP), kinetin, zeatin, N6-benzyladenine (BA) and adenine were tested for ability to induce axillary shoot development from double node stem segments. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog agar medium supplemented with 1.8 M BA. Seven indole-3-acetic acid (IAA) concentrations (2.8, 5.7, 8.6, 11.4, 14.3, 17.1 M) were tested to determine the optimum conditions for in vitro rooting of microshoots. Up to 72 % of the microshoots rooted with 14.3 M IAA. Other auxins tested, -naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA), were less effective than IAA in inducing adventitious root formation. All rooted plantlets having more than 3 roots were successfully established in soil.

AB - Stem segments from apical shoot tips of Polygala myrtifolia were used as primary explants to establish in vitro cultures. Axillary shoots produced on noncontaminated explants were excised and recultured in the same medium to increase the stock of shoot cultures. Equal molar concentrations of five cytokinins 2-isopentenyladenine (2iP), kinetin, zeatin, N6-benzyladenine (BA) and adenine were tested for ability to induce axillary shoot development from double node stem segments. The highest rate of axillary shoot proliferation was induced on Murashige and Skoog agar medium supplemented with 1.8 M BA. Seven indole-3-acetic acid (IAA) concentrations (2.8, 5.7, 8.6, 11.4, 14.3, 17.1 M) were tested to determine the optimum conditions for in vitro rooting of microshoots. Up to 72 % of the microshoots rooted with 14.3 M IAA. Other auxins tested, -naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA), were less effective than IAA in inducing adventitious root formation. All rooted plantlets having more than 3 roots were successfully established in soil.

UR - http://hdl.handle.net/10447/22428

M3 - Article

VL - 40

SP - 86

EP - 89

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

SN - 1071-2690

ER -