Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes

Monica Celi, Natoli, Annalisa Pinsino, Monica Celi, Di Bella, Natoli, Valeria Matranga, Annalisa Pinsino

Risultato della ricerca: Article

50 Citazioni (Scopus)

Abstract

Three specialized cell types constitute the heterogeneous population present in the coelomic cavity fluid of sea urchins. The list includes: phagocytes, which undergo a stress-induced petaloid-filopodial transition, white or red amoebocytes and vibratile cells. As a whole, they act as the immune defense system of the sea urchin and respond to environmental and experimental challenge triggering specific stress markers. Here we extended our studies on coelomocytes short-term cultures by describing the morphology and occurrence of each cell type and analyzing their response to UV-B radiation at the biochemical level and with respect to DNA damage. The effects of different doses, ranging from 500 to 2,000 J/m2, on cells, which were then cultured in vitro for 1-6 h were tested. As early as 1 h after irradiation we found an increase in the levels of the heat shock protein70 (hsp) stress marker. A peak at 1,000 J/m2 corresponding to a 3.4-fold hsp70 increase over the levels of control coelomocytes was observed. Concurrently, we found an increase in the number of apoptotic nuclei detected by Hoechst staining, which varied from 7.1 to 15.6% in cells that were exposed to 500 and 2,000 J/m2, respectively. On the basis of our findings we confirm sea urchin coelomocytes as a sensitive cell culture system for environmental studies and propose their use for the analysis on the effects of UV-B radiation.
Lingua originaleEnglish
pagine (da-a)25-34
Numero di pagine10
RivistaMarine Biology
Volume149
Stato di pubblicazionePublished - 2006

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Echinoidea
heat shock
cells
irradiation
cavity
fold
phagocytes
DNA
damage
fluid
DNA damage
heat stress
cell culture
sea
radiation
dosage
effect
marker

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Cita questo

Celi, M., Natoli, Pinsino, A., Celi, M., Di Bella, Natoli, ... Pinsino, A. (2006). Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes. Marine Biology, 149, 25-34.

Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes. / Celi, Monica; Natoli; Pinsino, Annalisa; Celi, Monica; Di Bella; Natoli; Matranga, Valeria; Pinsino, Annalisa.

In: Marine Biology, Vol. 149, 2006, pag. 25-34.

Risultato della ricerca: Article

Celi, M, Natoli, Pinsino, A, Celi, M, Di Bella, Natoli, Matranga, V & Pinsino, A 2006, 'Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes', Marine Biology, vol. 149, pagg. 25-34.
Celi M, Natoli, Pinsino A, Celi M, Di Bella, Natoli e altri. Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes. Marine Biology. 2006;149:25-34.
Celi, Monica ; Natoli ; Pinsino, Annalisa ; Celi, Monica ; Di Bella ; Natoli ; Matranga, Valeria ; Pinsino, Annalisa. / Impacts of UV-B radiation on short-term cultures of sea urchin coelomocytes. In: Marine Biology. 2006 ; Vol. 149. pagg. 25-34.
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abstract = "Three specialized cell types constitute the heterogeneous population present in the coelomic cavity fluid of sea urchins. The list includes: phagocytes, which undergo a stress-induced petaloid-filopodial transition, white or red amoebocytes and vibratile cells. As a whole, they act as the immune defense system of the sea urchin and respond to environmental and experimental challenge triggering specific stress markers. Here we extended our studies on coelomocytes short-term cultures by describing the morphology and occurrence of each cell type and analyzing their response to UV-B radiation at the biochemical level and with respect to DNA damage. The effects of different doses, ranging from 500 to 2,000 J/m2, on cells, which were then cultured in vitro for 1-6 h were tested. As early as 1 h after irradiation we found an increase in the levels of the heat shock protein70 (hsp) stress marker. A peak at 1,000 J/m2 corresponding to a 3.4-fold hsp70 increase over the levels of control coelomocytes was observed. Concurrently, we found an increase in the number of apoptotic nuclei detected by Hoechst staining, which varied from 7.1 to 15.6{\%} in cells that were exposed to 500 and 2,000 J/m2, respectively. On the basis of our findings we confirm sea urchin coelomocytes as a sensitive cell culture system for environmental studies and propose their use for the analysis on the effects of UV-B radiation.",
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AB - Three specialized cell types constitute the heterogeneous population present in the coelomic cavity fluid of sea urchins. The list includes: phagocytes, which undergo a stress-induced petaloid-filopodial transition, white or red amoebocytes and vibratile cells. As a whole, they act as the immune defense system of the sea urchin and respond to environmental and experimental challenge triggering specific stress markers. Here we extended our studies on coelomocytes short-term cultures by describing the morphology and occurrence of each cell type and analyzing their response to UV-B radiation at the biochemical level and with respect to DNA damage. The effects of different doses, ranging from 500 to 2,000 J/m2, on cells, which were then cultured in vitro for 1-6 h were tested. As early as 1 h after irradiation we found an increase in the levels of the heat shock protein70 (hsp) stress marker. A peak at 1,000 J/m2 corresponding to a 3.4-fold hsp70 increase over the levels of control coelomocytes was observed. Concurrently, we found an increase in the number of apoptotic nuclei detected by Hoechst staining, which varied from 7.1 to 15.6% in cells that were exposed to 500 and 2,000 J/m2, respectively. On the basis of our findings we confirm sea urchin coelomocytes as a sensitive cell culture system for environmental studies and propose their use for the analysis on the effects of UV-B radiation.

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