Immunomorphological Pattern of Molecular Chaperones in Normal and Pathological Thyroid Tissues and Circulating Exosomes: Potential Use in Clinics

Francesco Cappello, Rossana Porcasi, Giovanni Tomasello, Fabio Bucchieri, Francesca Rappa, Daniela Cabibi, Rosario Barone, Calogero Cipolla, Claudia Campanella, Giuseppa Graceffa, Celeste Caruso Bavisotto, Alessandro Pitruzzella, Antonella Marino Gammazza, Pier Luigi San Biagio, Celeste Caruso Bavisotto, Pier Luigi San Biagio, Fabio Bucchieri, Donatella Bulone, Everly Conway De Macario, Francesco CappelloAlberto J. L. Macario, Pier Luigi San Biagio

Risultato della ricerca: Articlepeer review

14 Citazioni (Scopus)

Abstract

The thyroid is a major component of the endocrine system and its pathology can cause serious diseases, e.g., papillary carcinoma (PC). However, the carcinogenic mechanisms are poorly understood and clinical useful biomarkers are scarce. Therefore, we determined if there are quantitative patterns of molecular chaperones in the tumor tissue and circulating exosomes that may be useful in diagnosis and provide clues on their participation in carcinogenesis. Hsp27, Hsp60, Hsp70, and Hsp90 were quantified by immunohistochemistry in PC, benign goiter (BG), and normal peritumoral tissue (PT). The same chaperones were assessed in plasma exosomes from PC and BG patients before and after ablative surgery, using Western blotting. Hsp27, Hsp60, and Hsp90 were increased in PC in comparison with PT and BG but no differences were found for Hsp70. Similarly, exosomal levels of Hsp27, Hsp60, and Hsp90 were higher in PC than in BG, and those in PC were higher before ablative surgery than after it. Hsp27, Hsp60, and Hsp90 show distinctive quantitative patterns in thyroid tissue and circulating exosomes in PC as compared with BG, suggesting some implication in the carcinogenesis of these chaperones and indicating their potential as biomarkers for clinical applications.
Lingua originaleEnglish
pagine (da-a)1-12
Numero di pagine12
RivistaInternational Journal of Molecular Sciences
Volume20
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1300.1312???
  • ???subjectarea.asjc.1600.1607???
  • ???subjectarea.asjc.1700.1706???
  • ???subjectarea.asjc.1600.1606???
  • ???subjectarea.asjc.1600.1605???
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