Identification of SNPs in the promoter of β-lactoglobulin gene in three Sicilian goat breeds

Baldassare Portolano, Maria Teresa Sardina, Emilio Scotti, Silvia Braglia

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Abstract

The aim of this work was to sequence the full-length promoter region of the caprineβ-lactoglobulin (β-lg) gene in three Sicilian goat breeds (Girgentana, Maltese, and Derivata di Siria),in order to identify polymorphisms, to search for transcription factors (TFs) sites, and to check if polymorphismsfound lay within TFs binding sites. The promoter region of β-lg gene in Sicilian goat breedsshowed high level of polymorphism due to the presence of 31 SNPs. Binding sites for several TFs werefound within the goat β-lg promoter and within regions conserved between ovine and caprine species.Two SNPs were detected within TFs binding sites, such as MPBF and NF-I. Further studies are inprogress to confirm polymorphic sites, to evaluate the possible effect of these mutations on bindingaffinity of TFs, their relationship with β-lg gene expression, and the functional role of SNPs within theTFs sites of the promoter region on milk traits
Lingua originaleEnglish
pagine (da-a)147-149
Numero di pagine3
RivistaItalian Journal of Animal Science
Volume8
Stato di pubblicazionePublished - 2009

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lactoglobulins
goat breeds
transcription factors
promoter regions
goats
binding sites
genes
genetic polymorphism
mutation
sheep
milk
gene expression

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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title = "Identification of SNPs in the promoter of β-lactoglobulin gene in three Sicilian goat breeds",
abstract = "The aim of this work was to sequence the full-length promoter region of the caprineβ-lactoglobulin (β-lg) gene in three Sicilian goat breeds (Girgentana, Maltese, and Derivata di Siria),in order to identify polymorphisms, to search for transcription factors (TFs) sites, and to check if polymorphismsfound lay within TFs binding sites. The promoter region of β-lg gene in Sicilian goat breedsshowed high level of polymorphism due to the presence of 31 SNPs. Binding sites for several TFs werefound within the goat β-lg promoter and within regions conserved between ovine and caprine species.Two SNPs were detected within TFs binding sites, such as MPBF and NF-I. Further studies are inprogress to confirm polymorphic sites, to evaluate the possible effect of these mutations on bindingaffinity of TFs, their relationship with β-lg gene expression, and the functional role of SNPs within theTFs sites of the promoter region on milk traits",
author = "Baldassare Portolano and Sardina, {Maria Teresa} and Emilio Scotti and Silvia Braglia",
year = "2009",
language = "English",
volume = "8",
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journal = "Italian Journal of Animal Science",
issn = "1594-4077",
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TY - JOUR

T1 - Identification of SNPs in the promoter of β-lactoglobulin gene in three Sicilian goat breeds

AU - Portolano, Baldassare

AU - Sardina, Maria Teresa

AU - Scotti, Emilio

AU - Braglia, Silvia

PY - 2009

Y1 - 2009

N2 - The aim of this work was to sequence the full-length promoter region of the caprineβ-lactoglobulin (β-lg) gene in three Sicilian goat breeds (Girgentana, Maltese, and Derivata di Siria),in order to identify polymorphisms, to search for transcription factors (TFs) sites, and to check if polymorphismsfound lay within TFs binding sites. The promoter region of β-lg gene in Sicilian goat breedsshowed high level of polymorphism due to the presence of 31 SNPs. Binding sites for several TFs werefound within the goat β-lg promoter and within regions conserved between ovine and caprine species.Two SNPs were detected within TFs binding sites, such as MPBF and NF-I. Further studies are inprogress to confirm polymorphic sites, to evaluate the possible effect of these mutations on bindingaffinity of TFs, their relationship with β-lg gene expression, and the functional role of SNPs within theTFs sites of the promoter region on milk traits

AB - The aim of this work was to sequence the full-length promoter region of the caprineβ-lactoglobulin (β-lg) gene in three Sicilian goat breeds (Girgentana, Maltese, and Derivata di Siria),in order to identify polymorphisms, to search for transcription factors (TFs) sites, and to check if polymorphismsfound lay within TFs binding sites. The promoter region of β-lg gene in Sicilian goat breedsshowed high level of polymorphism due to the presence of 31 SNPs. Binding sites for several TFs werefound within the goat β-lg promoter and within regions conserved between ovine and caprine species.Two SNPs were detected within TFs binding sites, such as MPBF and NF-I. Further studies are inprogress to confirm polymorphic sites, to evaluate the possible effect of these mutations on bindingaffinity of TFs, their relationship with β-lg gene expression, and the functional role of SNPs within theTFs sites of the promoter region on milk traits

UR - http://hdl.handle.net/10447/62447

M3 - Article

VL - 8

SP - 147

EP - 149

JO - Italian Journal of Animal Science

JF - Italian Journal of Animal Science

SN - 1594-4077

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