Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine

Riccardo Alessandro, Simona Fontana, Thomas Kislinger, O. John Semmes, Simona Fontana, Jeffrey A. Medin, Dean A. Troyer, Richard R. Drake, Julius O. Nyalwidhe, Vladimir Ignatchenko, Simona Principe, Raymond S. Lance, Yunee Kim

Risultato della ricerca: Article

33 Citazioni (Scopus)

Abstract

Urinary expressed prostatic secretion or “EPS-urine” is proximal tissue fluid that is collected after a digital rectal exam (DRE). EPS-urine is a rich source of prostatederived proteins that can be used for biomarker discovery for prostate cancer (PCa) and other prostatic diseases. We previously conducted a comprehensive proteome analysis of direct expressed prostatic secretions (EPS). In the current study, we defined the proteome of EPS-urine employing Multidimensional Protein Identification Technology (MudPIT) and providing a comprehensive catalogue of this body fluid for future biomarker studies. We identified 1022 unique proteins in a heterogeneous cohort of 11 EPS-urines derived from biopsy negative noncancer diagnoses with some benign prostatic diseases (BPH) and lowgrade PCa, representative of secreted prostate and immune system-derived proteins in a urine background. We further applied MudPIT-based proteomics to generate and compare the differential proteome from a subset of pooled urines (pre-DRE) and EPS-urines (post- DRE) from noncancer and PCa patients. The direct proteomic comparison of these highly controlled patient sample pools enabled us to define a list of prostate-enriched proteins detectable in EPS-urine and distinguishable from a complex urine protein background. A combinatorial analysis of both proteomics data sets and systematic integration with publicly available proteomics data of related body fluids, human tissue transcriptomic data, and immunohistochemistry images from the Human Protein Atlas database allowed us to demarcate a robust panel of 49 prostate-derived proteins in EPS-urine. Finally, we validated the expression of seven of these proteins using Western blotting, supporting the likelihood that they originate from the prostate. The definition of these prostatic proteins in EPS-urine samples provides a reference for future investigations for prostatic-disease biomarker studies.
Lingua originaleEnglish
pagine (da-a)2386-2396
Numero di pagine11
RivistaJournal of Proteome Research
Volume11
Stato di pubblicazionePublished - 2012

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Proteomics
Prostate
Urine
Prostatic Diseases
Proteins
Proteome
Biomarkers
Prostatic Neoplasms
Body Fluids
Body fluids
Technology
Tissue
Protein Databases
Atlases
Biopsy
Immune system
Immune System
Western Blotting
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cita questo

Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine. / Alessandro, Riccardo; Fontana, Simona; Kislinger, Thomas; Semmes, O. John; Fontana, Simona; Medin, Jeffrey A.; Troyer, Dean A.; Drake, Richard R.; Nyalwidhe, Julius O.; Ignatchenko, Vladimir; Principe, Simona; Lance, Raymond S.; Kim, Yunee.

In: Journal of Proteome Research, Vol. 11, 2012, pag. 2386-2396.

Risultato della ricerca: Article

Alessandro, R, Fontana, S, Kislinger, T, Semmes, OJ, Fontana, S, Medin, JA, Troyer, DA, Drake, RR, Nyalwidhe, JO, Ignatchenko, V, Principe, S, Lance, RS & Kim, Y 2012, 'Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine', Journal of Proteome Research, vol. 11, pagg. 2386-2396.
Alessandro, Riccardo ; Fontana, Simona ; Kislinger, Thomas ; Semmes, O. John ; Fontana, Simona ; Medin, Jeffrey A. ; Troyer, Dean A. ; Drake, Richard R. ; Nyalwidhe, Julius O. ; Ignatchenko, Vladimir ; Principe, Simona ; Lance, Raymond S. ; Kim, Yunee. / Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine. In: Journal of Proteome Research. 2012 ; Vol. 11. pagg. 2386-2396.
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abstract = "Urinary expressed prostatic secretion or “EPS-urine” is proximal tissue fluid that is collected after a digital rectal exam (DRE). EPS-urine is a rich source of prostatederived proteins that can be used for biomarker discovery for prostate cancer (PCa) and other prostatic diseases. We previously conducted a comprehensive proteome analysis of direct expressed prostatic secretions (EPS). In the current study, we defined the proteome of EPS-urine employing Multidimensional Protein Identification Technology (MudPIT) and providing a comprehensive catalogue of this body fluid for future biomarker studies. We identified 1022 unique proteins in a heterogeneous cohort of 11 EPS-urines derived from biopsy negative noncancer diagnoses with some benign prostatic diseases (BPH) and lowgrade PCa, representative of secreted prostate and immune system-derived proteins in a urine background. We further applied MudPIT-based proteomics to generate and compare the differential proteome from a subset of pooled urines (pre-DRE) and EPS-urines (post- DRE) from noncancer and PCa patients. The direct proteomic comparison of these highly controlled patient sample pools enabled us to define a list of prostate-enriched proteins detectable in EPS-urine and distinguishable from a complex urine protein background. A combinatorial analysis of both proteomics data sets and systematic integration with publicly available proteomics data of related body fluids, human tissue transcriptomic data, and immunohistochemistry images from the Human Protein Atlas database allowed us to demarcate a robust panel of 49 prostate-derived proteins in EPS-urine. Finally, we validated the expression of seven of these proteins using Western blotting, supporting the likelihood that they originate from the prostate. The definition of these prostatic proteins in EPS-urine samples provides a reference for future investigations for prostatic-disease biomarker studies.",
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author = "Riccardo Alessandro and Simona Fontana and Thomas Kislinger and Semmes, {O. John} and Simona Fontana and Medin, {Jeffrey A.} and Troyer, {Dean A.} and Drake, {Richard R.} and Nyalwidhe, {Julius O.} and Vladimir Ignatchenko and Simona Principe and Lance, {Raymond S.} and Yunee Kim",
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T1 - Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine

AU - Alessandro, Riccardo

AU - Fontana, Simona

AU - Kislinger, Thomas

AU - Semmes, O. John

AU - Fontana, Simona

AU - Medin, Jeffrey A.

AU - Troyer, Dean A.

AU - Drake, Richard R.

AU - Nyalwidhe, Julius O.

AU - Ignatchenko, Vladimir

AU - Principe, Simona

AU - Lance, Raymond S.

AU - Kim, Yunee

PY - 2012

Y1 - 2012

N2 - Urinary expressed prostatic secretion or “EPS-urine” is proximal tissue fluid that is collected after a digital rectal exam (DRE). EPS-urine is a rich source of prostatederived proteins that can be used for biomarker discovery for prostate cancer (PCa) and other prostatic diseases. We previously conducted a comprehensive proteome analysis of direct expressed prostatic secretions (EPS). In the current study, we defined the proteome of EPS-urine employing Multidimensional Protein Identification Technology (MudPIT) and providing a comprehensive catalogue of this body fluid for future biomarker studies. We identified 1022 unique proteins in a heterogeneous cohort of 11 EPS-urines derived from biopsy negative noncancer diagnoses with some benign prostatic diseases (BPH) and lowgrade PCa, representative of secreted prostate and immune system-derived proteins in a urine background. We further applied MudPIT-based proteomics to generate and compare the differential proteome from a subset of pooled urines (pre-DRE) and EPS-urines (post- DRE) from noncancer and PCa patients. The direct proteomic comparison of these highly controlled patient sample pools enabled us to define a list of prostate-enriched proteins detectable in EPS-urine and distinguishable from a complex urine protein background. A combinatorial analysis of both proteomics data sets and systematic integration with publicly available proteomics data of related body fluids, human tissue transcriptomic data, and immunohistochemistry images from the Human Protein Atlas database allowed us to demarcate a robust panel of 49 prostate-derived proteins in EPS-urine. Finally, we validated the expression of seven of these proteins using Western blotting, supporting the likelihood that they originate from the prostate. The definition of these prostatic proteins in EPS-urine samples provides a reference for future investigations for prostatic-disease biomarker studies.

AB - Urinary expressed prostatic secretion or “EPS-urine” is proximal tissue fluid that is collected after a digital rectal exam (DRE). EPS-urine is a rich source of prostatederived proteins that can be used for biomarker discovery for prostate cancer (PCa) and other prostatic diseases. We previously conducted a comprehensive proteome analysis of direct expressed prostatic secretions (EPS). In the current study, we defined the proteome of EPS-urine employing Multidimensional Protein Identification Technology (MudPIT) and providing a comprehensive catalogue of this body fluid for future biomarker studies. We identified 1022 unique proteins in a heterogeneous cohort of 11 EPS-urines derived from biopsy negative noncancer diagnoses with some benign prostatic diseases (BPH) and lowgrade PCa, representative of secreted prostate and immune system-derived proteins in a urine background. We further applied MudPIT-based proteomics to generate and compare the differential proteome from a subset of pooled urines (pre-DRE) and EPS-urines (post- DRE) from noncancer and PCa patients. The direct proteomic comparison of these highly controlled patient sample pools enabled us to define a list of prostate-enriched proteins detectable in EPS-urine and distinguishable from a complex urine protein background. A combinatorial analysis of both proteomics data sets and systematic integration with publicly available proteomics data of related body fluids, human tissue transcriptomic data, and immunohistochemistry images from the Human Protein Atlas database allowed us to demarcate a robust panel of 49 prostate-derived proteins in EPS-urine. Finally, we validated the expression of seven of these proteins using Western blotting, supporting the likelihood that they originate from the prostate. The definition of these prostatic proteins in EPS-urine samples provides a reference for future investigations for prostatic-disease biomarker studies.

KW - Proteomics, prostate cancer, expressed prostatic secretions, urine

UR - http://hdl.handle.net/10447/75385

M3 - Article

VL - 11

SP - 2386

EP - 2396

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

ER -