Identification of pathways involved in aneuploidy onset and its tolerance using a DNA microarray approach

Risultato della ricerca: Paper

Abstract

Genomic instability is a hallmark of the majority of human tumors explaining the heterogeneity shown by tumor cells. This phenomenon is often associated with chromosomal instability (CIN) and aneuploidy, a condition in which tumor cells lose or gain chromosomes. Previously, we showed that posttranscriptional silencing by RNAi of pRb1, DNMT12 and MAD2 is associated with aneuploidy in cultured human cells reinforcing the idea that there are several roads leading to aneuploidy. In the attempt to understand if a common molecular signature exists underlying aneuploidy and its tolerance in tumor cells, we induced aneuploidy in human fibroblasts (IMR90) by depleting Rb, MAD2 and DNMT1 genes and analyzed their transcriptome by Microarray (using a p-value of 0.05 and a fold change greater than 2). 1722 differentially expressed genes in the three sample analyzed against control were identified, of which 282 differentially expressed simultaneously in at least two out of three samples analyzed. These 282 genes were analyzed using freeware bioinformatics software (DAVID, GOrilla) that showed the presence of genes involved in many functional group inherent to the inflammatory response, cell proliferation and apoptosis. A detailed analysis of these results (using other system biology tools like Pathway Studio 9, Reactome, STRING) will be shown to get clues about the pathways involved in the generation and tolerance of aneuploidy.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2013

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Aneuploidy
Oligonucleotide Array Sequence Analysis
Genes
Neoplasms
Gorilla gorilla
Chromosomal Instability
Systems Biology
Genomic Instability
RNA Interference
Computational Biology
Transcriptome
Cultured Cells
Software
Fibroblasts
Chromosomes
Cell Proliferation
Apoptosis

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title = "Identification of pathways involved in aneuploidy onset and its tolerance using a DNA microarray approach",
abstract = "Genomic instability is a hallmark of the majority of human tumors explaining the heterogeneity shown by tumor cells. This phenomenon is often associated with chromosomal instability (CIN) and aneuploidy, a condition in which tumor cells lose or gain chromosomes. Previously, we showed that posttranscriptional silencing by RNAi of pRb1, DNMT12 and MAD2 is associated with aneuploidy in cultured human cells reinforcing the idea that there are several roads leading to aneuploidy. In the attempt to understand if a common molecular signature exists underlying aneuploidy and its tolerance in tumor cells, we induced aneuploidy in human fibroblasts (IMR90) by depleting Rb, MAD2 and DNMT1 genes and analyzed their transcriptome by Microarray (using a p-value of 0.05 and a fold change greater than 2). 1722 differentially expressed genes in the three sample analyzed against control were identified, of which 282 differentially expressed simultaneously in at least two out of three samples analyzed. These 282 genes were analyzed using freeware bioinformatics software (DAVID, GOrilla) that showed the presence of genes involved in many functional group inherent to the inflammatory response, cell proliferation and apoptosis. A detailed analysis of these results (using other system biology tools like Pathway Studio 9, Reactome, STRING) will be shown to get clues about the pathways involved in the generation and tolerance of aneuploidy.",
author = "{Di Leonardo}, Aldo and Laura Lentini and Giuseppe Costa and Sergio Spatafora",
year = "2013",
language = "English",

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TY - CONF

T1 - Identification of pathways involved in aneuploidy onset and its tolerance using a DNA microarray approach

AU - Di Leonardo, Aldo

AU - Lentini, Laura

AU - Costa, Giuseppe

AU - Spatafora, Sergio

PY - 2013

Y1 - 2013

N2 - Genomic instability is a hallmark of the majority of human tumors explaining the heterogeneity shown by tumor cells. This phenomenon is often associated with chromosomal instability (CIN) and aneuploidy, a condition in which tumor cells lose or gain chromosomes. Previously, we showed that posttranscriptional silencing by RNAi of pRb1, DNMT12 and MAD2 is associated with aneuploidy in cultured human cells reinforcing the idea that there are several roads leading to aneuploidy. In the attempt to understand if a common molecular signature exists underlying aneuploidy and its tolerance in tumor cells, we induced aneuploidy in human fibroblasts (IMR90) by depleting Rb, MAD2 and DNMT1 genes and analyzed their transcriptome by Microarray (using a p-value of 0.05 and a fold change greater than 2). 1722 differentially expressed genes in the three sample analyzed against control were identified, of which 282 differentially expressed simultaneously in at least two out of three samples analyzed. These 282 genes were analyzed using freeware bioinformatics software (DAVID, GOrilla) that showed the presence of genes involved in many functional group inherent to the inflammatory response, cell proliferation and apoptosis. A detailed analysis of these results (using other system biology tools like Pathway Studio 9, Reactome, STRING) will be shown to get clues about the pathways involved in the generation and tolerance of aneuploidy.

AB - Genomic instability is a hallmark of the majority of human tumors explaining the heterogeneity shown by tumor cells. This phenomenon is often associated with chromosomal instability (CIN) and aneuploidy, a condition in which tumor cells lose or gain chromosomes. Previously, we showed that posttranscriptional silencing by RNAi of pRb1, DNMT12 and MAD2 is associated with aneuploidy in cultured human cells reinforcing the idea that there are several roads leading to aneuploidy. In the attempt to understand if a common molecular signature exists underlying aneuploidy and its tolerance in tumor cells, we induced aneuploidy in human fibroblasts (IMR90) by depleting Rb, MAD2 and DNMT1 genes and analyzed their transcriptome by Microarray (using a p-value of 0.05 and a fold change greater than 2). 1722 differentially expressed genes in the three sample analyzed against control were identified, of which 282 differentially expressed simultaneously in at least two out of three samples analyzed. These 282 genes were analyzed using freeware bioinformatics software (DAVID, GOrilla) that showed the presence of genes involved in many functional group inherent to the inflammatory response, cell proliferation and apoptosis. A detailed analysis of these results (using other system biology tools like Pathway Studio 9, Reactome, STRING) will be shown to get clues about the pathways involved in the generation and tolerance of aneuploidy.

UR - http://hdl.handle.net/10447/104192

UR - http://agi.unipr.it/eventi/att/5bdd.allegato.09478.pdf

M3 - Paper

ER -