Identification of circumferential regional heterogeneity of ascending thoracic aneurysmal aorta by biaxial mechanical testing

Marzio Di Giuseppe, Salvatore Pasta, Gioacchino Alotta, Valeria Vetri, Massimiliano Zingales, Valentina Agnese, Michele Pilato, Diego Bellavia, Giuseppe M. Raffa, Salvatore Pasta, Valentina Agnese, Giorgia Maria Giovanna Raffa

Risultato della ricerca: Article

1 Citazione (Scopus)

Abstract

Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) can present an asymmetrical aortic dilatation compared with patients with tricuspid aortic valve (TAV). This pattern of aneurysm dilatation led us to hypothesize that biomechanical differences likely induced by regional heterogeneity of material properties can underlie the observed asymmetric enlargement discrepancies between BAV ATAA and TAV ATAA. This study aimed to characterize the mechanical properties and associated aortic tissue stiffness changes along the circumferential direction of aortic rings collected from surgically-repaired patients with ATAA. Biaxial material testing was performed on tissue specimens extrapolated from all aortic quadrants (i.e. anterior, posterior, major and minor curvature of the aorta), and then the tissue stiffness was quantified at both physiological and supra-physiological stress levels (i.e. 142 kPa and 242 kPa, respectively). Tissue stiffness revealed that the major curvature of BAV ATAA is statistically less stiff than the anterior quadrant (276.6 +/- 137.1 kPa for BAV ATAA and 830.1 +/- 557.1 kPa for BAV ATAA, p = .024, at 142 kPa) and to that of major curvature of TAV ATAA (276.6 +/- 137.0 kPa for BAV ATAA and 733.2 +/- 391.1 kPa for TAV ATAA, p = .001, at 142 kPa), suggesting local weakening of bicuspid aortic wall. Multiphoton imaging revealed local changes on elastic fiber networks. The recovered material parameters for the Fung-type constitutive model are crucial for reliable stress predictions while the information on regional tissue stiffness changes are fundamental to develop risk stratification strategies not based on aortic size.
Lingua originaleEnglish
pagine (da-a)205-215
Numero di pagine11
RivistaJournal of Molecular and Cellular Cardiology
Volume130
Stato di pubblicazionePublished - 2019

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Thoracic Aortic Aneurysm
Thoracic Aorta
Tricuspid Valve
Aortic Valve
Dilatation
Materials Testing
Vascular Stiffness
Physiological Stress
Elastic Tissue
Bicuspid
Aneurysm
Aorta
Bicuspid Aortic Valve

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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Identification of circumferential regional heterogeneity of ascending thoracic aneurysmal aorta by biaxial mechanical testing. / Di Giuseppe, Marzio; Pasta, Salvatore; Alotta, Gioacchino; Vetri, Valeria; Zingales, Massimiliano; Agnese, Valentina; Pilato, Michele; Bellavia, Diego; Raffa, Giuseppe M.; Pasta, Salvatore; Agnese, Valentina; Raffa, Giorgia Maria Giovanna.

In: Journal of Molecular and Cellular Cardiology, Vol. 130, 2019, pag. 205-215.

Risultato della ricerca: Article

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title = "Identification of circumferential regional heterogeneity of ascending thoracic aneurysmal aorta by biaxial mechanical testing",
abstract = "Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) can present an asymmetrical aortic dilatation compared with patients with tricuspid aortic valve (TAV). This pattern of aneurysm dilatation led us to hypothesize that biomechanical differences likely induced by regional heterogeneity of material properties can underlie the observed asymmetric enlargement discrepancies between BAV ATAA and TAV ATAA. This study aimed to characterize the mechanical properties and associated aortic tissue stiffness changes along the circumferential direction of aortic rings collected from surgically-repaired patients with ATAA. Biaxial material testing was performed on tissue specimens extrapolated from all aortic quadrants (i.e. anterior, posterior, major and minor curvature of the aorta), and then the tissue stiffness was quantified at both physiological and supra-physiological stress levels (i.e. 142 kPa and 242 kPa, respectively). Tissue stiffness revealed that the major curvature of BAV ATAA is statistically less stiff than the anterior quadrant (276.6 +/- 137.1 kPa for BAV ATAA and 830.1 +/- 557.1 kPa for BAV ATAA, p = .024, at 142 kPa) and to that of major curvature of TAV ATAA (276.6 +/- 137.0 kPa for BAV ATAA and 733.2 +/- 391.1 kPa for TAV ATAA, p = .001, at 142 kPa), suggesting local weakening of bicuspid aortic wall. Multiphoton imaging revealed local changes on elastic fiber networks. The recovered material parameters for the Fung-type constitutive model are crucial for reliable stress predictions while the information on regional tissue stiffness changes are fundamental to develop risk stratification strategies not based on aortic size.",
author = "{Di Giuseppe}, Marzio and Salvatore Pasta and Gioacchino Alotta and Valeria Vetri and Massimiliano Zingales and Valentina Agnese and Michele Pilato and Diego Bellavia and Raffa, {Giuseppe M.} and Salvatore Pasta and Valentina Agnese and Raffa, {Giorgia Maria Giovanna}",
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T1 - Identification of circumferential regional heterogeneity of ascending thoracic aneurysmal aorta by biaxial mechanical testing

AU - Di Giuseppe, Marzio

AU - Pasta, Salvatore

AU - Alotta, Gioacchino

AU - Vetri, Valeria

AU - Zingales, Massimiliano

AU - Agnese, Valentina

AU - Pilato, Michele

AU - Bellavia, Diego

AU - Raffa, Giuseppe M.

AU - Pasta, Salvatore

AU - Agnese, Valentina

AU - Raffa, Giorgia Maria Giovanna

PY - 2019

Y1 - 2019

N2 - Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) can present an asymmetrical aortic dilatation compared with patients with tricuspid aortic valve (TAV). This pattern of aneurysm dilatation led us to hypothesize that biomechanical differences likely induced by regional heterogeneity of material properties can underlie the observed asymmetric enlargement discrepancies between BAV ATAA and TAV ATAA. This study aimed to characterize the mechanical properties and associated aortic tissue stiffness changes along the circumferential direction of aortic rings collected from surgically-repaired patients with ATAA. Biaxial material testing was performed on tissue specimens extrapolated from all aortic quadrants (i.e. anterior, posterior, major and minor curvature of the aorta), and then the tissue stiffness was quantified at both physiological and supra-physiological stress levels (i.e. 142 kPa and 242 kPa, respectively). Tissue stiffness revealed that the major curvature of BAV ATAA is statistically less stiff than the anterior quadrant (276.6 +/- 137.1 kPa for BAV ATAA and 830.1 +/- 557.1 kPa for BAV ATAA, p = .024, at 142 kPa) and to that of major curvature of TAV ATAA (276.6 +/- 137.0 kPa for BAV ATAA and 733.2 +/- 391.1 kPa for TAV ATAA, p = .001, at 142 kPa), suggesting local weakening of bicuspid aortic wall. Multiphoton imaging revealed local changes on elastic fiber networks. The recovered material parameters for the Fung-type constitutive model are crucial for reliable stress predictions while the information on regional tissue stiffness changes are fundamental to develop risk stratification strategies not based on aortic size.

AB - Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) can present an asymmetrical aortic dilatation compared with patients with tricuspid aortic valve (TAV). This pattern of aneurysm dilatation led us to hypothesize that biomechanical differences likely induced by regional heterogeneity of material properties can underlie the observed asymmetric enlargement discrepancies between BAV ATAA and TAV ATAA. This study aimed to characterize the mechanical properties and associated aortic tissue stiffness changes along the circumferential direction of aortic rings collected from surgically-repaired patients with ATAA. Biaxial material testing was performed on tissue specimens extrapolated from all aortic quadrants (i.e. anterior, posterior, major and minor curvature of the aorta), and then the tissue stiffness was quantified at both physiological and supra-physiological stress levels (i.e. 142 kPa and 242 kPa, respectively). Tissue stiffness revealed that the major curvature of BAV ATAA is statistically less stiff than the anterior quadrant (276.6 +/- 137.1 kPa for BAV ATAA and 830.1 +/- 557.1 kPa for BAV ATAA, p = .024, at 142 kPa) and to that of major curvature of TAV ATAA (276.6 +/- 137.0 kPa for BAV ATAA and 733.2 +/- 391.1 kPa for TAV ATAA, p = .001, at 142 kPa), suggesting local weakening of bicuspid aortic wall. Multiphoton imaging revealed local changes on elastic fiber networks. The recovered material parameters for the Fung-type constitutive model are crucial for reliable stress predictions while the information on regional tissue stiffness changes are fundamental to develop risk stratification strategies not based on aortic size.

UR - http://hdl.handle.net/10447/376238

UR - http://www.elsevier.com/locate/yjmcc

M3 - Article

VL - 130

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EP - 215

JO - Journal of Molecular and Cellular Cardiology

JF - Journal of Molecular and Cellular Cardiology

SN - 0022-2828

ER -