IDENTIFICATION OF BACTERIAL TAXA IN ARCHAEOLOGICAL WATERLOGGED WOOD

Franco Palla, Enza Di Carlo, Giovanna Benedetta Barresi

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5 Citazioni (Scopus)

Abstract

The microscopic and molecular techniques described in this study are aimed at understanding the degradation processes of the anatomical structure of submerged archaeological wood, correlating it to the degradation induced by bacteria. The SEM micrographs showed alterations of the wooden structure due to bacterial colonization, as well as the presence of pyrite framboids. The difficulty of extracting bacterial DNA from wooden fragments belonging to submerged finds is well-known, due to the presence of many inhibitors; this study described some extraction and in vitro amplification protocols for wooden submerged finds. The results of the molecular investigations, based on the analysis of specific sequences of microbial genomic DNA enable us to detect the presence of cellulolytic and ligninolytic bacteria, in addition to iron-oxidizing or sulfate-reducing bacteria, otherwise undetectable by traditional in vitro culture methods.
Lingua originaleEnglish
pagine (da-a)247-254
Numero di pagine8
RivistaConservation Science in Cultural Heritage
Volume14
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

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