Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex

Silvio Fici, Francesco Carimi, Giuseppe Garfì, Mirko Siragusa, Ignazio Fontana, Alessandro Silvestre Gristina

Risultato della ricerca: Article

8 Citazioni (Scopus)

Abstract

Inter–Simple Sequence Repeat (ISSR) molecular markers and morphological analysis were used in order to characterize wild populations and cultivated forms of orphan crop species Capparis spinosa L. in a Mediterranean island complex. Nineteen wild populations belonging to two different subspecies, C. spinosa subsp. spinosa and subsp. rupestris, were sampled in different environments in Sicily and the surrounding islets Lampedusa, Pantelleria and Salina. Different biotypes cultivated in Pantelleria and Salina were analyzed. Six ISSR primers were selected for genetic characterization, and all clear and reproducible bands were scored and analyzed. Among the 47 ISSR bands obtained, 97.5% were polymorphic. Results of AMOVA and STRUCTURE analysis suggested a clear genetic distinctness between subspecies at the regional level and suggested the existence of two taxonomic groups among wild populations, with different ecological preferences and distinctive morphological characters. Cultivated forms showed genetic affinity to subsp. rupestris. ISSR analysis not only provided specific molecular markers to discriminate the taxa, but also proved useful in supporting the hypothesis of a hybrid origin of the intermediate phenotypes found in overlapping distribution areas. The identified molecular markers provided a basic tool for the DNA fingerprinting of wild and commercial capers in the Mediterranean region and nearby territory.
Lingua originaleEnglish
pagine (da-a)-
Numero di pagine0
RivistaFlora
Volume209
Stato di pubblicazionePublished - 2014

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Capparis spinosa
Mediterranean Islands
wild population
hybridization
microsatellite repeats
subspecies
genetic markers
orphan
DNA fingerprinting
biotype
phenotype
Sicily
biotypes
Mediterranean region
crop
analysis
marker
crops
salina

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Ecology

Cita questo

Fici, S., Carimi, F., Garfì, G., Siragusa, M., Fontana, I., & Gristina, A. S. (2014). Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex. Flora, 209, -.

Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex. / Fici, Silvio; Carimi, Francesco; Garfì, Giuseppe; Siragusa, Mirko; Fontana, Ignazio; Gristina, Alessandro Silvestre.

In: Flora, Vol. 209, 2014, pag. -.

Risultato della ricerca: Article

Fici, S, Carimi, F, Garfì, G, Siragusa, M, Fontana, I & Gristina, AS 2014, 'Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex', Flora, vol. 209, pagg. -.
Fici, Silvio ; Carimi, Francesco ; Garfì, Giuseppe ; Siragusa, Mirko ; Fontana, Ignazio ; Gristina, Alessandro Silvestre. / Hybridization in Capparis spinosa L.: molecular and morphological evidence from a Mediterranean island complex. In: Flora. 2014 ; Vol. 209. pagg. -.
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abstract = "Inter–Simple Sequence Repeat (ISSR) molecular markers and morphological analysis were used in order to characterize wild populations and cultivated forms of orphan crop species Capparis spinosa L. in a Mediterranean island complex. Nineteen wild populations belonging to two different subspecies, C. spinosa subsp. spinosa and subsp. rupestris, were sampled in different environments in Sicily and the surrounding islets Lampedusa, Pantelleria and Salina. Different biotypes cultivated in Pantelleria and Salina were analyzed. Six ISSR primers were selected for genetic characterization, and all clear and reproducible bands were scored and analyzed. Among the 47 ISSR bands obtained, 97.5{\%} were polymorphic. Results of AMOVA and STRUCTURE analysis suggested a clear genetic distinctness between subspecies at the regional level and suggested the existence of two taxonomic groups among wild populations, with different ecological preferences and distinctive morphological characters. Cultivated forms showed genetic affinity to subsp. rupestris. ISSR analysis not only provided specific molecular markers to discriminate the taxa, but also proved useful in supporting the hypothesis of a hybrid origin of the intermediate phenotypes found in overlapping distribution areas. The identified molecular markers provided a basic tool for the DNA fingerprinting of wild and commercial capers in the Mediterranean region and nearby territory.",
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AU - Fici, Silvio

AU - Carimi, Francesco

AU - Garfì, Giuseppe

AU - Siragusa, Mirko

AU - Fontana, Ignazio

AU - Gristina, Alessandro Silvestre

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N2 - Inter–Simple Sequence Repeat (ISSR) molecular markers and morphological analysis were used in order to characterize wild populations and cultivated forms of orphan crop species Capparis spinosa L. in a Mediterranean island complex. Nineteen wild populations belonging to two different subspecies, C. spinosa subsp. spinosa and subsp. rupestris, were sampled in different environments in Sicily and the surrounding islets Lampedusa, Pantelleria and Salina. Different biotypes cultivated in Pantelleria and Salina were analyzed. Six ISSR primers were selected for genetic characterization, and all clear and reproducible bands were scored and analyzed. Among the 47 ISSR bands obtained, 97.5% were polymorphic. Results of AMOVA and STRUCTURE analysis suggested a clear genetic distinctness between subspecies at the regional level and suggested the existence of two taxonomic groups among wild populations, with different ecological preferences and distinctive morphological characters. Cultivated forms showed genetic affinity to subsp. rupestris. ISSR analysis not only provided specific molecular markers to discriminate the taxa, but also proved useful in supporting the hypothesis of a hybrid origin of the intermediate phenotypes found in overlapping distribution areas. The identified molecular markers provided a basic tool for the DNA fingerprinting of wild and commercial capers in the Mediterranean region and nearby territory.

AB - Inter–Simple Sequence Repeat (ISSR) molecular markers and morphological analysis were used in order to characterize wild populations and cultivated forms of orphan crop species Capparis spinosa L. in a Mediterranean island complex. Nineteen wild populations belonging to two different subspecies, C. spinosa subsp. spinosa and subsp. rupestris, were sampled in different environments in Sicily and the surrounding islets Lampedusa, Pantelleria and Salina. Different biotypes cultivated in Pantelleria and Salina were analyzed. Six ISSR primers were selected for genetic characterization, and all clear and reproducible bands were scored and analyzed. Among the 47 ISSR bands obtained, 97.5% were polymorphic. Results of AMOVA and STRUCTURE analysis suggested a clear genetic distinctness between subspecies at the regional level and suggested the existence of two taxonomic groups among wild populations, with different ecological preferences and distinctive morphological characters. Cultivated forms showed genetic affinity to subsp. rupestris. ISSR analysis not only provided specific molecular markers to discriminate the taxa, but also proved useful in supporting the hypothesis of a hybrid origin of the intermediate phenotypes found in overlapping distribution areas. The identified molecular markers provided a basic tool for the DNA fingerprinting of wild and commercial capers in the Mediterranean region and nearby territory.

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