Hyaluronic acid and α-elastin based hydrogel for three dimensional culture of vascular endothelial cells

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1 Citazione (Scopus)

Abstract

The aim of this work was to demonstrate that employing a copolymer of hyaluronic acid and α-elastin (HA-EDA-g-α-elastin) is possible to produce a scaffold able to support the adhesion and growth of human vascular endothelial cells (HUVEC) thanks to its ability to incorporate and control the diffusion of vascular endothelial growth factor (VEGF). HA-EDA-g-α-elastin was crosslinked with low molecular weight hyaluronic acid (HALMW) to obtain a hydrogel that gives rise to the formation of three dimensional sponge after the freeze drying process. The physicochemical features the obtained material along with its ability to act as a support for the three dimensional culture of endothelial cells were here investigated. The obtained hydrogel is able to seize the VEGF and control its release in the first days of incubation. The presence of the growth factor stimulates the proliferation of HUVEC and induces the formation of tubular structures.
Lingua originaleEnglish
pagine (da-a)28-33
Numero di pagine6
RivistaJournal of Drug Delivery Science and Technology
Volume46
Stato di pubblicazionePublished - 2018

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Elastin
Hydrogel
Hyaluronic Acid
Endothelial Cells
Vascular Endothelial Growth Factor A
Freeze Drying
Porifera
Intercellular Signaling Peptides and Proteins
Molecular Weight
Growth

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cita questo

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title = "Hyaluronic acid and α-elastin based hydrogel for three dimensional culture of vascular endothelial cells",
abstract = "The aim of this work was to demonstrate that employing a copolymer of hyaluronic acid and α-elastin (HA-EDA-g-α-elastin) is possible to produce a scaffold able to support the adhesion and growth of human vascular endothelial cells (HUVEC) thanks to its ability to incorporate and control the diffusion of vascular endothelial growth factor (VEGF). HA-EDA-g-α-elastin was crosslinked with low molecular weight hyaluronic acid (HALMW) to obtain a hydrogel that gives rise to the formation of three dimensional sponge after the freeze drying process. The physicochemical features the obtained material along with its ability to act as a support for the three dimensional culture of endothelial cells were here investigated. The obtained hydrogel is able to seize the VEGF and control its release in the first days of incubation. The presence of the growth factor stimulates the proliferation of HUVEC and induces the formation of tubular structures.",
keywords = "3003, Elastin, Hyaluronic acid, Hydrogel, Vascular endothelial growth factor, Wound healing",
author = "Gaetano Giammona and Giovanna Pitarresi and Mario Allegra and Palumbo, {Fabio Salvatore} and Calogero Fiorica and Roberto Puleio and Gaetano Giammona",
year = "2018",
language = "English",
volume = "46",
pages = "28--33",
journal = "Journal of Drug Delivery Science and Technology",
issn = "1773-2247",
publisher = "Editions de Sante",

}

TY - JOUR

T1 - Hyaluronic acid and α-elastin based hydrogel for three dimensional culture of vascular endothelial cells

AU - Giammona, Gaetano

AU - Pitarresi, Giovanna

AU - Allegra, Mario

AU - Palumbo, Fabio Salvatore

AU - Fiorica, Calogero

AU - Puleio, Roberto

AU - Giammona, Gaetano

PY - 2018

Y1 - 2018

N2 - The aim of this work was to demonstrate that employing a copolymer of hyaluronic acid and α-elastin (HA-EDA-g-α-elastin) is possible to produce a scaffold able to support the adhesion and growth of human vascular endothelial cells (HUVEC) thanks to its ability to incorporate and control the diffusion of vascular endothelial growth factor (VEGF). HA-EDA-g-α-elastin was crosslinked with low molecular weight hyaluronic acid (HALMW) to obtain a hydrogel that gives rise to the formation of three dimensional sponge after the freeze drying process. The physicochemical features the obtained material along with its ability to act as a support for the three dimensional culture of endothelial cells were here investigated. The obtained hydrogel is able to seize the VEGF and control its release in the first days of incubation. The presence of the growth factor stimulates the proliferation of HUVEC and induces the formation of tubular structures.

AB - The aim of this work was to demonstrate that employing a copolymer of hyaluronic acid and α-elastin (HA-EDA-g-α-elastin) is possible to produce a scaffold able to support the adhesion and growth of human vascular endothelial cells (HUVEC) thanks to its ability to incorporate and control the diffusion of vascular endothelial growth factor (VEGF). HA-EDA-g-α-elastin was crosslinked with low molecular weight hyaluronic acid (HALMW) to obtain a hydrogel that gives rise to the formation of three dimensional sponge after the freeze drying process. The physicochemical features the obtained material along with its ability to act as a support for the three dimensional culture of endothelial cells were here investigated. The obtained hydrogel is able to seize the VEGF and control its release in the first days of incubation. The presence of the growth factor stimulates the proliferation of HUVEC and induces the formation of tubular structures.

KW - 3003

KW - Elastin

KW - Hyaluronic acid

KW - Hydrogel

KW - Vascular endothelial growth factor

KW - Wound healing

UR - http://hdl.handle.net/10447/296864

UR - http://www.editionsdesante.fr/category.php?id_category=48

M3 - Article

VL - 46

SP - 28

EP - 33

JO - Journal of Drug Delivery Science and Technology

JF - Journal of Drug Delivery Science and Technology

SN - 1773-2247

ER -