TY - JOUR
T1 - Hyaluronan alkyl derivatives-based electrospun membranes for potential guided bone regeneration: Fabrication, characterization and in vitro osteoinductive properties
AU - Federico, Salvatore
AU - Fiorica, Calogero
AU - Palumbo, Fabio Salvatore
AU - Pitarresi, Giovanna
AU - Giammona, Gaetano
AU - Yang, Fang
AU - Yang, Fang
AU - Giammona, Gaetano
PY - 2021
Y1 - 2021
N2 - The aim of the work was to determine the effects of the chemical functionalization of hyaluronic acid (HA) with pendant aliphatic tails at different lengths and free amino groups in terms of chemical reactivity, degradation rate, drug-eluting features, and surface properties when processed as electrospun membranes (EM) evaluating the osteoinductive potential for a possible application as guided bone regeneration (GBR). To this end, a series of HA derivatives with different aliphatic tails (DD-Cx mol% ≈ 12.0 mol%) and decreasing derivatization of free amino groups (DDEDA mol% from 70.0 to 30.0 mol%) were first synthesized, namely Hn. Then dexamethasone-loaded Hn EM, i.e. HnX were prepared from aqueous polymeric solutions with polyvinyl alcohol (PVA), as a non-ionogenic linear flexible polymeric carrier, and the multifunctional 2-hydroxypropyl- cyclodextrin (HPCD) which acted as a rheological modifier, a stabilizer of Taylor's cone, and a solubilizing agent. A comprehensive characterization of the membranes was carried out through ATR-IR, XRD, and WCA measurements. According to the in vitro hydrolytic and enzymatic degradation and drug release in different aqueous media for two months, the insertion of alkyl pendant grafts and the crosslinking process provided tuneable additional resistance to the whole membrane suitably for the final application of the membranes. Cell culture showed the cytocompatibility and cell proliferation until 7 days. Osteogenic differentiation and mineralization of pre-osteoblastic MC3T3 cells occurred for most of membranes after 35 days as valued by measuring ALP activity (50 nmol 4-np/h/nf DNA) and the deposition of calcium (120−140 μg ml−1).
AB - The aim of the work was to determine the effects of the chemical functionalization of hyaluronic acid (HA) with pendant aliphatic tails at different lengths and free amino groups in terms of chemical reactivity, degradation rate, drug-eluting features, and surface properties when processed as electrospun membranes (EM) evaluating the osteoinductive potential for a possible application as guided bone regeneration (GBR). To this end, a series of HA derivatives with different aliphatic tails (DD-Cx mol% ≈ 12.0 mol%) and decreasing derivatization of free amino groups (DDEDA mol% from 70.0 to 30.0 mol%) were first synthesized, namely Hn. Then dexamethasone-loaded Hn EM, i.e. HnX were prepared from aqueous polymeric solutions with polyvinyl alcohol (PVA), as a non-ionogenic linear flexible polymeric carrier, and the multifunctional 2-hydroxypropyl- cyclodextrin (HPCD) which acted as a rheological modifier, a stabilizer of Taylor's cone, and a solubilizing agent. A comprehensive characterization of the membranes was carried out through ATR-IR, XRD, and WCA measurements. According to the in vitro hydrolytic and enzymatic degradation and drug release in different aqueous media for two months, the insertion of alkyl pendant grafts and the crosslinking process provided tuneable additional resistance to the whole membrane suitably for the final application of the membranes. Cell culture showed the cytocompatibility and cell proliferation until 7 days. Osteogenic differentiation and mineralization of pre-osteoblastic MC3T3 cells occurred for most of membranes after 35 days as valued by measuring ALP activity (50 nmol 4-np/h/nf DNA) and the deposition of calcium (120−140 μg ml−1).
KW - Dexamethasone
KW - Electrospun nanofibers
KW - GBR membranes
KW - Hyaluronic acid alkylated
KW - Dexamethasone
KW - Electrospun nanofibers
KW - GBR membranes
KW - Hyaluronic acid alkylated
UR - http://hdl.handle.net/10447/443445
M3 - Article
VL - 197
SP - 1
EP - 10
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -