Human umbilical cord expresses several vasoactivepeptides involved in the local regulation of vasculartone: protein and gene expression of Orphanin,Oxytocin, ANP, eNOS and iNOS

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    Abstract

    Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein andsurrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellularmatrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow.The aim of this study was to investigate in human umbilical cord the expression of five peptides that could beinvolved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelialNitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). The expression of these moleculesin full-term human umbilical cord was investigated through immunohistochemistry and RT-PCR. Immunoreactivityfor Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin,ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOSonly by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOSmRNAs. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in the humanumbilical cord. mRNA expression for Oxytocin, ANP and eNOS suggest that these molecules are synthesized byumbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanismlocally regulating vascular tone.
    Lingua originaleEnglish
    pagine (da-a)211-218
    Numero di pagine8
    RivistaFolia Histochemica et Cytobiologica
    Volume49
    Stato di pubblicazionePublished - 2011

    All Science Journal Classification (ASJC) codes

    • Pathology and Forensic Medicine
    • Histology

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