Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS

Buscemi, M; Provenzano, S; Gerbino, A

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    Abstract

    Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.
    Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.
    Lingua originaleEnglish
    pagine (da-a)-
    Numero di pagine8
    RivistaFolia Histochemica et Cytobiologica
    Volume49(2)
    Stato di pubblicazionePublished - 2011

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    Nitric Oxide Synthase Type III
    Umbilical Cord
    Atrial Natriuretic Factor
    Nitric Oxide Synthase Type II
    Oxytocin
    Wharton Jelly
    Blood Vessels
    Gene Expression
    Umbilicus
    Proteins
    Endothelium
    Endothelial Cells
    Polymerase Chain Reaction
    Messenger RNA
    Mesenchymal Stromal Cells
    Extracellular Matrix
    Veins
    Epithelium
    Arteries
    Immunohistochemistry

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    title = "Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS",
    abstract = "Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.",
    keywords = "Human umbilical cord, Orphanin, Oxytocin, ANP, eNOS, iNOS",
    author = "{Buscemi, M; Provenzano, S; Gerbino, A} and Annamaria Mauro",
    year = "2011",
    language = "English",
    volume = "49(2)",
    pages = "--",
    journal = "Folia Histochemica et Cytobiologica",
    issn = "0239-8508",
    publisher = "Polish Histochemical and Cytochemical Society",

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    TY - JOUR

    T1 - Human umbilical cord expresses several vasoactive peptides involved in the local regulation of vascular tone: protein and gene expression of Orphanin, Oxytocin, ANP, eNOS and iNOS

    AU - Buscemi, M; Provenzano, S; Gerbino, A

    AU - Mauro, Annamaria

    PY - 2011

    Y1 - 2011

    N2 - Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.

    AB - Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.Introduction. Full-term human umbilical cord contains three blood vessels: two arteries coiled around a vein and surrounded by Wharton’s jelly, a mucous tissue with few mesenchymal stromal cells and abundant extracellular matrix. Umbilical vessels lack innervations, thus endothelial cells must play a role in the control of blood flow. Objectives. The aim of this work was to investigate in human umbilical cord the expression of five peptides that could be involved in the regulation of vascular tone: Orphanin FQ, Oxytocin, Atrial Natriuretic Peptide (ANP), endothelial Nitric Oxide Synthase (eNOS) and inducible Nitric Oxide Synthase (iNOS). Methods. The expression of these molecules in full-term human umbilical cord has been investigated through immunohistochemistry and RT-PCR. Results. Immunoreactivity for Orphanin FQ was detected in Wharton’s jelly, vessel musculature and endothelium; Oxytocin, ANP and eNOS were expressed by the umbilical epithelium, Wharton’s jelly and endothelium, whereas iNOS only by endothelial cells. RT-PCR analysis showed transcriptional expression of Oxytocin, ANP and eNOS mRNAs. Conclusions. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in human umbilical cord, moreover mRNA expression for Oxytocin, ANP and eNOS suggests that these last molecules are synthesized by umbilical cord cells themselves. The expression of these vasoactive molecules could be part of a general mechanism aimed to locally regulate vascular tone.

    KW - Human umbilical cord, Orphanin, Oxytocin, ANP, eNOS, iNOS

    UR - http://hdl.handle.net/10447/53053

    M3 - Article

    VL - 49(2)

    SP - -

    JO - Folia Histochemica et Cytobiologica

    JF - Folia Histochemica et Cytobiologica

    SN - 0239-8508

    ER -