Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials

Sabrina David, Fabio Bucchieri, Francesco Cappello, Celeste Caruso Bavisotto, Claudia Campanella, Claudia Campanella, Beatrice Bocca, Francesco Petrucci, Alessandro Alimonti, Davide Mizzoni, Rossella Di Raimo, Mario Falchi, Daniela F. Angelini, Celeste Caruso Bavisotto, Stefano Caimi, Fabio Bucchieri, Mariantonia Logozzi, Stefano Fais, Luca Battistini, Francesco CappelloSabrina David

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.
Lingua originaleEnglish
pagine (da-a)23-36
Numero di pagine14
RivistaDefault journal
Volume137
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Pharmaceutical Science

Cita questo

Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials. / David, Sabrina; Bucchieri, Fabio; Cappello, Francesco; Caruso Bavisotto, Celeste; Campanella, Claudia; Campanella, Claudia; Bocca, Beatrice; Petrucci, Francesco; Alimonti, Alessandro; Mizzoni, Davide; Di Raimo, Rossella; Falchi, Mario; Angelini, Daniela F.; Bavisotto, Celeste Caruso; Caimi, Stefano; Bucchieri, Fabio; Logozzi, Mariantonia; Fais, Stefano; Battistini, Luca; Cappello, Francesco; David, Sabrina.

In: Default journal, Vol. 137, 2019, pag. 23-36.

Risultato della ricerca: Article

David, S, Bucchieri, F, Cappello, F, Caruso Bavisotto, C, Campanella, C, Campanella, C, Bocca, B, Petrucci, F, Alimonti, A, Mizzoni, D, Di Raimo, R, Falchi, M, Angelini, DF, Bavisotto, CC, Caimi, S, Bucchieri, F, Logozzi, M, Fais, S, Battistini, L, Cappello, F & David, S 2019, 'Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials', Default journal, vol. 137, pagg. 23-36.
David, Sabrina ; Bucchieri, Fabio ; Cappello, Francesco ; Caruso Bavisotto, Celeste ; Campanella, Claudia ; Campanella, Claudia ; Bocca, Beatrice ; Petrucci, Francesco ; Alimonti, Alessandro ; Mizzoni, Davide ; Di Raimo, Rossella ; Falchi, Mario ; Angelini, Daniela F. ; Bavisotto, Celeste Caruso ; Caimi, Stefano ; Bucchieri, Fabio ; Logozzi, Mariantonia ; Fais, Stefano ; Battistini, Luca ; Cappello, Francesco ; David, Sabrina. / Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials. In: Default journal. 2019 ; Vol. 137. pagg. 23-36.
@article{3310ccb2c9444c4bbac75ce75eb03f6f,
title = "Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials",
abstract = "The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.",
keywords = "Biotechnology, Exosome, NTA, Nanoparticles, Nanotechnology, SP-ICP-MS",
author = "Sabrina David and Fabio Bucchieri and Francesco Cappello and {Caruso Bavisotto}, Celeste and Claudia Campanella and Claudia Campanella and Beatrice Bocca and Francesco Petrucci and Alessandro Alimonti and Davide Mizzoni and {Di Raimo}, Rossella and Mario Falchi and Angelini, {Daniela F.} and Bavisotto, {Celeste Caruso} and Stefano Caimi and Fabio Bucchieri and Mariantonia Logozzi and Stefano Fais and Luca Battistini and Francesco Cappello and Sabrina David",
year = "2019",
language = "English",
volume = "137",
pages = "23--36",
journal = "Default journal",

}

TY - JOUR

T1 - Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials

AU - David, Sabrina

AU - Bucchieri, Fabio

AU - Cappello, Francesco

AU - Caruso Bavisotto, Celeste

AU - Campanella, Claudia

AU - Campanella, Claudia

AU - Bocca, Beatrice

AU - Petrucci, Francesco

AU - Alimonti, Alessandro

AU - Mizzoni, Davide

AU - Di Raimo, Rossella

AU - Falchi, Mario

AU - Angelini, Daniela F.

AU - Bavisotto, Celeste Caruso

AU - Caimi, Stefano

AU - Bucchieri, Fabio

AU - Logozzi, Mariantonia

AU - Fais, Stefano

AU - Battistini, Luca

AU - Cappello, Francesco

AU - David, Sabrina

PY - 2019

Y1 - 2019

N2 - The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.

AB - The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.

KW - Biotechnology

KW - Exosome

KW - NTA

KW - Nanoparticles

KW - Nanotechnology

KW - SP-ICP-MS

UR - http://hdl.handle.net/10447/350848

UR - http://www.elsevier.com/locate/ejphabio

M3 - Article

VL - 137

SP - 23

EP - 36

JO - Default journal

JF - Default journal

ER -