Abstract
Lingua originale | English |
---|---|
pagine (da-a) | 23-36 |
Numero di pagine | 14 |
Rivista | Default journal |
Volume | 137 |
Stato di pubblicazione | Published - 2019 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Pharmaceutical Science
Cita questo
Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials. / David, Sabrina; Bucchieri, Fabio; Cappello, Francesco; Caruso Bavisotto, Celeste; Campanella, Claudia; Campanella, Claudia; Bocca, Beatrice; Petrucci, Francesco; Alimonti, Alessandro; Mizzoni, Davide; Di Raimo, Rossella; Falchi, Mario; Angelini, Daniela F.; Bavisotto, Celeste Caruso; Caimi, Stefano; Bucchieri, Fabio; Logozzi, Mariantonia; Fais, Stefano; Battistini, Luca; Cappello, Francesco; David, Sabrina.
In: Default journal, Vol. 137, 2019, pag. 23-36.Risultato della ricerca: Article
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TY - JOUR
T1 - Human primary macrophages scavenge AuNPs and eliminate it through exosomes. A natural shuttling for nanomaterials
AU - David, Sabrina
AU - Bucchieri, Fabio
AU - Cappello, Francesco
AU - Caruso Bavisotto, Celeste
AU - Campanella, Claudia
AU - Campanella, Claudia
AU - Bocca, Beatrice
AU - Petrucci, Francesco
AU - Alimonti, Alessandro
AU - Mizzoni, Davide
AU - Di Raimo, Rossella
AU - Falchi, Mario
AU - Angelini, Daniela F.
AU - Bavisotto, Celeste Caruso
AU - Caimi, Stefano
AU - Bucchieri, Fabio
AU - Logozzi, Mariantonia
AU - Fais, Stefano
AU - Battistini, Luca
AU - Cappello, Francesco
AU - David, Sabrina
PY - 2019
Y1 - 2019
N2 - The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.
AB - The use of nanomaterials is increasing but the real risk associated with their use in humans has to be defined. In fact, nanomaterials tend to accumulate in organs over a long period of time and are slowly degraded or eliminated by the body. Exosomes are nanovesicles actively shuttle molecules, including chemical products and metals, through the body. Macrophages scavenge the body from both organic and inorganic substances, and they use to release high amounts of exosomes. We hypothesized that macrophages may have a role in eliminating nanomaterials through their exosomes. We treated human primary macrophages with 20 nm gold nanoparticles (AuNPs), analyzing the presence of AuNPs in both cells and the released exosomes by the implementation of different techniques, including SP-ICP-MS and NTA. We showed that macrophages endocytosed AuNPs and released them through exosomes. Our study on one hand provide the evidence for a new methodology in the early identification of the nanomaterials levels in exposed subjects. On the other hand we depict a way our body shuttle virtually intact nanoparticles through macrophage-released exosomes.
KW - Biotechnology
KW - Exosome
KW - NTA
KW - Nanoparticles
KW - Nanotechnology
KW - SP-ICP-MS
UR - http://hdl.handle.net/10447/350848
UR - http://www.elsevier.com/locate/ejphabio
M3 - Article
VL - 137
SP - 23
EP - 36
JO - Default journal
JF - Default journal
ER -