Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations.

Claudia Campanella, Giovanni Zummo, Antonella Marino Gammazza, Giosalba Burgio, Davide Corona, Antonio Palumbo Piccionello, Francesco Cappello, Maria Grazia Ortore, Silvia Vilasi, Claudia Campanella, Rita Carrotta, Antonella Marino Gammazza, Annalisa Vilasi, Fabio Librizzi, Loredana Randazzo, Pier Luigi San Biagio, Silvia Vilasi, Maria Rosalia Mangione, Gabriella Pocsfalvi, Vincenzo MartoranaDonatella Bulone, Everly Conway De Macario, Francesco Cappello, Alberto J. L. Macario, Antonio Palumbo Piccionello

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22 Citazioni (Scopus)

Abstract

It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.
Lingua originaleEnglish
Numero di pagine10
RivistaPLoS One
Volume9
Stato di pubblicazionePublished - 2014

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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Campanella, C., Zummo, G., Marino Gammazza, A., Burgio, G., Corona, D., Palumbo Piccionello, A., Cappello, F., Ortore, M. G., Vilasi, S., Campanella, C., Carrotta, R., Gammazza, A. M., Vilasi, A., Librizzi, F., Randazzo, L., San Biagio, P. L., Vilasi, S., Mangione, M. R., Pocsfalvi, G., ... Piccionello, A. P. (2014). Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations. PLoS One, 9.