TY - JOUR
T1 - Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations.
AU - Campanella, Claudia
AU - Corona, Davide
AU - Zummo, Giovanni
AU - Burgio, Giosalba
AU - Cappello, Francesco
AU - Palumbo Piccionello, Antonio
AU - Marino Gammazza, Antonella
AU - Ortore, Maria Grazia
AU - Vilasi, Silvia
AU - Campanella, Claudia
AU - Carrotta, Rita
AU - Gammazza, Antonella Marino
AU - Vilasi, Annalisa
AU - Librizzi, Fabio
AU - Randazzo, Loredana
AU - San Biagio, Pier Luigi
AU - Vilasi, Silvia
AU - Mangione, Maria Rosalia
AU - Pocsfalvi, Gabriella
AU - Martorana, Vincenzo
AU - Bulone, Donatella
AU - De Macario, Everly Conway
AU - Cappello, Francesco
AU - De Macario, Everly Conway
AU - Macario, Alberto J. L.
AU - Piccionello, Antonio Palumbo
PY - 2014
Y1 - 2014
N2 - It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.
AB - It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.
UR - http://hdl.handle.net/10447/97652
M3 - Article
SN - 1932-6203
VL - 9
JO - PLoS One
JF - PLoS One
ER -