Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations.

Claudia Campanella, Giovanni Zummo, Antonio Palumbo Piccionello, Antonella Marino Gammazza, Francesco Cappello, Davide Corona, Giosalba Burgio, Maria Grazia Ortore, Silvia Vilasi, Claudia Campanella, Rita Carrotta, Antonella Marino Gammazza, Annalisa Vilasi, Fabio Librizzi, Loredana Randazzo, Pier Luigi San Biagio, Maria Rosalia Mangione, Gabriella Pocsfalvi, Vincenzo Martorana, Donatella BuloneEverly Conway De Macario, Francesco Cappello, Alberto J. L. Macario, Antonio Palumbo Piccionello

Risultato della ricerca: Article

22 Citazioni (Scopus)

Abstract

It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.
Lingua originaleEnglish
Numero di pagine10
RivistaPLoS One
Volume9
Stato di pubblicazionePublished - 2014

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imports
cytosol
hydrodynamics
aqueous solutions
spectroscopy
X-radiation
Molecules
Hydrodynamics
X ray scattering
Cytosol
neoplasms
Assays
assays
Chronic Disease
Monomers
X-Rays
Spectroscopy
Light
Neoplasms
methodology

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cita questo

Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations. / Campanella, Claudia; Zummo, Giovanni; Palumbo Piccionello, Antonio; Marino Gammazza, Antonella; Cappello, Francesco; Corona, Davide; Burgio, Giosalba; Ortore, Maria Grazia; Vilasi, Silvia; Campanella, Claudia; Carrotta, Rita; Gammazza, Antonella Marino; Vilasi, Annalisa; Librizzi, Fabio; Randazzo, Loredana; San Biagio, Pier Luigi; Mangione, Maria Rosalia; Pocsfalvi, Gabriella; Martorana, Vincenzo; Bulone, Donatella; De Macario, Everly Conway; Cappello, Francesco; Macario, Alberto J. L.; Piccionello, Antonio Palumbo.

In: PLoS One, Vol. 9, 2014.

Risultato della ricerca: Article

Campanella, C, Zummo, G, Palumbo Piccionello, A, Marino Gammazza, A, Cappello, F, Corona, D, Burgio, G, Ortore, MG, Vilasi, S, Campanella, C, Carrotta, R, Gammazza, AM, Vilasi, A, Librizzi, F, Randazzo, L, San Biagio, PL, Mangione, MR, Pocsfalvi, G, Martorana, V, Bulone, D, De Macario, EC, Cappello, F, Macario, AJL & Piccionello, AP 2014, 'Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations.', PLoS One, vol. 9.
Campanella, Claudia ; Zummo, Giovanni ; Palumbo Piccionello, Antonio ; Marino Gammazza, Antonella ; Cappello, Francesco ; Corona, Davide ; Burgio, Giosalba ; Ortore, Maria Grazia ; Vilasi, Silvia ; Campanella, Claudia ; Carrotta, Rita ; Gammazza, Antonella Marino ; Vilasi, Annalisa ; Librizzi, Fabio ; Randazzo, Loredana ; San Biagio, Pier Luigi ; Mangione, Maria Rosalia ; Pocsfalvi, Gabriella ; Martorana, Vincenzo ; Bulone, Donatella ; De Macario, Everly Conway ; Cappello, Francesco ; Macario, Alberto J. L. ; Piccionello, Antonio Palumbo. / Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations. In: PLoS One. 2014 ; Vol. 9.
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title = "Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations.",
abstract = "It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be na{\"i}ve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of na{\"i}ve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Na{\"i}ve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.",
author = "Claudia Campanella and Giovanni Zummo and {Palumbo Piccionello}, Antonio and {Marino Gammazza}, Antonella and Francesco Cappello and Davide Corona and Giosalba Burgio and Ortore, {Maria Grazia} and Silvia Vilasi and Claudia Campanella and Rita Carrotta and Gammazza, {Antonella Marino} and Annalisa Vilasi and Fabio Librizzi and Loredana Randazzo and {San Biagio}, {Pier Luigi} and Mangione, {Maria Rosalia} and Gabriella Pocsfalvi and Vincenzo Martorana and Donatella Bulone and {De Macario}, {Everly Conway} and Francesco Cappello and Macario, {Alberto J. L.} and Piccionello, {Antonio Palumbo}",
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TY - JOUR

T1 - Human Hsp60 with its mitochondrial import signaloccurs in solution as heptamers and tetradecamers remarkably stable over a widerange of concentrations.

AU - Campanella, Claudia

AU - Zummo, Giovanni

AU - Palumbo Piccionello, Antonio

AU - Marino Gammazza, Antonella

AU - Cappello, Francesco

AU - Corona, Davide

AU - Burgio, Giosalba

AU - Ortore, Maria Grazia

AU - Vilasi, Silvia

AU - Campanella, Claudia

AU - Carrotta, Rita

AU - Gammazza, Antonella Marino

AU - Vilasi, Annalisa

AU - Librizzi, Fabio

AU - Randazzo, Loredana

AU - San Biagio, Pier Luigi

AU - Mangione, Maria Rosalia

AU - Pocsfalvi, Gabriella

AU - Martorana, Vincenzo

AU - Bulone, Donatella

AU - De Macario, Everly Conway

AU - Cappello, Francesco

AU - Macario, Alberto J. L.

AU - Piccionello, Antonio Palumbo

PY - 2014

Y1 - 2014

N2 - It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.

AB - It has been established that Hsp60 can accumulate in the cytosol in various pathological conditions, including cancer and chronic inflammatory diseases. Part or all of the cytosolic Hsp60 could be naïve, namely, bear the mitochondrial import signal (MIS), but neither the structure nor the in solution oligomeric organization of this cytosolic molecule has still been elucidated. Here we present a detailed study of the structure and self-organization of naïve cytosolic Hsp60 in solution. Results were obtained by different biophysical methods (light and X ray scattering, single molecule spectroscopy and hydrodynamics) that all together allowed us to assay a wide range of concentrations of Hsp60. We found that Naïve Hsp60 in aqueous solution is assembled in very stable heptamers and tetradecamers at all concentrations assayed, without any trace of monomer presence.

UR - http://hdl.handle.net/10447/97652

M3 - Article

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

ER -