Human fetal aorta contains vascular progenitor cells capable of inducingvasculogenesis, angiogenesis, and myogenesis in vitro and in a murine model ofperipheral ischemia.

Giorgio Stassi, Gloria Invernici, Sergio Gadau, Augusto Colombo, Umberto Fascio, Tommaso Rizzuti, Emilio Ciusani, Anna Benetti, Eugenio Parati, Giulio Alessandri, Costanza Emanueli, Roberto Nicosia, Paolo Madeddu, Cesare Peschle, Silvia Cristini

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Abstract

Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (ie, angioblasts), is poorly understood. We report the identification of a population of vascular progenitor cells (hVPCs) in the human fetal aorta composed of undifferentiated mesenchymal cells that coexpress endothelial and myogenic markers. Under culture conditions that promoted cell differentiation, hVPCs gave rise to a mixed population of mature endothelial and mural cells when progenitor cells were stimulated with vascular endothelial growth factor-A or platelet-derived growth factor-ββ. hVPCs grew as nonadherent cells and, when embedded in a three-dimensional collagen gel, reorganized into cohesive cellular cords that resembled mature vascular structures. hVPC-conditioned medium contained angiogenic substances (vascular endothelial growth factor-A and angiopoietin-2) and strongly stimulated the proliferation of endothelial cells. We also demonstrate the therapeutic efficacy of a small number of hVPCs transplanted into ischemic limb muscle of immunodeficient mice. hVPCs markedly improved neovascularization and inhibited the loss of endogenous endothelial cells and myocytes, thus ameliorating the clinical outcome from ischemia. We conclude that fetal aorta represents an important source for the investigation of the phenotypic and functional features of human vascular progenitor cells.
Lingua originaleEnglish
pagine (da-a)1879-1892
RivistaTHE AMERICAN JOURNAL OF PATHOLOGY
Volume170(6)
Stato di pubblicazionePublished - 2007

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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    Stassi, G., Invernici, G., Gadau, S., Colombo, A., Fascio, U., Rizzuti, T., Ciusani, E., Benetti, A., Parati, E., Alessandri, G., Emanueli, C., Nicosia, R., Madeddu, P., Peschle, C., & Cristini, S. (2007). Human fetal aorta contains vascular progenitor cells capable of inducingvasculogenesis, angiogenesis, and myogenesis in vitro and in a murine model ofperipheral ischemia. THE AMERICAN JOURNAL OF PATHOLOGY, 170(6), 1879-1892.