Human CD4 T-cells with a naive phenotype produce multiple cytokines during Mycobacterium tuberculosis infection and correlate with active disease

Marco Pio La Manna, Carmela La Mendola, Francesco Dieli, Elena Lo Presti, Nadia Rosalia Caccamo, Simona Buccheri, Simone A. Joosten, Delia Goletti, Tom H.M. Ottenhoff, Fabrizio Palmieri, Simona Buccheri, Valentina Orlando

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

T-cell-mediated immune responses play a fundamental role in controlling Mycobacterium tuberculosis (M. tuberculosis) infection, and traditionally, this response is thought to be mediated by Th1-type CD4+ T-cells secreting IFN-γ. While studying the function and specificity of M. tuberculosis-reactive CD4+ T-cells in more detail at the single cell level; however, we found a human CD4+ T-cell population with a naive phenotype that interestingly was capable of producing multiple cytokines (TCNP cells). CD4+ TCNP cells phenotyped as CD95lo CD28int CD49dhi CXCR3hi and showed a broad distribution of T cell receptor Vβ segments. They rapidly secreted multiple cytokines in response to different M. tuberculosis antigens, their frequency was increased during active disease, but was comparable to latent tuberculosis infection in treated TB patients. These results identify a novel human CD4+ T-cell subset involved in the human immune response to mycobacteria, which is present in active TB patients' blood. These results significantly expand our understanding of the immune response in infectious diseases.
Lingua originaleEnglish
pagine (da-a)1119-
Numero di pagine0
RivistaFrontiers in Immunology
Volume9
Stato di pubblicazionePublished - 2018

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Mycobacterium Infections
triciribine phosphate
Mycobacterium tuberculosis
Cytokines
T-Lymphocytes
Phenotype
Latent Tuberculosis
T-Lymphocyte Subsets
Mycobacterium
T-Cell Antigen Receptor
Communicable Diseases
Population

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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Human CD4 T-cells with a naive phenotype produce multiple cytokines during Mycobacterium tuberculosis infection and correlate with active disease. / La Manna, Marco Pio; La Mendola, Carmela; Dieli, Francesco; Lo Presti, Elena; Caccamo, Nadia Rosalia; Buccheri, Simona; Joosten, Simone A.; Goletti, Delia; Ottenhoff, Tom H.M.; Palmieri, Fabrizio; Buccheri, Simona; Orlando, Valentina.

In: Frontiers in Immunology, Vol. 9, 2018, pag. 1119-.

Risultato della ricerca: Article

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title = "Human CD4 T-cells with a naive phenotype produce multiple cytokines during Mycobacterium tuberculosis infection and correlate with active disease",
abstract = "T-cell-mediated immune responses play a fundamental role in controlling Mycobacterium tuberculosis (M. tuberculosis) infection, and traditionally, this response is thought to be mediated by Th1-type CD4+ T-cells secreting IFN-γ. While studying the function and specificity of M. tuberculosis-reactive CD4+ T-cells in more detail at the single cell level; however, we found a human CD4+ T-cell population with a naive phenotype that interestingly was capable of producing multiple cytokines (TCNP cells). CD4+ TCNP cells phenotyped as CD95lo CD28int CD49dhi CXCR3hi and showed a broad distribution of T cell receptor Vβ segments. They rapidly secreted multiple cytokines in response to different M. tuberculosis antigens, their frequency was increased during active disease, but was comparable to latent tuberculosis infection in treated TB patients. These results identify a novel human CD4+ T-cell subset involved in the human immune response to mycobacteria, which is present in active TB patients' blood. These results significantly expand our understanding of the immune response in infectious diseases.",
keywords = "CD4 T-cells, Cytokines, Immunology, Immunology and Allergy, Mycobacterium tuberculosis, Naive cells, Tuberculosis",
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T1 - Human CD4 T-cells with a naive phenotype produce multiple cytokines during Mycobacterium tuberculosis infection and correlate with active disease

AU - La Manna, Marco Pio

AU - La Mendola, Carmela

AU - Dieli, Francesco

AU - Lo Presti, Elena

AU - Caccamo, Nadia Rosalia

AU - Buccheri, Simona

AU - Joosten, Simone A.

AU - Goletti, Delia

AU - Ottenhoff, Tom H.M.

AU - Palmieri, Fabrizio

AU - Buccheri, Simona

AU - Orlando, Valentina

PY - 2018

Y1 - 2018

N2 - T-cell-mediated immune responses play a fundamental role in controlling Mycobacterium tuberculosis (M. tuberculosis) infection, and traditionally, this response is thought to be mediated by Th1-type CD4+ T-cells secreting IFN-γ. While studying the function and specificity of M. tuberculosis-reactive CD4+ T-cells in more detail at the single cell level; however, we found a human CD4+ T-cell population with a naive phenotype that interestingly was capable of producing multiple cytokines (TCNP cells). CD4+ TCNP cells phenotyped as CD95lo CD28int CD49dhi CXCR3hi and showed a broad distribution of T cell receptor Vβ segments. They rapidly secreted multiple cytokines in response to different M. tuberculosis antigens, their frequency was increased during active disease, but was comparable to latent tuberculosis infection in treated TB patients. These results identify a novel human CD4+ T-cell subset involved in the human immune response to mycobacteria, which is present in active TB patients' blood. These results significantly expand our understanding of the immune response in infectious diseases.

AB - T-cell-mediated immune responses play a fundamental role in controlling Mycobacterium tuberculosis (M. tuberculosis) infection, and traditionally, this response is thought to be mediated by Th1-type CD4+ T-cells secreting IFN-γ. While studying the function and specificity of M. tuberculosis-reactive CD4+ T-cells in more detail at the single cell level; however, we found a human CD4+ T-cell population with a naive phenotype that interestingly was capable of producing multiple cytokines (TCNP cells). CD4+ TCNP cells phenotyped as CD95lo CD28int CD49dhi CXCR3hi and showed a broad distribution of T cell receptor Vβ segments. They rapidly secreted multiple cytokines in response to different M. tuberculosis antigens, their frequency was increased during active disease, but was comparable to latent tuberculosis infection in treated TB patients. These results identify a novel human CD4+ T-cell subset involved in the human immune response to mycobacteria, which is present in active TB patients' blood. These results significantly expand our understanding of the immune response in infectious diseases.

KW - CD4 T-cells

KW - Cytokines

KW - Immunology

KW - Immunology and Allergy

KW - Mycobacterium tuberculosis

KW - Naive cells

KW - Tuberculosis

UR - http://hdl.handle.net/10447/358970

UR - https://www.frontiersin.org/articles/10.3389/fimmu.2018.01119/full

M3 - Article

VL - 9

SP - 1119-

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

ER -