Abstract

The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.
Lingua originaleEnglish
Numero di pagine0
RivistaCELL STRESS & CHAPERONES
Volume2010
Stato di pubblicazionePublished - 2010

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Chaperonins
Ulcerative Colitis
Crohn Disease
Colon
Mucous Membrane
Inflammation
Western Blotting
Epithelial Cells
Connective Tissue Cells
Inflammatory Bowel Diseases
Fluorescent Antibody Technique
Immune System
Cytoplasm
Immune system
Immunohistochemistry
Recurrence
Chemical activation
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology

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@article{31316e80dede46e8b0b486be529d4190,
title = "Hsp60 and Hsp10 increase in colon mucosa of Crohn's disease and ulcerative colitis.",
abstract = "The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.",
keywords = "HSP 10, HSP 60, IBD",
author = "Monica Zerilli and Giovanni Zummo and Salvatore Accomando and Anna Martorana and Alessandro Pitruzzella and Vito Rodolico and {Marino Gammazza}, Antonella and Giovanni Tomasello and Sabrina David and Francesco Cappello and Francesco Cappello and {Conway De Macario}, Everly and Macario, {Alberto J. L.}",
year = "2010",
language = "English",
volume = "2010",
journal = "CELL STRESS & CHAPERONES",
issn = "1355-8145",

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TY - JOUR

T1 - Hsp60 and Hsp10 increase in colon mucosa of Crohn's disease and ulcerative colitis.

AU - Zerilli, Monica

AU - Zummo, Giovanni

AU - Accomando, Salvatore

AU - Martorana, Anna

AU - Pitruzzella, Alessandro

AU - Rodolico, Vito

AU - Marino Gammazza, Antonella

AU - Tomasello, Giovanni

AU - David, Sabrina

AU - Cappello, Francesco

AU - Cappello, Francesco

AU - Conway De Macario, Everly

AU - Macario, Alberto J. L.

PY - 2010

Y1 - 2010

N2 - The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.

AB - The purpose of this work was to determine in colon mucosa of Crohn’s disease (CD) and ulcerative colitis (UC) in relapse: a) the levels of the chaperonins Hsp60 and Hsp10; b) the quantity of inflammatory cells; and c) if the levels of chaperonins parallel those of inflammation cells. Twenty cases of CD and UC and twenty normal controls (NC) were studied using immunohistochemistry, Western blotting and immunofluorescence. Immunohistochemically, Hsp60 and Hsp10 were increased in both inflammatory bowel diseases (IBD) compared to NC. These results were confirmed by Western blotting. Hsp60 and Hsp10 occurred in the cytoplasm of epithelial cells in CD and UC but not in NC. Hsp60 and Hsp10 co-localised to epithelial cells of mucosal glands but not always in connective tissue cells of lamina propria, where only Hsp60 or, less often, Hsp10 was found. Cells typical of inflammation were significantly more abundant in CD and UC than in NC. Since chaperonins are key factors in the activation of the immune system leading to inflammation, we propose that they play a central role in the pathogenesis of the two diseases, which, consequently, ought to be studied as chaperonopathies.

KW - HSP 10

KW - HSP 60

KW - IBD

UR - http://hdl.handle.net/10447/49474

M3 - Article

VL - 2010

JO - CELL STRESS & CHAPERONES

JF - CELL STRESS & CHAPERONES

SN - 1355-8145

ER -