HSP60 and HSP10 down-regulation predicts bronchial epithelial carcinogenesis in smokers with chronic obstructive pulmonary disease

Francesco Cappello, Sabrina David, Rita Anzalone, Francesca Rappa, Giampiero La Rocca, Giovanni Zummo, Antonino Di Stefano, Bruno Balbi, Claudio F. Donner, Francesca Magno, Silvestro E. D'Anna, Francesca Magno, Salvatore D'Anna

Risultato della ricerca: Article

70 Citazioni (Scopus)

Abstract

BACKGROUND. The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequencyof adenocarcinoma has been reported in patients with COPD. Heat shockproteins (Hsps) are implicated in tumoral cell growth and differentiation. The aimof the present study was to investigate the expression of Hsp60 and Hsp10 in bronchialbiopsies from smokers with COPD and in 10 lung cancer patients and to evaluate the association between Hsps expression and carcinogenetic steps of LC. METHODS. An immunohistochemical study was performed for Hsp60 and Hsp10 in bronchial biopsies from 35 COPD (postbronchodilator forced expiratory volume in 1 second [FEV1]: 53±19% [mean ± SD]) patients with a history of smoking (53±34 pack/years) and in 10 patients with adenocarcinoma or adenosquamous carcinoma (ASC). Immunopositivity was quantified in the bronchial epithelium and in specimens with ASC. RESULTS. In smokers with COPD, 10 out of 35 patients had a normal bronchial epithelium (NBE), 12 showed basal cell hyperplasia (BCH), 5 squamous metaplasia(SM), and 8 dysplasia (Dy). It was found that 58 ± 23% and 54 ± 23% of NBE and48 ± 29% and 52 ± 26% of BCH expressed Hsp60 and Hsp10, respectively; in contrast, only 3 ± 3% and 3.6 ± 2% of SM, 1.9 ± 4% and 1.1 ± 2% of Dy expressed Hsp60 and Hsp10, respectively. ASC specimens were negative for Hsps proteins. Interestingly, NBE also present at the edges of ASC specimens was negative for Hsps proteins.CONCLUSIONS. The loss of Hsp60 and Hsp10 immunopositivity is related to the development and progression of bronchial cancer in smokers with COPD.
Lingua originaleEnglish
pagine (da-a)2417-2424
Numero di pagine8
RivistaCancer
Volume107
Stato di pubblicazionePublished - 2006

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Chronic Obstructive Pulmonary Disease
Adenosquamous Carcinoma
Carcinogenesis
Down-Regulation
Lung Neoplasms
Epithelium
Hot Temperature
Metaplasia
Hyperplasia
Adenocarcinoma
Smoking
Forced Expiratory Volume
Cell Differentiation
Proteins
Biopsy
Growth
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cita questo

HSP60 and HSP10 down-regulation predicts bronchial epithelial carcinogenesis in smokers with chronic obstructive pulmonary disease. / Cappello, Francesco; David, Sabrina; Anzalone, Rita; Rappa, Francesca; La Rocca, Giampiero; Zummo, Giovanni; Di Stefano, Antonino; Balbi, Bruno; Donner, Claudio F.; Magno, Francesca; D'Anna, Silvestro E.; Magno, Francesca; D'Anna, Salvatore.

In: Cancer, Vol. 107, 2006, pag. 2417-2424.

Risultato della ricerca: Article

Cappello, Francesco ; David, Sabrina ; Anzalone, Rita ; Rappa, Francesca ; La Rocca, Giampiero ; Zummo, Giovanni ; Di Stefano, Antonino ; Balbi, Bruno ; Donner, Claudio F. ; Magno, Francesca ; D'Anna, Silvestro E. ; Magno, Francesca ; D'Anna, Salvatore. / HSP60 and HSP10 down-regulation predicts bronchial epithelial carcinogenesis in smokers with chronic obstructive pulmonary disease. In: Cancer. 2006 ; Vol. 107. pagg. 2417-2424.
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title = "HSP60 and HSP10 down-regulation predicts bronchial epithelial carcinogenesis in smokers with chronic obstructive pulmonary disease",
abstract = "BACKGROUND. The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequencyof adenocarcinoma has been reported in patients with COPD. Heat shockproteins (Hsps) are implicated in tumoral cell growth and differentiation. The aimof the present study was to investigate the expression of Hsp60 and Hsp10 in bronchialbiopsies from smokers with COPD and in 10 lung cancer patients and to evaluate the association between Hsps expression and carcinogenetic steps of LC. METHODS. An immunohistochemical study was performed for Hsp60 and Hsp10 in bronchial biopsies from 35 COPD (postbronchodilator forced expiratory volume in 1 second [FEV1]: 53±19{\%} [mean ± SD]) patients with a history of smoking (53±34 pack/years) and in 10 patients with adenocarcinoma or adenosquamous carcinoma (ASC). Immunopositivity was quantified in the bronchial epithelium and in specimens with ASC. RESULTS. In smokers with COPD, 10 out of 35 patients had a normal bronchial epithelium (NBE), 12 showed basal cell hyperplasia (BCH), 5 squamous metaplasia(SM), and 8 dysplasia (Dy). It was found that 58 ± 23{\%} and 54 ± 23{\%} of NBE and48 ± 29{\%} and 52 ± 26{\%} of BCH expressed Hsp60 and Hsp10, respectively; in contrast, only 3 ± 3{\%} and 3.6 ± 2{\%} of SM, 1.9 ± 4{\%} and 1.1 ± 2{\%} of Dy expressed Hsp60 and Hsp10, respectively. ASC specimens were negative for Hsps proteins. Interestingly, NBE also present at the edges of ASC specimens was negative for Hsps proteins.CONCLUSIONS. The loss of Hsp60 and Hsp10 immunopositivity is related to the development and progression of bronchial cancer in smokers with COPD.",
keywords = "chaperone expression, lung obstruction, lung tumors, smoking",
author = "Francesco Cappello and Sabrina David and Rita Anzalone and Francesca Rappa and {La Rocca}, Giampiero and Giovanni Zummo and {Di Stefano}, Antonino and Bruno Balbi and Donner, {Claudio F.} and Francesca Magno and D'Anna, {Silvestro E.} and Francesca Magno and Salvatore D'Anna",
year = "2006",
language = "English",
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journal = "Cancer",
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TY - JOUR

T1 - HSP60 and HSP10 down-regulation predicts bronchial epithelial carcinogenesis in smokers with chronic obstructive pulmonary disease

AU - Cappello, Francesco

AU - David, Sabrina

AU - Anzalone, Rita

AU - Rappa, Francesca

AU - La Rocca, Giampiero

AU - Zummo, Giovanni

AU - Di Stefano, Antonino

AU - Balbi, Bruno

AU - Donner, Claudio F.

AU - Magno, Francesca

AU - D'Anna, Silvestro E.

AU - Magno, Francesca

AU - D'Anna, Salvatore

PY - 2006

Y1 - 2006

N2 - BACKGROUND. The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequencyof adenocarcinoma has been reported in patients with COPD. Heat shockproteins (Hsps) are implicated in tumoral cell growth and differentiation. The aimof the present study was to investigate the expression of Hsp60 and Hsp10 in bronchialbiopsies from smokers with COPD and in 10 lung cancer patients and to evaluate the association between Hsps expression and carcinogenetic steps of LC. METHODS. An immunohistochemical study was performed for Hsp60 and Hsp10 in bronchial biopsies from 35 COPD (postbronchodilator forced expiratory volume in 1 second [FEV1]: 53±19% [mean ± SD]) patients with a history of smoking (53±34 pack/years) and in 10 patients with adenocarcinoma or adenosquamous carcinoma (ASC). Immunopositivity was quantified in the bronchial epithelium and in specimens with ASC. RESULTS. In smokers with COPD, 10 out of 35 patients had a normal bronchial epithelium (NBE), 12 showed basal cell hyperplasia (BCH), 5 squamous metaplasia(SM), and 8 dysplasia (Dy). It was found that 58 ± 23% and 54 ± 23% of NBE and48 ± 29% and 52 ± 26% of BCH expressed Hsp60 and Hsp10, respectively; in contrast, only 3 ± 3% and 3.6 ± 2% of SM, 1.9 ± 4% and 1.1 ± 2% of Dy expressed Hsp60 and Hsp10, respectively. ASC specimens were negative for Hsps proteins. Interestingly, NBE also present at the edges of ASC specimens was negative for Hsps proteins.CONCLUSIONS. The loss of Hsp60 and Hsp10 immunopositivity is related to the development and progression of bronchial cancer in smokers with COPD.

AB - BACKGROUND. The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequencyof adenocarcinoma has been reported in patients with COPD. Heat shockproteins (Hsps) are implicated in tumoral cell growth and differentiation. The aimof the present study was to investigate the expression of Hsp60 and Hsp10 in bronchialbiopsies from smokers with COPD and in 10 lung cancer patients and to evaluate the association between Hsps expression and carcinogenetic steps of LC. METHODS. An immunohistochemical study was performed for Hsp60 and Hsp10 in bronchial biopsies from 35 COPD (postbronchodilator forced expiratory volume in 1 second [FEV1]: 53±19% [mean ± SD]) patients with a history of smoking (53±34 pack/years) and in 10 patients with adenocarcinoma or adenosquamous carcinoma (ASC). Immunopositivity was quantified in the bronchial epithelium and in specimens with ASC. RESULTS. In smokers with COPD, 10 out of 35 patients had a normal bronchial epithelium (NBE), 12 showed basal cell hyperplasia (BCH), 5 squamous metaplasia(SM), and 8 dysplasia (Dy). It was found that 58 ± 23% and 54 ± 23% of NBE and48 ± 29% and 52 ± 26% of BCH expressed Hsp60 and Hsp10, respectively; in contrast, only 3 ± 3% and 3.6 ± 2% of SM, 1.9 ± 4% and 1.1 ± 2% of Dy expressed Hsp60 and Hsp10, respectively. ASC specimens were negative for Hsps proteins. Interestingly, NBE also present at the edges of ASC specimens was negative for Hsps proteins.CONCLUSIONS. The loss of Hsp60 and Hsp10 immunopositivity is related to the development and progression of bronchial cancer in smokers with COPD.

KW - chaperone expression

KW - lung obstruction

KW - lung tumors

KW - smoking

UR - http://hdl.handle.net/10447/39770

UR - http://www3.interscience.wiley.com/cgi-bin/fulltext/113399511/PDFSTART

M3 - Article

VL - 107

SP - 2417

EP - 2424

JO - Cancer

JF - Cancer

SN - 0008-543X

ER -