Hsp60, amateur chaperone in amyloid-beta fibrillogenesis.

Alberto Giuseppe Fucarino, Francesco Cappello, Dario Spigolon, Fabio Bucchieri, Claudia Marino, Silvia Vilasi, Fabio Librizzi, Alberto Fucarino, Dario Spigolon, Claudia Marino, Pier Luigi San Biagio, Silvia Vilasi, Rosa Passantino, Fabio Bucchieri, Maria Rosalia Mangione, Donatella Bulone, Francesco Cappello, Claudio Canale, Carmelinda Canale, Maria Rosalia MangionePier Luigi San Biagio

Risultato della ricerca: Article

17 Citazioni (Scopus)

Abstract

BACKGROUND:Molecular chaperones are a very special class of proteins that play essential roles in many cellular processes like folding, targeting and transport of proteins. Moreover, recent evidence indicates that chaperones can act as potentially strong suppressor agents in Alzheimer's disease (AD). Indeed, in vitro experiments demonstrate that several chaperones are able to significantly slow down or suppress aggregation of Aβ peptide and in vivo studies reveal that treatment with specific chaperones or their overexpression can ameliorate some distinct pathological signs characterizing AD.METHODS:Here we investigate using a biophysical approach (fluorescence, circular dichroism (CD), transmission electron (TEM) and atomic force (AFM) microscopy, size exclusion chromatography (SEC)) the effect of the human chaperonin Hsp60 on Aβ fibrillogenesis.RESULTS:We found that Hsp60 powerfully inhibits Aβ amyloid aggregation, by closing molecular pathways leading to peptide fibrillogenesis.CONCLUSIONS:We observe that Hsp60 inhibits Aβ aggregation through a more complex mechanism than a simple folding chaperone action. The action is specifically directed toward the early oligomeric species behaving as aggregation seeds for on-pathway amyloid fibrillogenesis.GENERAL SIGNIFICANCE:Understanding the specificity of the molecular interactions of Hsp60 with amyloid Aβ peptide allowed us to emphasize the important aspects to be taken into consideration when considering the recent promising therapeutic strategies for neurodegeneration.Copyright © 2016 Elsevier B.V. All rights reserved.
Lingua originaleEnglish
Numero di pagine10
RivistaBIOCHIMICA ET BIOPHYSICA ACTA
Volume1860
Stato di pubblicazionePublished - 2016

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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    Fucarino, A. G., Cappello, F., Spigolon, D., Bucchieri, F., Marino, C., Vilasi, S., Librizzi, F., Fucarino, A., Spigolon, D., Marino, C., San Biagio, P. L., Vilasi, S., Passantino, R., Bucchieri, F., Mangione, M. R., Bulone, D., Cappello, F., Canale, C., Canale, C., ... San Biagio, P. L. (2016). Hsp60, amateur chaperone in amyloid-beta fibrillogenesis. BIOCHIMICA ET BIOPHYSICA ACTA, 1860.