Hsp56 protein and mRNA distribution in normal and stressed P.lividus embryos

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Abstract

It was previously demonstrated that Paracentrotus lividus Hsp56 mitochondrial chaperonin is con-stitutively expressed during development, that it increases after heat-shock and cadmium treatment, and that it has a specific territorial distribution, both in normal and heat-shocked embryos, as shown by immunolocalization experiments. In this work, we analyzed by Western blot the territorial distribution of the protein in plutei exposed to heat-shock or sublethal cadmium concentrations, and we found that Hsp56 increases in both ectodermal and en-dodermal cells. Moreover, by “in situ” hybridization, we looked at Hsp56 mRNA during normal development and under stress conditions. We found that the territorial distribution of the messenger changes during development and that its amount is steadily increased in stressed embryos. Finally, by T1 RNase assay, we identified a cytoplasmic factor that binds to the region of Hsp56 messenger containing the 5’UTR
Lingua originaleEnglish
pagine (da-a)82-87
Numero di pagine6
RivistaCaryologia
Volume61
Stato di pubblicazionePublished - 2008

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1100.1100???

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