Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin.

Rita Anzalone, Tiziana Corsello, Francesco Cappello, Felicia Farina, Giampiero La Rocca, Davide Corona, Simona Corrao, Antonino Di Stefano, Tiziana Corsello, Melania Lo Iacono, Giampiero La Rocca, Anna Maria Timperio, Bruno Balbi, Everly Conway De Macario, Mauro Carone, Lello Zolla, Francesco Cappello, Alberto J. L. Macario, Silvestro Ennio D'Anna, Anna SalaMelania Lo Iacono, Simona Corrao

Risultato della ricerca: Article

12 Citazioni (Scopus)

Abstract

Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.
Lingua originaleEnglish
Numero di pagine0
RivistaOpen Biology
Volume4
Stato di pubblicazionePublished - 2014

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Chaperonins
Smoke
Tobacco Products
Lung
Pulmonary diseases
Chronic Obstructive Pulmonary Disease
Mucous Membrane
Chaperonin 10
Mitochondria
DNA
Isoelectric Point
Fibroblasts
Organelles
Cytosol
Molecular Weight
Molecular weight
Cells
Cell Line
Neoplasms
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Cita questo

Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin. / Anzalone, Rita; Corsello, Tiziana; Cappello, Francesco; Farina, Felicia; La Rocca, Giampiero; Corona, Davide; Corrao, Simona; Stefano, Antonino Di; Corsello, Tiziana; Iacono, Melania Lo; Rocca, Giampiero La; Timperio, Anna Maria; Balbi, Bruno; De Macario, Everly Conway; Carone, Mauro; Zolla, Lello; Cappello, Francesco; Macario, Alberto J. L.; D'Anna, Silvestro Ennio; Sala, Anna; Lo Iacono, Melania; Corrao, Simona.

In: Open Biology, Vol. 4, 2014.

Risultato della ricerca: Article

Anzalone, R, Corsello, T, Cappello, F, Farina, F, La Rocca, G, Corona, D, Corrao, S, Stefano, AD, Corsello, T, Iacono, ML, Rocca, GL, Timperio, AM, Balbi, B, De Macario, EC, Carone, M, Zolla, L, Cappello, F, Macario, AJL, D'Anna, SE, Sala, A, Lo Iacono, M & Corrao, S 2014, 'Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin.', Open Biology, vol. 4.
Anzalone, Rita ; Corsello, Tiziana ; Cappello, Francesco ; Farina, Felicia ; La Rocca, Giampiero ; Corona, Davide ; Corrao, Simona ; Stefano, Antonino Di ; Corsello, Tiziana ; Iacono, Melania Lo ; Rocca, Giampiero La ; Timperio, Anna Maria ; Balbi, Bruno ; De Macario, Everly Conway ; Carone, Mauro ; Zolla, Lello ; Cappello, Francesco ; Macario, Alberto J. L. ; D'Anna, Silvestro Ennio ; Sala, Anna ; Lo Iacono, Melania ; Corrao, Simona. / Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin. In: Open Biology. 2014 ; Vol. 4.
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title = "Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin.",
abstract = "Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.",
author = "Rita Anzalone and Tiziana Corsello and Francesco Cappello and Felicia Farina and {La Rocca}, Giampiero and Davide Corona and Simona Corrao and Stefano, {Antonino Di} and Tiziana Corsello and Iacono, {Melania Lo} and Rocca, {Giampiero La} and Timperio, {Anna Maria} and Bruno Balbi and {De Macario}, {Everly Conway} and Mauro Carone and Lello Zolla and Francesco Cappello and Macario, {Alberto J. L.} and D'Anna, {Silvestro Ennio} and Anna Sala and {Lo Iacono}, Melania and Simona Corrao",
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journal = "Open Biology",
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TY - JOUR

T1 - Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin.

AU - Anzalone, Rita

AU - Corsello, Tiziana

AU - Cappello, Francesco

AU - Farina, Felicia

AU - La Rocca, Giampiero

AU - Corona, Davide

AU - Corrao, Simona

AU - Stefano, Antonino Di

AU - Corsello, Tiziana

AU - Iacono, Melania Lo

AU - Rocca, Giampiero La

AU - Timperio, Anna Maria

AU - Balbi, Bruno

AU - De Macario, Everly Conway

AU - Carone, Mauro

AU - Zolla, Lello

AU - Cappello, Francesco

AU - Macario, Alberto J. L.

AU - D'Anna, Silvestro Ennio

AU - Sala, Anna

AU - Lo Iacono, Melania

AU - Corrao, Simona

PY - 2014

Y1 - 2014

N2 - Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.

AB - Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.

UR - http://hdl.handle.net/10447/102167

UR - http://rsob.royalsocietypublishing.org/content/4/10/140125

M3 - Article

VL - 4

JO - Open Biology

JF - Open Biology

SN - 2046-2441

ER -