Abstract

Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseasescausing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPVinfection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developeda new approach to evaluate virus localisation in the different semen components. We analysedalso the specific genotype localisation and viral DNA quantity by qPCR. Results show thatHPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and severalHPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPVDNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective,suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support theproposed role of HPV in decreased fertility and prompt new possible consequences of theinfection in semen.
Lingua originaleEnglish
pagine (da-a)1-8
Numero di pagine8
RivistaEpidemiology and Infection
Volume147
Stato di pubblicazionePublished - 2019

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Papillomavirus Infections
Semen
Spermatozoa
Genotype
Fertility
Penile Neoplasms
Warts
Viral Genome
DNA
Viral DNA
Uterine Cervical Neoplasms
Genetic Recombination
Viruses
Infection
Neoplasms

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Infectious Diseases

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title = "HPV infection in semen: results from a new molecular approach",
abstract = "Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseasescausing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPVinfection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developeda new approach to evaluate virus localisation in the different semen components. We analysedalso the specific genotype localisation and viral DNA quantity by qPCR. Results show thatHPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and severalHPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPVDNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective,suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support theproposed role of HPV in decreased fertility and prompt new possible consequences of theinfection in semen.",
keywords = "HPV infection, male infertility, semen infection, semen parameters, sexually transmitted diseases, viral infection",
author = "Giuseppina Capra and Liana Bosco and Antonino Perino and Roccheri, {Maria Carmela} and Ragusa, {Maria Antonietta} and Rosaria Schillaci",
year = "2019",
language = "English",
volume = "147",
pages = "1--8",
journal = "Epidemiology and Infection",
issn = "0950-2688",
publisher = "Cambridge University Press",

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TY - JOUR

T1 - HPV infection in semen: results from a new molecular approach

AU - Capra, Giuseppina

AU - Bosco, Liana

AU - Perino, Antonino

AU - Roccheri, Maria Carmela

AU - Ragusa, Maria Antonietta

AU - Schillaci, Rosaria

PY - 2019

Y1 - 2019

N2 - Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseasescausing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPVinfection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developeda new approach to evaluate virus localisation in the different semen components. We analysedalso the specific genotype localisation and viral DNA quantity by qPCR. Results show thatHPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and severalHPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPVDNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective,suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support theproposed role of HPV in decreased fertility and prompt new possible consequences of theinfection in semen.

AB - Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseasescausing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPVinfection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developeda new approach to evaluate virus localisation in the different semen components. We analysedalso the specific genotype localisation and viral DNA quantity by qPCR. Results show thatHPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and severalHPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPVDNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective,suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support theproposed role of HPV in decreased fertility and prompt new possible consequences of theinfection in semen.

KW - HPV infection

KW - male infertility

KW - semen infection

KW - semen parameters

KW - sexually transmitted diseases

KW - viral infection

UR - http://hdl.handle.net/10447/358229

M3 - Article

VL - 147

SP - 1

EP - 8

JO - Epidemiology and Infection

JF - Epidemiology and Infection

SN - 0950-2688

ER -