Human papillomavirus (HPV) is the agent of the most common sexually transmitted diseasescausing a variety of clinical manifestations ranging from warts to cancer. Oncogenic HPVinfection is the major cause of cervical cancer and less frequently of penile cancers. Its presence in semen is widely known, but the effects on fertility are still controversial. We developeda new approach to evaluate virus localisation in the different semen components. We analysedalso the specific genotype localisation and viral DNA quantity by qPCR. Results show thatHPV DNA can be identified in every fraction of semen: spermatozoa, somatic cells and seminal plasma. Different samples can contain the HPV DNA in different fractions and severalHPV genotypes can be found in the same fraction. Additionally, different fractions may contain multiple HPV genotypes in different relative quantity. We analysed the wholeness of HPVDNA in sperm cells by qPCR. In one sample more than half of viral genomes were defective,suggesting a possible recombination event. The new method allows to easily distinguish different sperm infections and to observe the possible effects on semen. The data support theproposed role of HPV in decreased fertility and prompt new possible consequences of theinfection in semen.
|Numero di pagine||8|
|Rivista||Epidemiology and Infection|
|Stato di pubblicazione||Published - 2019|
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