TY - JOUR
T1 - HPV infection in relation to OSCC histological grading and TNM stage. Evalutation by traditional statistics and fuzzy logic model
AU - D'Angelo, Matteo
AU - Matranga, Domenica
AU - Campisi, Giuseppina
AU - Capra, Giuseppina
AU - Colella, Giuseppe
AU - Lo Muzio, Lorenzo
AU - Lo Muzio, L. Lo
AU - Leao, null
AU - Matranga, Domenica
AU - Capogreco, null
AU - Di Liberto, Chiara
AU - Calvino, Francesco Maria
PY - 2006
Y1 - 2006
N2 - We aimed to evaluate if in oral squamous cell carcinoma (OSCC) there is a relationshipbetween histological grading (HG), TNM clinical stage and HPV infection; and to study theperformance of fuzzy logic compared to traditional statistics, in the analysis of HPV status andcorrelates of OSCC. In cross-sectional analysis, the study group comprised 63 patients (meanage 68.89 years (SD ± 11.78), range (32–93); males 28 (44.4%), females 35 (55.6%)) with OSCChistologically diagnosed. HPV-DNA was studied in exfoliated oral epithelial cells by nested PCR(MY09/MY11 and GP5+/GP6+ primers). Data were analysed in parallel by traditional statisticswith multivariate analysis and a fuzzy logic (FL) technique (membership functions as input,the ANFIS methodology, and the Sugeno’s model of first order). HPV infection was detectedin 24/63 (38.1%) of OSCC, as being HPV+ve 14/36 (38.9%) in G1, 7/18 (38.9%) in G2, and 3/9(33.3%) in G3; HPV+ve 8/33 (24.2%) in Stage I, 9/12 (75.0%) in Stage II, 6/11(54.5%) in StageIII, and 1/7 (14.3%) in Stage IV. In both methods of analysis, no significantly increased risk ofHPV infection was found for any HG score; whereas, TNM stage II was significantly associatedto HPV infection (p = 0.004; OR = 9.375 (95% CI = 2.030:43.30); OR0 = 11.148 (95%CI = 1.951:43.30)), and, in particular, to primary tumour size T2 (p = 0.0036; OR = 7.812 (95% CI = 1.914:31.890); OR0 = 9.414 (95% CI = 1.846:48.013)); FL (% of prevision: 79.8; Root MeanSquareError (RMSE): 0.29). No association was found between HPV infection and anydemographical variable. Our findings show an association between HPV infection with TNM(stage II – T2), but not with histological grading of OSCC. Also, FL seems to be an additionaleffective tool in analysing the relationship of HPV infection with correlates of OSCC.c 2005 Elsevier Ltd. All rights reserved.
AB - We aimed to evaluate if in oral squamous cell carcinoma (OSCC) there is a relationshipbetween histological grading (HG), TNM clinical stage and HPV infection; and to study theperformance of fuzzy logic compared to traditional statistics, in the analysis of HPV status andcorrelates of OSCC. In cross-sectional analysis, the study group comprised 63 patients (meanage 68.89 years (SD ± 11.78), range (32–93); males 28 (44.4%), females 35 (55.6%)) with OSCChistologically diagnosed. HPV-DNA was studied in exfoliated oral epithelial cells by nested PCR(MY09/MY11 and GP5+/GP6+ primers). Data were analysed in parallel by traditional statisticswith multivariate analysis and a fuzzy logic (FL) technique (membership functions as input,the ANFIS methodology, and the Sugeno’s model of first order). HPV infection was detectedin 24/63 (38.1%) of OSCC, as being HPV+ve 14/36 (38.9%) in G1, 7/18 (38.9%) in G2, and 3/9(33.3%) in G3; HPV+ve 8/33 (24.2%) in Stage I, 9/12 (75.0%) in Stage II, 6/11(54.5%) in StageIII, and 1/7 (14.3%) in Stage IV. In both methods of analysis, no significantly increased risk ofHPV infection was found for any HG score; whereas, TNM stage II was significantly associatedto HPV infection (p = 0.004; OR = 9.375 (95% CI = 2.030:43.30); OR0 = 11.148 (95%CI = 1.951:43.30)), and, in particular, to primary tumour size T2 (p = 0.0036; OR = 7.812 (95% CI = 1.914:31.890); OR0 = 9.414 (95% CI = 1.846:48.013)); FL (% of prevision: 79.8; Root MeanSquareError (RMSE): 0.29). No association was found between HPV infection and anydemographical variable. Our findings show an association between HPV infection with TNM(stage II – T2), but not with histological grading of OSCC. Also, FL seems to be an additionaleffective tool in analysing the relationship of HPV infection with correlates of OSCC.c 2005 Elsevier Ltd. All rights reserved.
UR - http://hdl.handle.net/10447/34220
M3 - Article
VL - 42
SP - 638
EP - 645
JO - Oral Oncology
JF - Oral Oncology
SN - 1368-8375
ER -