High genetic stability of potato yellow mosaic Panama virus infecting tomato in Panama

Stefano Panno, Andrea Giovanni Caruso, Salvatore Davino, Salvatore Davino, Mario Davino, José Angel Herrera Vásquez

Risultato della ricerca: Article

Abstract

The relevant regions in Panama involved in commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santosand Panama Oeste provinces, were surveyed for the distribution and genetic diversity of potato yellow mosaic Panama virus(PYMPV) in the growing seasons of 2011 and 2012. A total of 28 tomato plots were surveyed and 314 individual tomato plantswere sampled. DNA was extracted from each plant for a subsequent rolling circle amplification (RCA) analysis, to confirm thepresence of begomovirus infections. The samples displaying a positive RCA reaction were subsequently analysed by PCR with aspecific primer pair to identify PYMPV. This virus was detected in samples collected in all the above-mentioned provinces. Thepopulation structure of PYMPV was assessed by single-strand conformation polymorphism (SSCP) analysis. In total, four distinctSSCP patterns were distinguished. Based on the geographical distribution and the different patterns obtained, 42 viral isolates wereselected for cloning and sequencing. Sequences obtained were compared with the ancestral sequence of PYMPV reported inPanama in 1998. These sequences showed very low variability, with two lineages probably originated by a single introduction.
Lingua originaleEnglish
pagine (da-a)59-65
Numero di pagine7
RivistaJournal of Plant Pathology
Volume100
Stato di pubblicazionePublished - 2018

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genetic stability
Panama
tomatoes
Begomovirus
single-stranded conformational polymorphism
molecular cloning
geographical distribution
growing season
sampling
viruses
genetic variation
Potato yellow mosaic Panama virus
DNA
infection

All Science Journal Classification (ASJC) codes

  • Plant Science

Cita questo

High genetic stability of potato yellow mosaic Panama virus infecting tomato in Panama. / Panno, Stefano; Caruso, Andrea Giovanni; Davino, Salvatore; Davino, Salvatore; Davino, Mario; Herrera Vásquez, José Angel.

In: Journal of Plant Pathology, Vol. 100, 2018, pag. 59-65.

Risultato della ricerca: Article

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AU - Caruso, Andrea Giovanni

AU - Davino, Salvatore

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AU - Davino, Mario

AU - Herrera Vásquez, José Angel

PY - 2018

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AB - The relevant regions in Panama involved in commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santosand Panama Oeste provinces, were surveyed for the distribution and genetic diversity of potato yellow mosaic Panama virus(PYMPV) in the growing seasons of 2011 and 2012. A total of 28 tomato plots were surveyed and 314 individual tomato plantswere sampled. DNA was extracted from each plant for a subsequent rolling circle amplification (RCA) analysis, to confirm thepresence of begomovirus infections. The samples displaying a positive RCA reaction were subsequently analysed by PCR with aspecific primer pair to identify PYMPV. This virus was detected in samples collected in all the above-mentioned provinces. Thepopulation structure of PYMPV was assessed by single-strand conformation polymorphism (SSCP) analysis. In total, four distinctSSCP patterns were distinguished. Based on the geographical distribution and the different patterns obtained, 42 viral isolates wereselected for cloning and sequencing. Sequences obtained were compared with the ancestral sequence of PYMPV reported inPanama in 1998. These sequences showed very low variability, with two lineages probably originated by a single introduction.

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