High energy radiation processing for the synthesis of insulin nanocarriers for the development of a new strategy for the treatment of Alzheimer's Disease

Risultato della ricerca: Other


Nanogels are nanoscalar polymeric networks, characterized by a dynamic internal structure and a flexibleadaptive shape. When they are used as drug carriers, their flexibility and shape changing ability can facilitatethe bypass of biological barriers, ensure protection of the payload and enable interaction of any attachedligand with its receptors. Poly(N-vinyl pyrrolidone)-co-acrylic acid nanogels (NGs) have been produced by ebeamirradiation of diluted aqueous solutions of a water-soluble polymer in the presence of a small amountof acrylic acid. In particular, industrial accelerators and the typical set-ups and doses applied for sterilizationhave been used. [1, 2] Particle size, molecular weight and functionalization can be controlled by a properselection of polymer concentration and irradiation parameters, such as dose-rate and total dose imparted [3].In recent years, a growing body of evidence has linked insulin resistance and insulin action to Alzheimer’sDisease (AD), a condition also referred to as Type 3 Diabetes (T3D) [4,5]. Recently, it has beendemonstrated that insulin is capable of reducing toxicity induced by A oligomers, the protein mainly involvedin AD onset, by inhibition of the intrinsic apoptotic pathway [6]. Moreover, activation of insulin signalingprovides a neuroprotective mechanism to counteract oxidative stress, mitochondrial damage andneurodegeneration triggered by A oligomers in neuroblastoma cells [7]. In the present work NGs have beenconjugated to insulin (NG-In) to defend the hormone from enzymatic degradation, facilitate its crossingthrough a blood brain barrier model and protect the LAN5 cells from the damages induced by A oligomersaddiction.
Lingua originaleEnglish
Numero di pagine2
Stato di pubblicazionePublished - 2016


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