TY - JOUR
T1 - High-density ZnO Nanowires as a Reversible Myogenic-Differentiation-Switch
AU - Arrabito, Giuseppe Domenico
AU - Errico, Vito
AU - Fuoco, Claudia
AU - Desideri, Alessandro
AU - Testa, Stefano
AU - Arrabito, Giuseppe
AU - Fornetti, Ersilia
AU - Rufini, Stefano
AU - Gargioli, Cesare
AU - Saggio, Giovanni
AU - Cannata, Stefano
AU - Falconi, Christian
PY - 2018
Y1 - 2018
N2 - Mesoangioblasts are outstanding candidates for stem cell therapy and are already being explored in clinical trials. However, a crucial challenge in regenerative medicine is the limited availability of undifferentiated myogenic progenitor cells, since growth is typically accompanied by differentiation. Here reversible myogenic-differentiation-switching during proliferation is achieved by functionalizing the glass substrate with high-density ZnO nanowires. Specifically, mesoangioblasts grown on ZnO nanowires present a spherical viable undifferentiated cell state without lamellopodia formation during all the observation time (8 days). Consistently, the Myosin Heavy Chain, typically expressed in skeletal muscle tissue and differentiated myogenic progenitors, is completely absent. Remarkably, nanowires do not induce any damage while reversibly block differentiation, so that the differentiation capabilities are completely recovered upon cells removal from the nanowires-functionalized substrate and re-plating on standard culture glass. This is the first evidence of a reversible myogenic-differentiation switch which does not affect viability. These results can be the first step toward the in vitro growth of a large number of undifferentiated stem/progenitor cells and therefore can represent a breakthrough for cell based therapy and tissue engineering.
AB - Mesoangioblasts are outstanding candidates for stem cell therapy and are already being explored in clinical trials. However, a crucial challenge in regenerative medicine is the limited availability of undifferentiated myogenic progenitor cells, since growth is typically accompanied by differentiation. Here reversible myogenic-differentiation-switching during proliferation is achieved by functionalizing the glass substrate with high-density ZnO nanowires. Specifically, mesoangioblasts grown on ZnO nanowires present a spherical viable undifferentiated cell state without lamellopodia formation during all the observation time (8 days). Consistently, the Myosin Heavy Chain, typically expressed in skeletal muscle tissue and differentiated myogenic progenitors, is completely absent. Remarkably, nanowires do not induce any damage while reversibly block differentiation, so that the differentiation capabilities are completely recovered upon cells removal from the nanowires-functionalized substrate and re-plating on standard culture glass. This is the first evidence of a reversible myogenic-differentiation switch which does not affect viability. These results can be the first step toward the in vitro growth of a large number of undifferentiated stem/progenitor cells and therefore can represent a breakthrough for cell based therapy and tissue engineering.
KW - ZnO nanowires
KW - mesoangioblasts
KW - muscle differentiation
KW - tissue engineering
KW - ZnO nanowires
KW - mesoangioblasts
KW - muscle differentiation
KW - tissue engineering
UR - http://hdl.handle.net/10447/287134
UR - https://pubs.acs.org/doi/10.1021/acsami.7b19758
M3 - Article
VL - 10
SP - 14097
EP - 14107
JO - ACS APPLIED MATERIALS & INTERFACES
JF - ACS APPLIED MATERIALS & INTERFACES
SN - 1944-8244
ER -