Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 57-64 |
| Numero di pagine | 8 |
| Rivista | European Journal of Pharmaceutical Sciences |
| Volume | 43 |
| Stato di pubblicazione | Published - 2011 |
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All Science Journal Classification (ASJC) codes
- Pharmaceutical Science
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Hemoglobin loaded polymeric nanoparticles: preparation and characterizations. / Schiro', Giorgio; Levantino, Matteo; Cupane, Antonio; Dessy, Alberto; Chiellini, Federica; Piras, Anna M.
In: European Journal of Pharmaceutical Sciences, Vol. 43, 2011, pag. 57-64.Risultato della ricerca: Article
}
TY - JOUR
T1 - Hemoglobin loaded polymeric nanoparticles: preparation and characterizations
AU - Schiro', Giorgio
AU - Levantino, Matteo
AU - Cupane, Antonio
AU - Dessy, Alberto
AU - Chiellini, Federica
AU - Piras, Anna M.
PY - 2011
Y1 - 2011
N2 - In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.
AB - In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.
KW - Biocompatible polymers
KW - Blood substitutes
KW - Injectable systems
KW - Polymeric nanoparticles
UR - http://hdl.handle.net/10447/53895
UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T25-52G8GKN-2&_user=519924&_coverDate=05%2F18%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000025965&_version=1&_urlVersion=0&_userid=519924&md5=728165cf5a146eba4d1ee81dd52dac9e&searchtype=a
M3 - Article
VL - 43
SP - 57
EP - 64
JO - European Journal of Pharmaceutical Sciences
JF - European Journal of Pharmaceutical Sciences
SN - 0928-0987
ER -