Hemoglobin loaded polymeric nanoparticles: preparation and characterizations

Antonio Cupane, Matteo Levantino, Giorgio Schiro', Federica Chiellini, Anna M. Piras, Alberto Dessy

Risultato della ricerca: Article

14 Citazioni (Scopus)

Abstract

In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.
Lingua originaleEnglish
pagine (da-a)57-64
Numero di pagine8
RivistaEuropean Journal of Pharmaceutical Sciences
Volume43
Stato di pubblicazionePublished - 2011

Fingerprint

Nanoparticles
Hemoglobins
Maleic Anhydrides
Reducing Agents
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cita questo

Hemoglobin loaded polymeric nanoparticles: preparation and characterizations. / Cupane, Antonio; Levantino, Matteo; Schiro', Giorgio; Chiellini, Federica; Piras, Anna M.; Dessy, Alberto.

In: European Journal of Pharmaceutical Sciences, Vol. 43, 2011, pag. 57-64.

Risultato della ricerca: Article

@article{90717c02afa343fea645c7b132979e64,
title = "Hemoglobin loaded polymeric nanoparticles: preparation and characterizations",
abstract = "In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5{\%} grafted with m-PEG (2000) and 95{\%} grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.",
keywords = "Polymeric nanoparticles; Blood substitutes; Biocompatible polymers; Injectable systems",
author = "Antonio Cupane and Matteo Levantino and Giorgio Schiro' and Federica Chiellini and Piras, {Anna M.} and Alberto Dessy",
year = "2011",
language = "English",
volume = "43",
pages = "57--64",
journal = "European Journal of Pharmaceutical Sciences",
issn = "0928-0987",
publisher = "Elsevier",

}

TY - JOUR

T1 - Hemoglobin loaded polymeric nanoparticles: preparation and characterizations

AU - Cupane, Antonio

AU - Levantino, Matteo

AU - Schiro', Giorgio

AU - Chiellini, Federica

AU - Piras, Anna M.

AU - Dessy, Alberto

PY - 2011

Y1 - 2011

N2 - In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.

AB - In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138 ± 10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.

KW - Polymeric nanoparticles; Blood substitutes; Biocompatible polymers; Injectable systems

UR - http://hdl.handle.net/10447/53895

UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T25-52G8GKN-2&_user=519924&_coverDate=05%2F18%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000025965&_version=1&_urlVersion=0&_userid=519924&md5=728165cf5a146eba4d1ee81dd52dac9e&searchtype=a

M3 - Article

VL - 43

SP - 57

EP - 64

JO - European Journal of Pharmaceutical Sciences

JF - European Journal of Pharmaceutical Sciences

SN - 0928-0987

ER -