Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery

Francesco Cappello, Giovanni Zummo, Alessandro Pitruzzella, Girolamo Geraci, Rosario Barone, Antonella Marino Gammazza, Francesca Rappa, Carmelo Sciume', Felicia Farina, Giuseppe Modica, Celeste Caruso Bavisotto, Sabrina David, Claudia Campanella, Fabio Bucchieri, Giuseppe Curcuru', Claudia Campanella, Antonella Marino Gammazza, Alessandro Pitruzzella, Rosario Barone, Francesca RappaCeleste Caruso Bavisotto, Giuseppe Curcurù, Fabio Bucchieri, Stefano Fais, Francesco Cappello, Everly Conway De Macario, Alberto J.L. Macario

Risultato della ricerca: Article

47 Citazioni (Scopus)

Abstract

BACKGROUND: Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer. METHODS: The authors studied a population of 97 subjects, including patients and controls. Immunomorphology, Western blot analysis, and quantitative real-time polymerase chain reaction were performed on tissue specimens. Exosomes were isolated from blood and characterized by electron microscopy, biochemical tests, and Western blot analysis. RESULTS: Hsp60 was found to be increased in cancerous tissue, in which it was localized in the tumor cell plasma membrane, and in the interstitium associated with cells of the immune system, in which it was associated with exosomes liberated by tumor cells and, as such, circulated in the blood. An interesting finding was that these parameters returned to normal shortly after tumor removal. CONCLUSIONS: The data from the current study suggested that Hsp60 is a good candidate for theranostics applied to patients with large bowel carcinoma and encourage similar research among patients with other tumors in which Hsp60 has been implicated.
Lingua originaleEnglish
pagine (da-a)3230-9-3239
Numero di pagine10
RivistaCancer
Volume121
Stato di pubblicazionePublished - 2015

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Exosomes
Chaperonin 60
Colonic Neoplasms
Neoplasms
Western Blotting
Cell Membrane
Chaperonins
Tumor Biomarkers
Real-Time Polymerase Chain Reaction
Immune System
Electron Microscopy
Carcinogenesis
Biomarkers
Carcinoma
Research
Population

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cita questo

Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery. / Cappello, Francesco; Zummo, Giovanni; Pitruzzella, Alessandro; Geraci, Girolamo; Barone, Rosario; Marino Gammazza, Antonella; Rappa, Francesca; Sciume', Carmelo; Farina, Felicia; Modica, Giuseppe; Caruso Bavisotto, Celeste; David, Sabrina; Campanella, Claudia; Bucchieri, Fabio; Curcuru', Giuseppe; Campanella, Claudia; Marino Gammazza, Antonella; Pitruzzella, Alessandro; Barone, Rosario; Rappa, Francesca; Caruso Bavisotto, Celeste; Curcurù, Giuseppe; Bucchieri, Fabio; Fais, Stefano; Cappello, Francesco; Conway De Macario, Everly; Macario, Alberto J.L.

In: Cancer, Vol. 121, 2015, pag. 3230-9-3239.

Risultato della ricerca: Article

Cappello, F, Zummo, G, Pitruzzella, A, Geraci, G, Barone, R, Marino Gammazza, A, Rappa, F, Sciume', C, Farina, F, Modica, G, Caruso Bavisotto, C, David, S, Campanella, C, Bucchieri, F, Curcuru', G, Campanella, C, Marino Gammazza, A, Pitruzzella, A, Barone, R, Rappa, F, Caruso Bavisotto, C, Curcurù, G, Bucchieri, F, Fais, S, Cappello, F, Conway De Macario, E & Macario, AJL 2015, 'Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery', Cancer, vol. 121, pagg. 3230-9-3239.
Cappello, Francesco ; Zummo, Giovanni ; Pitruzzella, Alessandro ; Geraci, Girolamo ; Barone, Rosario ; Marino Gammazza, Antonella ; Rappa, Francesca ; Sciume', Carmelo ; Farina, Felicia ; Modica, Giuseppe ; Caruso Bavisotto, Celeste ; David, Sabrina ; Campanella, Claudia ; Bucchieri, Fabio ; Curcuru', Giuseppe ; Campanella, Claudia ; Marino Gammazza, Antonella ; Pitruzzella, Alessandro ; Barone, Rosario ; Rappa, Francesca ; Caruso Bavisotto, Celeste ; Curcurù, Giuseppe ; Bucchieri, Fabio ; Fais, Stefano ; Cappello, Francesco ; Conway De Macario, Everly ; Macario, Alberto J.L. / Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery. In: Cancer. 2015 ; Vol. 121. pagg. 3230-9-3239.
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title = "Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery",
abstract = "BACKGROUND: Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer. METHODS: The authors studied a population of 97 subjects, including patients and controls. Immunomorphology, Western blot analysis, and quantitative real-time polymerase chain reaction were performed on tissue specimens. Exosomes were isolated from blood and characterized by electron microscopy, biochemical tests, and Western blot analysis. RESULTS: Hsp60 was found to be increased in cancerous tissue, in which it was localized in the tumor cell plasma membrane, and in the interstitium associated with cells of the immune system, in which it was associated with exosomes liberated by tumor cells and, as such, circulated in the blood. An interesting finding was that these parameters returned to normal shortly after tumor removal. CONCLUSIONS: The data from the current study suggested that Hsp60 is a good candidate for theranostics applied to patients with large bowel carcinoma and encourage similar research among patients with other tumors in which Hsp60 has been implicated.",
author = "Francesco Cappello and Giovanni Zummo and Alessandro Pitruzzella and Girolamo Geraci and Rosario Barone and {Marino Gammazza}, Antonella and Francesca Rappa and Carmelo Sciume' and Felicia Farina and Giuseppe Modica and {Caruso Bavisotto}, Celeste and Sabrina David and Claudia Campanella and Fabio Bucchieri and Giuseppe Curcuru' and Claudia Campanella and {Marino Gammazza}, Antonella and Alessandro Pitruzzella and Rosario Barone and Francesca Rappa and {Caruso Bavisotto}, Celeste and Giuseppe Curcur{\`u} and Fabio Bucchieri and Stefano Fais and Francesco Cappello and {Conway De Macario}, Everly and Macario, {Alberto J.L.}",
year = "2015",
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TY - JOUR

T1 - Heat shock protein 60 levels in tissue and circulating exosomes in human large bowel cancer before and after ablative surgery

AU - Cappello, Francesco

AU - Zummo, Giovanni

AU - Pitruzzella, Alessandro

AU - Geraci, Girolamo

AU - Barone, Rosario

AU - Marino Gammazza, Antonella

AU - Rappa, Francesca

AU - Sciume', Carmelo

AU - Farina, Felicia

AU - Modica, Giuseppe

AU - Caruso Bavisotto, Celeste

AU - David, Sabrina

AU - Campanella, Claudia

AU - Bucchieri, Fabio

AU - Curcuru', Giuseppe

AU - Campanella, Claudia

AU - Marino Gammazza, Antonella

AU - Pitruzzella, Alessandro

AU - Barone, Rosario

AU - Rappa, Francesca

AU - Caruso Bavisotto, Celeste

AU - Curcurù, Giuseppe

AU - Bucchieri, Fabio

AU - Fais, Stefano

AU - Cappello, Francesco

AU - Conway De Macario, Everly

AU - Macario, Alberto J.L.

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer. METHODS: The authors studied a population of 97 subjects, including patients and controls. Immunomorphology, Western blot analysis, and quantitative real-time polymerase chain reaction were performed on tissue specimens. Exosomes were isolated from blood and characterized by electron microscopy, biochemical tests, and Western blot analysis. RESULTS: Hsp60 was found to be increased in cancerous tissue, in which it was localized in the tumor cell plasma membrane, and in the interstitium associated with cells of the immune system, in which it was associated with exosomes liberated by tumor cells and, as such, circulated in the blood. An interesting finding was that these parameters returned to normal shortly after tumor removal. CONCLUSIONS: The data from the current study suggested that Hsp60 is a good candidate for theranostics applied to patients with large bowel carcinoma and encourage similar research among patients with other tumors in which Hsp60 has been implicated.

AB - BACKGROUND: Heat shock protein 60 (Hsp60) is a chaperonin involved in tumorigenesis, but its participation in tumor development and progression is not well understood and its value as a tumor biomarker has not been fully elucidated. In the current study, the authors presented evidence supporting the theory that Hsp60 has potential as a biomarker as well as a therapeutic target in patients with large bowel cancer. METHODS: The authors studied a population of 97 subjects, including patients and controls. Immunomorphology, Western blot analysis, and quantitative real-time polymerase chain reaction were performed on tissue specimens. Exosomes were isolated from blood and characterized by electron microscopy, biochemical tests, and Western blot analysis. RESULTS: Hsp60 was found to be increased in cancerous tissue, in which it was localized in the tumor cell plasma membrane, and in the interstitium associated with cells of the immune system, in which it was associated with exosomes liberated by tumor cells and, as such, circulated in the blood. An interesting finding was that these parameters returned to normal shortly after tumor removal. CONCLUSIONS: The data from the current study suggested that Hsp60 is a good candidate for theranostics applied to patients with large bowel carcinoma and encourage similar research among patients with other tumors in which Hsp60 has been implicated.

UR - http://hdl.handle.net/10447/145501

UR - http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0142

M3 - Article

VL - 121

SP - 3230-9-3239

JO - Cancer

JF - Cancer

SN - 0008-543X

ER -