Halloysite nanotubes-carbon dots hybrids multifunctional nanocarrier with positive cell target ability as a potential non-viral vector for oral gene therapy

Giuseppe Lazzara, Giuseppa Biddeci, Giuseppe Cavallaro, Marina Massaro, Giampaolo Barone, Serena Riela, Gaetano Spinelli, Giuseppa Biddeci, Francesco Di Blasi, Giuseppe Nicotra, Corrado Spinella, Francesco Di Blasi

Risultato della ricerca: Article

5 Citazioni (Scopus)

Abstract

Hypothesis: The use of non-viral vectors for gene therapy is hindered by their lower transfection effi- ciency and their lacking of self-track ability.Experiments: This study aims to investigate the biological properties of halloysite nanotubes-carbon dots hybrid and its potential use as non-viral vector for oral gene therapy. The morphology and the chemical composition of the halloysite hybrid were investigated by means of high angle annular dark field scan- ning TEM and electron energy loss spectroscopy techniques, respectively. The cytotoxicity and the antioxidant activity were investigated by standard methods (MTS, DPPH and H2O2, respectively) using human cervical cancer HeLa cells as model. Studies of cellular uptake were carried out by fluorescence microscopy. Finally, we investigated the loading and release ability of the hybrid versus calf thymus DNA by fluorescence microscopy, circular dichroism, dynamic light scattering and f-potential measure- ments.Findings: All investigations performed confirmed the existence of strong electrostatic interactions between the DNA and the halloysite hybrid, so it shows promise as a multi-functional cationic non- viral vector that has also possesses intracellular tracking capability and promising in vitro antioxidant potential.
Lingua originaleEnglish
pagine (da-a)236-246
Numero di pagine11
RivistaJournal of Colloid and Interface Science
Volume552
Stato di pubblicazionePublished - 2019

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Gene therapy
Carbon Nanotubes
Carbon nanotubes
Fluorescence microscopy
Cells
Antioxidants
DNA
Thymus
Electron energy loss spectroscopy
Dichroism
Dynamic light scattering
Cytotoxicity
Coulomb interactions
Transmission electron microscopy
Scanning
Chemical analysis
clay
Experiments

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Cita questo

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title = "Halloysite nanotubes-carbon dots hybrids multifunctional nanocarrier with positive cell target ability as a potential non-viral vector for oral gene therapy",
abstract = "Hypothesis: The use of non-viral vectors for gene therapy is hindered by their lower transfection effi- ciency and their lacking of self-track ability.Experiments: This study aims to investigate the biological properties of halloysite nanotubes-carbon dots hybrid and its potential use as non-viral vector for oral gene therapy. The morphology and the chemical composition of the halloysite hybrid were investigated by means of high angle annular dark field scan- ning TEM and electron energy loss spectroscopy techniques, respectively. The cytotoxicity and the antioxidant activity were investigated by standard methods (MTS, DPPH and H2O2, respectively) using human cervical cancer HeLa cells as model. Studies of cellular uptake were carried out by fluorescence microscopy. Finally, we investigated the loading and release ability of the hybrid versus calf thymus DNA by fluorescence microscopy, circular dichroism, dynamic light scattering and f-potential measure- ments.Findings: All investigations performed confirmed the existence of strong electrostatic interactions between the DNA and the halloysite hybrid, so it shows promise as a multi-functional cationic non- viral vector that has also possesses intracellular tracking capability and promising in vitro antioxidant potential.",
author = "Giuseppe Lazzara and Giuseppa Biddeci and Giuseppe Cavallaro and Marina Massaro and Giampaolo Barone and Serena Riela and Gaetano Spinelli and Giuseppa Biddeci and {Di Blasi}, Francesco and Giuseppe Nicotra and Corrado Spinella and {Di Blasi}, Francesco",
year = "2019",
language = "English",
volume = "552",
pages = "236--246",
journal = "Journal of Colloid and Interface Science",
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TY - JOUR

T1 - Halloysite nanotubes-carbon dots hybrids multifunctional nanocarrier with positive cell target ability as a potential non-viral vector for oral gene therapy

AU - Lazzara, Giuseppe

AU - Biddeci, Giuseppa

AU - Cavallaro, Giuseppe

AU - Massaro, Marina

AU - Barone, Giampaolo

AU - Riela, Serena

AU - Spinelli, Gaetano

AU - Biddeci, Giuseppa

AU - Di Blasi, Francesco

AU - Nicotra, Giuseppe

AU - Spinella, Corrado

AU - Di Blasi, Francesco

PY - 2019

Y1 - 2019

N2 - Hypothesis: The use of non-viral vectors for gene therapy is hindered by their lower transfection effi- ciency and their lacking of self-track ability.Experiments: This study aims to investigate the biological properties of halloysite nanotubes-carbon dots hybrid and its potential use as non-viral vector for oral gene therapy. The morphology and the chemical composition of the halloysite hybrid were investigated by means of high angle annular dark field scan- ning TEM and electron energy loss spectroscopy techniques, respectively. The cytotoxicity and the antioxidant activity were investigated by standard methods (MTS, DPPH and H2O2, respectively) using human cervical cancer HeLa cells as model. Studies of cellular uptake were carried out by fluorescence microscopy. Finally, we investigated the loading and release ability of the hybrid versus calf thymus DNA by fluorescence microscopy, circular dichroism, dynamic light scattering and f-potential measure- ments.Findings: All investigations performed confirmed the existence of strong electrostatic interactions between the DNA and the halloysite hybrid, so it shows promise as a multi-functional cationic non- viral vector that has also possesses intracellular tracking capability and promising in vitro antioxidant potential.

AB - Hypothesis: The use of non-viral vectors for gene therapy is hindered by their lower transfection effi- ciency and their lacking of self-track ability.Experiments: This study aims to investigate the biological properties of halloysite nanotubes-carbon dots hybrid and its potential use as non-viral vector for oral gene therapy. The morphology and the chemical composition of the halloysite hybrid were investigated by means of high angle annular dark field scan- ning TEM and electron energy loss spectroscopy techniques, respectively. The cytotoxicity and the antioxidant activity were investigated by standard methods (MTS, DPPH and H2O2, respectively) using human cervical cancer HeLa cells as model. Studies of cellular uptake were carried out by fluorescence microscopy. Finally, we investigated the loading and release ability of the hybrid versus calf thymus DNA by fluorescence microscopy, circular dichroism, dynamic light scattering and f-potential measure- ments.Findings: All investigations performed confirmed the existence of strong electrostatic interactions between the DNA and the halloysite hybrid, so it shows promise as a multi-functional cationic non- viral vector that has also possesses intracellular tracking capability and promising in vitro antioxidant potential.

UR - http://hdl.handle.net/10447/368359

M3 - Article

VL - 552

SP - 236

EP - 246

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -