Guanine-based purines affects the enteric cholinergic neurotransmission via amechanism not involving membrane receptors

Risultato della ricerca: Other

Abstract

Increasing evidence indicate that guanine-basedpurines, known as modulators of intracellularprocesses, can exert extracellular effects, raisingthe possibility of the existence of specific receptorsfor these compounds. We investigated if guaninebasedpurine receptors may be present in the rodentgastrointestinal tract modulating intestinalcontractility, as the well known adenine-basedpurine receptors. Experiments were performed invitro recording spontaneous and neurally-evokedcontractile activity, as changes in isometric tension,in mouse distal colon circular muscle.Guanosine up to 3 mM or guanine up to 1 mM, didnot affect the spontaneous mechanical activity, butthey significantly and reversibly reduced theamplitude of the nerve evoked cholinergiccontractions. Both compounds did not affect thedirect contractile responses to muscarinic agonist.No desensitization of the response was observed.Guanine-based purine effects were not modified byadenine-based purine receptor antagonists or byadenylyl or guanilyl cyclase inhibitors.Dipyridamole or NBTI, nucleoside uptakeinhibitors markedly reduced the guanosine effectswhilst guanine effects were prevented in thepresence of adenine, competitive inhibitor ofnucleobase uptake.Our data indicate that guanosine and guanine areable to modulate negatively the excitatorycholinergic neurotransmission in the mouse coloncircular muscle. Guanine-based purines appear toact on prejunctional release of acetylcholine. Theireffects are dependent by their cellular uptake, andindependent by adenine based purine receptors.
Lingua originaleEnglish
Numero di pagine1
Stato di pubblicazionePublished - 2011

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Purines
Guanine
Synaptic Transmission
Cholinergic Agents
Membranes
Guanosine
Adenine
Purinergic Receptors
Muscarinic Agonists
Muscles
Dipyridamole
Nucleosides
Acetylcholine
Colon

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title = "Guanine-based purines affects the enteric cholinergic neurotransmission via amechanism not involving membrane receptors",
abstract = "Increasing evidence indicate that guanine-basedpurines, known as modulators of intracellularprocesses, can exert extracellular effects, raisingthe possibility of the existence of specific receptorsfor these compounds. We investigated if guaninebasedpurine receptors may be present in the rodentgastrointestinal tract modulating intestinalcontractility, as the well known adenine-basedpurine receptors. Experiments were performed invitro recording spontaneous and neurally-evokedcontractile activity, as changes in isometric tension,in mouse distal colon circular muscle.Guanosine up to 3 mM or guanine up to 1 mM, didnot affect the spontaneous mechanical activity, butthey significantly and reversibly reduced theamplitude of the nerve evoked cholinergiccontractions. Both compounds did not affect thedirect contractile responses to muscarinic agonist.No desensitization of the response was observed.Guanine-based purine effects were not modified byadenine-based purine receptor antagonists or byadenylyl or guanilyl cyclase inhibitors.Dipyridamole or NBTI, nucleoside uptakeinhibitors markedly reduced the guanosine effectswhilst guanine effects were prevented in thepresence of adenine, competitive inhibitor ofnucleobase uptake.Our data indicate that guanosine and guanine areable to modulate negatively the excitatorycholinergic neurotransmission in the mouse coloncircular muscle. Guanine-based purines appear toact on prejunctional release of acetylcholine. Theireffects are dependent by their cellular uptake, andindependent by adenine based purine receptors.",
keywords = "enteric transmission, guanine based purine, mouse colon",
author = "Flavia Mule' and Serio, {Rosa Maria} and Mariangela Mastropaolo and Natale Belluardo and Zizzo, {Maria Grazia}",
year = "2011",
language = "English",

}

TY - CONF

T1 - Guanine-based purines affects the enteric cholinergic neurotransmission via amechanism not involving membrane receptors

AU - Mule', Flavia

AU - Serio, Rosa Maria

AU - Mastropaolo, Mariangela

AU - Belluardo, Natale

AU - Zizzo, Maria Grazia

PY - 2011

Y1 - 2011

N2 - Increasing evidence indicate that guanine-basedpurines, known as modulators of intracellularprocesses, can exert extracellular effects, raisingthe possibility of the existence of specific receptorsfor these compounds. We investigated if guaninebasedpurine receptors may be present in the rodentgastrointestinal tract modulating intestinalcontractility, as the well known adenine-basedpurine receptors. Experiments were performed invitro recording spontaneous and neurally-evokedcontractile activity, as changes in isometric tension,in mouse distal colon circular muscle.Guanosine up to 3 mM or guanine up to 1 mM, didnot affect the spontaneous mechanical activity, butthey significantly and reversibly reduced theamplitude of the nerve evoked cholinergiccontractions. Both compounds did not affect thedirect contractile responses to muscarinic agonist.No desensitization of the response was observed.Guanine-based purine effects were not modified byadenine-based purine receptor antagonists or byadenylyl or guanilyl cyclase inhibitors.Dipyridamole or NBTI, nucleoside uptakeinhibitors markedly reduced the guanosine effectswhilst guanine effects were prevented in thepresence of adenine, competitive inhibitor ofnucleobase uptake.Our data indicate that guanosine and guanine areable to modulate negatively the excitatorycholinergic neurotransmission in the mouse coloncircular muscle. Guanine-based purines appear toact on prejunctional release of acetylcholine. Theireffects are dependent by their cellular uptake, andindependent by adenine based purine receptors.

AB - Increasing evidence indicate that guanine-basedpurines, known as modulators of intracellularprocesses, can exert extracellular effects, raisingthe possibility of the existence of specific receptorsfor these compounds. We investigated if guaninebasedpurine receptors may be present in the rodentgastrointestinal tract modulating intestinalcontractility, as the well known adenine-basedpurine receptors. Experiments were performed invitro recording spontaneous and neurally-evokedcontractile activity, as changes in isometric tension,in mouse distal colon circular muscle.Guanosine up to 3 mM or guanine up to 1 mM, didnot affect the spontaneous mechanical activity, butthey significantly and reversibly reduced theamplitude of the nerve evoked cholinergiccontractions. Both compounds did not affect thedirect contractile responses to muscarinic agonist.No desensitization of the response was observed.Guanine-based purine effects were not modified byadenine-based purine receptor antagonists or byadenylyl or guanilyl cyclase inhibitors.Dipyridamole or NBTI, nucleoside uptakeinhibitors markedly reduced the guanosine effectswhilst guanine effects were prevented in thepresence of adenine, competitive inhibitor ofnucleobase uptake.Our data indicate that guanosine and guanine areable to modulate negatively the excitatorycholinergic neurotransmission in the mouse coloncircular muscle. Guanine-based purines appear toact on prejunctional release of acetylcholine. Theireffects are dependent by their cellular uptake, andindependent by adenine based purine receptors.

KW - enteric transmission

KW - guanine based purine

KW - mouse colon

UR - http://hdl.handle.net/10447/53564

M3 - Other

ER -