Graphene nanosystems as supports in siRNA Delivery

Gaetano Giammona, Giulio Ghersi, Nicolò Mauro, Simona Campora, Simona Campora, Giulio Ghersi, Peter Griffiths

Risultato della ricerca: Article

1 Citazione (Scopus)

Abstract

The nature of graphene-based nanosystems, as well as the possibility to synthesize them at low cost, have made them in the last few years, an interesting proposition in tumour therapy as a drug delivery system. Here, a reduced form of graphene oxide (RGO) has been synthesized and conjugated with a specific antibody for active targeting against tumour cells (RGOY). Furthermore, its bi-dimensional nature permits also p- p stacking interactions with planar molecules like siRNA (RGOY-siRNA). All these nano-complexes were characterized by DLS and DSC analysis, TEM and Raman spectroscopy and their biocompatibility was demonstrated by viability assay on cell line ECV 304. The system is able to be internalized from cells, as demonstrated by uptake studies with confocal microscopy, performed using its red fluorescence variant (RGOY-AF). A high reduction of the stacking interactions between the graphene sheets was obtained by conjugating RGOY particles to polyvinylpyrrolidone (PVP) (RGOY-PVP). The addition of PVP did not alter the biocompatibility of the system, but limited the formation of aggregates due to the stacking interaction between the graphene sheets: the complexes appeared more dispersed and able to enter into the cells after only few minutes, and in higher amounts with respect to the complex without PVP. All obtained data indicate graphene nanosystems very good candidates as delivery system thanks to their specific properties that permit to link to both antibody and siRNA without any degradation effect.
Lingua originaleEnglish
pagine (da-a)415-420
Numero di pagine6
RivistaCHEMICAL ENGINEERING TRANSACTIONS
Volume64
Stato di pubblicazionePublished - 2018

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Nanosystems
Graphite
Povidone
Small Interfering RNA
Biocompatibility
Tumors
Cells
Antibodies
Confocal microscopy
Oxides
Raman spectroscopy
Assays
Fluorescence
Transmission electron microscopy
Degradation
Molecules
Costs

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)

Cita questo

Graphene nanosystems as supports in siRNA Delivery. / Giammona, Gaetano; Ghersi, Giulio; Mauro, Nicolò; Campora, Simona; Campora, Simona; Ghersi, Giulio; Griffiths, Peter.

In: CHEMICAL ENGINEERING TRANSACTIONS, Vol. 64, 2018, pag. 415-420.

Risultato della ricerca: Article

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AU - Giammona, Gaetano

AU - Ghersi, Giulio

AU - Mauro, Nicolò

AU - Campora, Simona

AU - Campora, Simona

AU - Ghersi, Giulio

AU - Griffiths, Peter

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N2 - The nature of graphene-based nanosystems, as well as the possibility to synthesize them at low cost, have made them in the last few years, an interesting proposition in tumour therapy as a drug delivery system. Here, a reduced form of graphene oxide (RGO) has been synthesized and conjugated with a specific antibody for active targeting against tumour cells (RGOY). Furthermore, its bi-dimensional nature permits also p- p stacking interactions with planar molecules like siRNA (RGOY-siRNA). All these nano-complexes were characterized by DLS and DSC analysis, TEM and Raman spectroscopy and their biocompatibility was demonstrated by viability assay on cell line ECV 304. The system is able to be internalized from cells, as demonstrated by uptake studies with confocal microscopy, performed using its red fluorescence variant (RGOY-AF). A high reduction of the stacking interactions between the graphene sheets was obtained by conjugating RGOY particles to polyvinylpyrrolidone (PVP) (RGOY-PVP). The addition of PVP did not alter the biocompatibility of the system, but limited the formation of aggregates due to the stacking interaction between the graphene sheets: the complexes appeared more dispersed and able to enter into the cells after only few minutes, and in higher amounts with respect to the complex without PVP. All obtained data indicate graphene nanosystems very good candidates as delivery system thanks to their specific properties that permit to link to both antibody and siRNA without any degradation effect.

AB - The nature of graphene-based nanosystems, as well as the possibility to synthesize them at low cost, have made them in the last few years, an interesting proposition in tumour therapy as a drug delivery system. Here, a reduced form of graphene oxide (RGO) has been synthesized and conjugated with a specific antibody for active targeting against tumour cells (RGOY). Furthermore, its bi-dimensional nature permits also p- p stacking interactions with planar molecules like siRNA (RGOY-siRNA). All these nano-complexes were characterized by DLS and DSC analysis, TEM and Raman spectroscopy and their biocompatibility was demonstrated by viability assay on cell line ECV 304. The system is able to be internalized from cells, as demonstrated by uptake studies with confocal microscopy, performed using its red fluorescence variant (RGOY-AF). A high reduction of the stacking interactions between the graphene sheets was obtained by conjugating RGOY particles to polyvinylpyrrolidone (PVP) (RGOY-PVP). The addition of PVP did not alter the biocompatibility of the system, but limited the formation of aggregates due to the stacking interaction between the graphene sheets: the complexes appeared more dispersed and able to enter into the cells after only few minutes, and in higher amounts with respect to the complex without PVP. All obtained data indicate graphene nanosystems very good candidates as delivery system thanks to their specific properties that permit to link to both antibody and siRNA without any degradation effect.

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