Glycation alters ligand binding, enzymatic, and pharmacological properties of human albumin

Valeria Militello, Jennifer Baraka-Vidot, Cynthia Planesse, Emmanuel Bourdon, Philippe Rondeau, Olivier Meilhac, Jean Van Den Elsen

Risultato della ricerca: Article

24 Citazioni (Scopus)

Abstract

Albumin, the major circulating protein in blood plasma, can be subjected to an increased level of glycation in a diabetic context. Albumin exerts crucial pharmacological activities through its drug binding capacity, i.e., ketoprofen, and via its esterase-like activity, allowing the conversion of prodrugs into active drugs. In this study, the impact of the glucose-mediated glycation on the pharmacological and biochemical properties of human albumin was investigated. Aggregation product levels and the redox state were quantified to assess the impact of glycation-mediated changes on the structural properties of albumin. Glucose-mediated changes in ketoprofen binding properties and esterase-like activity were evaluated using fluorescence spectroscopy and p-nitrophenyl acetate hydrolysis assays, respectively. With the exception of oxidative parameters, significant dose-dependent alterations in biochemical and functional properties of in vitro glycated albumin were observed. We also found that the dose-dependent increase in levels of glycation and protein aggregation and average molecular mass changes correlated with a gradual decrease in the affinity of albumin for ketoprofen and its esterase-like property. In parallel, significant alterations in both pharmacological properties were also evidenced in albumin purified from diabetic patients. Partial least-squares regression analyses established a significant correlation between glycation-mediated changes in biochemical and pharmacological properties of albumin, highlighting the important role for glycation in the variability of the drug response in a diabetic situation.
Lingua originaleEnglish
pagine (da-a)3051-3062
Numero di pagine12
RivistaBiochemistry
Volume54
Stato di pubblicazionePublished - 2015

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cita questo

Militello, V., Baraka-Vidot, J., Planesse, C., Bourdon, E., Rondeau, P., Meilhac, O., & Van Den Elsen, J. (2015). Glycation alters ligand binding, enzymatic, and pharmacological properties of human albumin. Biochemistry, 54, 3051-3062.