Genotyping of Legionella pneumophila serogroup 1 strains isolated in Northern Sicily, Italy.

Celestino Bonura, Donatella Ferraro, Cinzia Cala', Alfredo Chiarini, Anna Giammanco, Santina Belfiore, Nicolò Casuccio, Alfredo Chiarini

Risultato della ricerca: Article

10 Citazioni (Scopus)

Abstract

During a three-year period, from April 2002 to May 2005, one hundred-forty-seven samples, taken from technical systems of water distribution at point of use, were repeatedly collected at six different sites in Northern Sicily and assayed for the presence of Legionella pneumophila serogroup 1 and serogroups 2 to 14. At the first samplings, the water distribution systems of all the sites were heavily contaminated, and disinfection treatments by the superheat and flush method were therefore performed. Treatments were always successful against L. pneumophila sg.1, but only in a few cases against all other serogroups. Eighty-six strains of L. pneumophila sg. 1, isolated from 26 of these samples, were characterized by amplified fragment length polymorphism (AFLP) analysis and sequence-based typing (SBT) procedure. Perfectly overlapping results were obtained by both the procedures and four genotypes were identified, accounting for all the isolates. The easy transferability of the SBT data through a web-based database made it possible to identify the presence in Northern Sicily of the two SBT types most commonly circulating in Europe.
Lingua originaleEnglish
pagine (da-a)217-228
Numero di pagine12
RivistaNew Microbiologica
Volume2008-04
Stato di pubblicazionePublished - 2008

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All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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