Genotyping of Legionella pneumophila serogroup 1 strains isolated in Northern Sicily, Italy.

Alfredo Chiarini, Anna Giammanco, Donatella Ferraro, Celestino Bonura, Cinzia Cala', Alfredo Chiarini, Santina Belfiore, Nicolò Casuccio

Risultato della ricerca: Article

10 Citazioni (Scopus)

Abstract

During a three-year period, from April 2002 to May 2005, one hundred-forty-seven samples, taken from technical systems of water distribution at point of use, were repeatedly collected at six different sites in Northern Sicily and assayed for the presence of Legionella pneumophila serogroup 1 and serogroups 2 to 14. At the first samplings, the water distribution systems of all the sites were heavily contaminated, and disinfection treatments by the superheat and flush method were therefore performed. Treatments were always successful against L. pneumophila sg.1, but only in a few cases against all other serogroups. Eighty-six strains of L. pneumophila sg. 1, isolated from 26 of these samples, were characterized by amplified fragment length polymorphism (AFLP) analysis and sequence-based typing (SBT) procedure. Perfectly overlapping results were obtained by both the procedures and four genotypes were identified, accounting for all the isolates. The easy transferability of the SBT data through a web-based database made it possible to identify the presence in Northern Sicily of the two SBT types most commonly circulating in Europe.
Lingua originaleEnglish
pagine (da-a)217-228
Numero di pagine12
RivistaNew Microbiologica
Volume2008-04
Stato di pubblicazionePublished - 2008

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Sicily
Legionella pneumophila
Italy
Amplified Fragment Length Polymorphism Analysis
Water
Disinfection
Genotype
Databases
Serogroup

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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Genotyping of Legionella pneumophila serogroup 1 strains isolated in Northern Sicily, Italy. / Chiarini, Alfredo; Giammanco, Anna; Ferraro, Donatella; Bonura, Celestino; Cala', Cinzia; Chiarini, Alfredo; Belfiore, Santina; Casuccio, Nicolò.

In: New Microbiologica, Vol. 2008-04, 2008, pag. 217-228.

Risultato della ricerca: Article

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abstract = "During a three-year period, from April 2002 to May 2005, one hundred-forty-seven samples, taken from technical systems of water distribution at point of use, were repeatedly collected at six different sites in Northern Sicily and assayed for the presence of Legionella pneumophila serogroup 1 and serogroups 2 to 14. At the first samplings, the water distribution systems of all the sites were heavily contaminated, and disinfection treatments by the superheat and flush method were therefore performed. Treatments were always successful against L. pneumophila sg.1, but only in a few cases against all other serogroups. Eighty-six strains of L. pneumophila sg. 1, isolated from 26 of these samples, were characterized by amplified fragment length polymorphism (AFLP) analysis and sequence-based typing (SBT) procedure. Perfectly overlapping results were obtained by both the procedures and four genotypes were identified, accounting for all the isolates. The easy transferability of the SBT data through a web-based database made it possible to identify the presence in Northern Sicily of the two SBT types most commonly circulating in Europe.",
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AU - Chiarini, Alfredo

AU - Giammanco, Anna

AU - Ferraro, Donatella

AU - Bonura, Celestino

AU - Cala', Cinzia

AU - Chiarini, Alfredo

AU - Belfiore, Santina

AU - Casuccio, Nicolò

PY - 2008

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N2 - During a three-year period, from April 2002 to May 2005, one hundred-forty-seven samples, taken from technical systems of water distribution at point of use, were repeatedly collected at six different sites in Northern Sicily and assayed for the presence of Legionella pneumophila serogroup 1 and serogroups 2 to 14. At the first samplings, the water distribution systems of all the sites were heavily contaminated, and disinfection treatments by the superheat and flush method were therefore performed. Treatments were always successful against L. pneumophila sg.1, but only in a few cases against all other serogroups. Eighty-six strains of L. pneumophila sg. 1, isolated from 26 of these samples, were characterized by amplified fragment length polymorphism (AFLP) analysis and sequence-based typing (SBT) procedure. Perfectly overlapping results were obtained by both the procedures and four genotypes were identified, accounting for all the isolates. The easy transferability of the SBT data through a web-based database made it possible to identify the presence in Northern Sicily of the two SBT types most commonly circulating in Europe.

AB - During a three-year period, from April 2002 to May 2005, one hundred-forty-seven samples, taken from technical systems of water distribution at point of use, were repeatedly collected at six different sites in Northern Sicily and assayed for the presence of Legionella pneumophila serogroup 1 and serogroups 2 to 14. At the first samplings, the water distribution systems of all the sites were heavily contaminated, and disinfection treatments by the superheat and flush method were therefore performed. Treatments were always successful against L. pneumophila sg.1, but only in a few cases against all other serogroups. Eighty-six strains of L. pneumophila sg. 1, isolated from 26 of these samples, were characterized by amplified fragment length polymorphism (AFLP) analysis and sequence-based typing (SBT) procedure. Perfectly overlapping results were obtained by both the procedures and four genotypes were identified, accounting for all the isolates. The easy transferability of the SBT data through a web-based database made it possible to identify the presence in Northern Sicily of the two SBT types most commonly circulating in Europe.

KW - Legionella pneumophila, Surveillance, Water distribution system, Molecular typing, Amplified fragment length polymorphism

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JO - New Microbiologica

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SN - 1121-7138

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