Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis

Giovanni Giammanco, Aziz Japoni, Reza Ranjbar, Sh Farshad, Abdolvahab Alborzi, Mojtaba Anvarinejad

Risultato della ricerca: Article

16 Citazioni (Scopus)

Abstract

BACKGROUND: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E. coli This study investigates the genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. METHODS: During 2008-2009, 90 E. coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis. Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis (PFGE) and their corresponding patterns were compared using dendrogram. RESULTS: Sixty five PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively. Most strains exhi-bited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome sizes of the strains were between 1610-4170 kbp. CONCLUSION: With due attention to these results, genetic patterns showed that the strains had different clonalities and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors.
Lingua originaleEnglish
pagine (da-a)408-416
Numero di pagine9
RivistaIranian Red Crescent Medical Journal
Volume14
Stato di pubblicazionePublished - 2012

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Cystitis
Pyelonephritis
Escherichia coli
Pulsed Field Gel Electrophoresis
Uropathogenic Escherichia coli
Genome Size
Urinary Tract Infections
Genome
Health
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cita questo

Giammanco, G., Japoni, A., Ranjbar, R., Farshad, S., Alborzi, A., & Anvarinejad, M. (2012). Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. Iranian Red Crescent Medical Journal, 14, 408-416.

Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. / Giammanco, Giovanni; Japoni, Aziz; Ranjbar, Reza; Farshad, Sh; Alborzi, Abdolvahab; Anvarinejad, Mojtaba.

In: Iranian Red Crescent Medical Journal, Vol. 14, 2012, pag. 408-416.

Risultato della ricerca: Article

Giammanco, G, Japoni, A, Ranjbar, R, Farshad, S, Alborzi, A & Anvarinejad, M 2012, 'Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis', Iranian Red Crescent Medical Journal, vol. 14, pagg. 408-416.
Giammanco, Giovanni ; Japoni, Aziz ; Ranjbar, Reza ; Farshad, Sh ; Alborzi, Abdolvahab ; Anvarinejad, Mojtaba. / Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. In: Iranian Red Crescent Medical Journal. 2012 ; Vol. 14. pagg. 408-416.
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AU - Giammanco, Giovanni

AU - Japoni, Aziz

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AU - Alborzi, Abdolvahab

AU - Anvarinejad, Mojtaba

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N2 - BACKGROUND: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E. coli This study investigates the genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. METHODS: During 2008-2009, 90 E. coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis. Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis (PFGE) and their corresponding patterns were compared using dendrogram. RESULTS: Sixty five PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively. Most strains exhi-bited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome sizes of the strains were between 1610-4170 kbp. CONCLUSION: With due attention to these results, genetic patterns showed that the strains had different clonalities and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors.

AB - BACKGROUND: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E. coli This study investigates the genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. METHODS: During 2008-2009, 90 E. coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis. Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis (PFGE) and their corresponding patterns were compared using dendrogram. RESULTS: Sixty five PFGE profiles were obtained from the genome of E. coli strains by this genotyping method. Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively. Most strains exhi-bited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate. Genome sizes of the strains were between 1610-4170 kbp. CONCLUSION: With due attention to these results, genetic patterns showed that the strains had different clonalities and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors.

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